We are analyzing https://link.springer.com/article/10.1007/s10811-007-9263-7.

Title:
Purification and characterization of phycoferritin from the blue-green alga, Arthrospira (Spirulina) platensis | Journal of Applied Phycology
Description:
Phycoferritin from the nutritionally important blue-green alga Arthrospira platensis has been isolated, by application of conventional biochemical techniques. The molecular mass, yield, iron and total neutral carbohydrate contents of the purified protein were 470 kDa, 0.044 mg g−1 of Arthrospira, 1.4 and 20%, respectively. The iron content was much lower when compared to bacterial and mammalian ferritins. The P: Fe ratio of Arthrospira phycoferritin was 1: 3.5, a value akin to bacterioferritins. Native gel-electrophoresis revealed the presence of isoforms. Subunit analysis by SDS-PAGE and Western blotting showed a protein subunit with an apparent molecular mass of 18 kDa. Oligomeric forms of the protein subunit were also present. The phycoferritin exhibited cross-reactivity with anti-pea seed ferritin suggesting phylogenetic relationship with that of higher plants. Carbohydrate analysis of phycoferritin by GC-MS revealed the presence of sugars such as galactose, glucose and mannose similar to that of mammalian ferritins. Interestingly, the analysis also revealed sugars such as rhamnose, xylose and talose, which has not been reported in the structure of ferritins. Except for very low histidine content in phycoferritin, the rest of the amino acid composition resembled to ferritins of other species. UV-visible spectral analysis of the phycoferritin revealed the presence of haem groups, a property characteristic of bacterioferritins. The fluorescence intensity of phycoferritin was higher than equine spleen ferritin. Circular dichroic spectra revealed a lower degree of helicity.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Books & Literature

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

scholar, google, article, cas, pubmed, iron, ferritin, biochem, purification, analysis, characterization, phycoferritin, ferritins, chem, bacterioferritin, content, arthrospira, deshpande, protein, subunit, biol, research, proteins, privacy, cookies, journal, sashidhar, revealed, access, hyderabad, function, publish, search, platensis, structure, properties, plant, lett, storage, biophys, anal, nature, data, policy, information, log, bluegreen, alga, lakshmi, deepa,

Topics {✒️}

month download article/chapter phycoferritin exhibited cross-reactivity uv-visible spectral analysis native gel-electrophoresis revealed blue-green alga full article pdf conventional biochemical techniques bhanu prakash reddy privacy choices/manage cookies pea seed ferritin theil ec applied phycology aims related subjects research scholar conditions privacy policy iron detoxification properties low histidine content cd spectral analysis low iron environments amino acid analysis heme iron proteins native iron-cores european economic area scope submit manuscript western blotting showed genome-wide comparison le brun ne horse spleen apoferritin indian opium based folin phenol reagent azobacter cytochrome b557 iron-binding protein sashidhar & vijay deshpande equine spleen ferritin arthrospira platensis m2 neisseria gonorrhoeae bacterioferritin human serum proteins iron-fortified arthrospira article journal accepting optional cookies plants ectopically expressing gc-ms revealed apparent molecular mass journal finder publish article log heme iron nutrition check access instant access gc-ms analysis escherichia coli bacterioferritin

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Purification and characterization of phycoferritin from the blue-green alga, Arthrospira (Spirulina) platensis
         description:Phycoferritin from the nutritionally important blue-green alga Arthrospira platensis has been isolated, by application of conventional biochemical techniques. The molecular mass, yield, iron and total neutral carbohydrate contents of the purified protein were 470 kDa, 0.044 mg g−1 of Arthrospira, 1.4 and 20%, respectively. The iron content was much lower when compared to bacterial and mammalian ferritins. The P: Fe ratio of Arthrospira phycoferritin was 1: 3.5, a value akin to bacterioferritins. Native gel-electrophoresis revealed the presence of isoforms. Subunit analysis by SDS-PAGE and Western blotting showed a protein subunit with an apparent molecular mass of 18 kDa. Oligomeric forms of the protein subunit were also present. The phycoferritin exhibited cross-reactivity with anti-pea seed ferritin suggesting phylogenetic relationship with that of higher plants. Carbohydrate analysis of phycoferritin by GC-MS revealed the presence of sugars such as galactose, glucose and mannose similar to that of mammalian ferritins. Interestingly, the analysis also revealed sugars such as rhamnose, xylose and talose, which has not been reported in the structure of ferritins. Except for very low histidine content in phycoferritin, the rest of the amino acid composition resembled to ferritins of other species. UV-visible spectral analysis of the phycoferritin revealed the presence of haem groups, a property characteristic of bacterioferritins. The fluorescence intensity of phycoferritin was higher than equine spleen ferritin. Circular dichroic spectra revealed a lower degree of helicity.
         datePublished:2007-10-30T00:00:00Z
         dateModified:2007-10-30T00:00:00Z
         pageStart:359
         pageEnd:366
         sameAs:https://doi.org/10.1007/s10811-007-9263-7
         keywords:
            Bacterioferritin
            Cross-reactivity
            Ferritin
            Glycoprotein
            Haem
            Subunit
            Plant Sciences
            Freshwater & Marine Ecology
            Plant Physiology
            Ecology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig5_HTML.gif
         isPartOf:
            name:Journal of Applied Phycology
            issn:
               1573-5176
               0921-8971
            volumeNumber:20
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Netherlands
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:G. Lakshmi Deepa
               affiliation:
                     name:University College of Science, Osmania University
                     address:
                        name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:R. B. Sashidhar
               affiliation:
                     name:University College of Science, Osmania University
                     address:
                        name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Vijay Deshpande
               affiliation:
                     name:University College of Science, Osmania University
                     address:
                        name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Purification and characterization of phycoferritin from the blue-green alga, Arthrospira (Spirulina) platensis
      description:Phycoferritin from the nutritionally important blue-green alga Arthrospira platensis has been isolated, by application of conventional biochemical techniques. The molecular mass, yield, iron and total neutral carbohydrate contents of the purified protein were 470 kDa, 0.044 mg g−1 of Arthrospira, 1.4 and 20%, respectively. The iron content was much lower when compared to bacterial and mammalian ferritins. The P: Fe ratio of Arthrospira phycoferritin was 1: 3.5, a value akin to bacterioferritins. Native gel-electrophoresis revealed the presence of isoforms. Subunit analysis by SDS-PAGE and Western blotting showed a protein subunit with an apparent molecular mass of 18 kDa. Oligomeric forms of the protein subunit were also present. The phycoferritin exhibited cross-reactivity with anti-pea seed ferritin suggesting phylogenetic relationship with that of higher plants. Carbohydrate analysis of phycoferritin by GC-MS revealed the presence of sugars such as galactose, glucose and mannose similar to that of mammalian ferritins. Interestingly, the analysis also revealed sugars such as rhamnose, xylose and talose, which has not been reported in the structure of ferritins. Except for very low histidine content in phycoferritin, the rest of the amino acid composition resembled to ferritins of other species. UV-visible spectral analysis of the phycoferritin revealed the presence of haem groups, a property characteristic of bacterioferritins. The fluorescence intensity of phycoferritin was higher than equine spleen ferritin. Circular dichroic spectra revealed a lower degree of helicity.
      datePublished:2007-10-30T00:00:00Z
      dateModified:2007-10-30T00:00:00Z
      pageStart:359
      pageEnd:366
      sameAs:https://doi.org/10.1007/s10811-007-9263-7
      keywords:
         Bacterioferritin
         Cross-reactivity
         Ferritin
         Glycoprotein
         Haem
         Subunit
         Plant Sciences
         Freshwater & Marine Ecology
         Plant Physiology
         Ecology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10811-007-9263-7/MediaObjects/10811_2007_9263_Fig5_HTML.gif
      isPartOf:
         name:Journal of Applied Phycology
         issn:
            1573-5176
            0921-8971
         volumeNumber:20
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:G. Lakshmi Deepa
            affiliation:
                  name:University College of Science, Osmania University
                  address:
                     name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:R. B. Sashidhar
            affiliation:
                  name:University College of Science, Osmania University
                  address:
                     name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Vijay Deshpande
            affiliation:
                  name:University College of Science, Osmania University
                  address:
                     name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Journal of Applied Phycology
      issn:
         1573-5176
         0921-8971
      volumeNumber:20
Organization:
      name:Springer Netherlands
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University College of Science, Osmania University
      address:
         name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
         type:PostalAddress
      name:University College of Science, Osmania University
      address:
         name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
         type:PostalAddress
      name:University College of Science, Osmania University
      address:
         name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:G. Lakshmi Deepa
      affiliation:
            name:University College of Science, Osmania University
            address:
               name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
               type:PostalAddress
            type:Organization
      name:R. B. Sashidhar
      affiliation:
            name:University College of Science, Osmania University
            address:
               name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
               type:PostalAddress
            type:Organization
      name:Vijay Deshpande
      affiliation:
            name:University College of Science, Osmania University
            address:
               name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
      name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
      name:Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(122)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

4.1s.

LINK . SPRINGER . COM {}