We are analyzing https://link.springer.com/article/10.1007/s10695-015-0037-x.

Title:
Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale | Fish Physiology and Biochemistry
Description:
A new cell line was established from half-smooth tongue sole Cynoglossus semilaevis pseudomale gonad (CSPMG). Primary culture was initiated from gonad tissues pieces, and the CSPMG cells were cultured at 24 °C in Dulbecco’s modified Eagle medium/F12 medium (1:1) (pH7.0), supplemented with 20 % fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, insulin-like growth factor-I, 2-mercaptoethanol, penicillin and streptomycin. The cultured CSPMG cells, in fibroblast shape, proliferated to 100 % confluency 10 days later and had been subcultured to passage 109. Chromosome analyses indicated that the CSPMG cells exhibited chromosomal aneuploidy with a modal chromosome number of 42, which displayed the normal diploid karyotype of half-smooth tongue sole (2n = 42t, NF = 42). Reverse transcription polymerase chain reaction revealed CSPMG cells could express gonad somatic cell functional genes Sox9a, Wt1a and weakly germ cell marker gene Vasa, but not male specific gene Dmrt1. Transfection experiment demonstrated that CSPMG cells transfected with pEGFP-N3 plasmid and small RNA could express green and red fluorescence signals with high transfection efficiency. In conclusion, a continuous CSPMG cell line has been established successfully. The cell line might serve as a valuable tool for studies on the mechanism of sex determination, sex reversal and gonad development in flatfish.
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Keywords {🔍}

article, scholar, google, pubmed, cas, cell, wang, sex, fish, line, chen, characterization, cynoglossus, semilaevis, establishment, tongue, sole, halfsmooth, liu, expression, cells, gene, zhang, sha, reversal, sun, development, yang, biochem, gonad, cspmg, growth, genes, differentiation, germ, molecular, aquaculture, privacy, cookies, content, analysis, gao, culture, soxa, transfection, determination, oreochromis, kobayashi, nagahama, nile,

Topics {✒️}

na wang & zhen-xia sha half-smooth tongue sole half-smooth tongue-sole month download article/chapter female-specific aflp markers zebrafish germ cells zebrafish anti-müllerian hormone foreign gene transfection androgen-treated nile tilapia article fish physiology somatic cells specification androgen-induced sex reversal hormone-induced sex reversal transfection experiment demonstrated high transfection efficiency germ cell differentiation cultured cspmg cells chinese tongue sole cspmg cells transfected head kidney full article pdf embryonic cells neural stem cells sanchez de melo gonad cell line duplicated zebrafish chromosomes privacy choices/manage cookies similar transcription activators fish cell line cell culture system continuous cell line fin cell line small rna brown-marbled grouper state 863 high-technology cell line derived splenic cell line spleen cell line testicular cell line gonad tissues pieces cspmg cells cynoglossus semilaevis express green heart-cell line normal diploid karyotype comp biochem phys similar gene structure european economic area modal chromosome number pegfp-n3 plasmid

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