We are analyzing https://link.springer.com/article/10.1007/s10616-007-9100-5.

Title:
Direct interaction of Cucurbitacin E isolated from Alsomitra macrocarpa to actin filament | Cytotechnology
Description:
A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.
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Keywords {🔍}

article, actin, google, scholar, cas, cell, cucurbitacin, cytoskeleton, kyoto, macrocarpa, cuc, access, privacy, cookies, content, alsomitra, cells, chem, publish, research, search, filament, kioka, biol, school, author, japan, data, information, log, journal, cytotechnology, isolated, momma, masuzawa, nakai, noriyuki, masaya, nagao, human, open, related, discover, factin, products, structures, elaterium, duncan, jasplakinolide, polymerization,

Topics {✒️}

month download article/chapter actin-targeting natural products effective anti-cancer agent cucurbitacin e-induced disruption article cytotechnology aims noriyuki kioka full article pdf privacy choices/manage cookies cell morphology induces actin polymerization actin targeting peptide stabilizes actin filament direct interaction author information authors pyrene labelled actin cytotoxic natural product anti-inflammatory principle applied life sciences prostate carcinoma cells hsc-t6 cells masaya nagao inhibits cell growth european economic area standard fluorescence assay related subjects green alga micrasterias f-actin aggregates potent cytotoxic macrolide 13c nmr spectroscopy o'byrne sm check access instant access conditions privacy policy alsomitra macrocarpa leaves actin stabilizing drugs similar morphologic alterations biological activities mount sinai school article momma accepting optional cookies actin cytoskeleton vinculin-binding protein article log duncan kl journal finder publish cell lines globular actin actin filament moeko chujo yasunori kiyama

Schema {🗺️}

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         description:A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.
         datePublished:2007-11-07T00:00:00Z
         dateModified:2007-11-07T00:00:00Z
         pageStart:33
         pageEnd:39
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            Cucurbitacin E
            Cell morphology
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            Cytoskeleton
            Biotechnology
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            general
            Biochemistry
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      headline:Direct interaction of Cucurbitacin E isolated from Alsomitra macrocarpa to actin filament
      description:A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.
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      pageEnd:39
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         Cucurbitacin E
         Cell morphology
         Actin
         Cytoskeleton
         Biotechnology
         Biomedicine
         general
         Biochemistry
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