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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
  12. Libraries
  13. CDN Services

We are analyzing https://link.springer.com/article/10.1007/s10585-007-9060-3.

Title:
Chemokine expression is associated with the accumulation of tumour associated macrophages (TAMs) and progression in human colorectal cancer | Clinical & Experimental Metastasis
Description:
Chemokines promote tumour progression by enhancing proliferation and modifying the immune response. The purpose of this study was to test the hypothesis that CCL2 monocyte chemotactic protein-1 (MCP-1) contributes to the progression of colorectal cancer by influencing the number and distribution of tumour associated macrophages (TAMs). Chemokine expression was assessed in human colorectal adenocarcinomas by ribonuclease protection assay (RPA). Colonic adenocarcinoma cell lines were used to assess chemokine production by enzyme linked immunosorbant assay (ELISA), and Boyden microchemotaxis assays were performed to determine cell line supernatant monocyte chemotactic activity. CCL2 production was assessed in paraffin embedded tumour samples by immunohistochemistry. Finally, the number of macrophages and their distribution was determined in the same colorectal adenocarcinomas and compared with CCL2 expression and tumour stage. Results showed that CCL2 produced by cell lines induced monocyte chemoattraction, the expression of this chemokine in solid cancers increased with tumour stage (P < 0.05) and immunohistochemistry localized production to tumour cells. Analysis of the macrophage infiltrate showed that the accumulation was significantly greater in tumours than controls (P < 0.005) and within tumours it was greatest in necrotic regions (median 44,600 per mm3). Macrophage accumulation increased with tumour stage and correlated with CCL2 expression (r s = 0.8). CXCL8 interleukin 8 (IL-8), a potent angiogenic factor and growth factor, was expressed in all tumours and cell lines. It is concluded that CCL2 induces the accumulation of tumour promoting TAMs in human colorectal cancer and represents a therapeutic target to modify the macrophage response and direct immune mediated therapy.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
  • Health & Fitness
  • Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

google, scholar, cancer, pubmed, cas, article, tumour, macrophages, chemokine, human, colorectal, macrophage, expression, cell, progression, protein, chemokines, factor, negus, ccl, mcp, res, monocyte, growth, access, privacy, cookies, content, research, accumulation, bailey, chemotactic, interleukin, therapy, cytokine, immunol, role, mantovani, breast, angiogenesis, london, publish, search, metastasis, tams, charles, immune, assay, tumours, colon,

Topics {✒️}

month download article/chapter chemokine/chemokine receptor nomenclature single-cell immune landscape tumor-derived chemotactic factor related subjects human colorectal cancer full article pdf monocyte chemoattractant protein-1 colorectal cancer diagnostics autocrine growth factor privacy choices/manage cookies potent angiogenic factor human colorectal adenocarcinomas inflammatory cell populations human bladder cancer human breast cancer ribonuclease protection assay human ovarian cancer neutrophil-activating protein macrophage chemoattractant protein-1 tumour promoting tams anti-cancer therapy spontaneous cancer metastases human ovarian carcinoma negus rpm assess chemokine production article bailey boyden microchemotaxis assays solid cancers increased random sampling methods random plane sections muramyl dipeptide encapsulated advanced breast carcinoma conditions privacy policy macrophages promote angiogenesis colorectal cancer uridine-5′-triphosphate vegf lymphocyte-derived cytokines normal colon deliver gene therapy growth factor article log macrophage peritumoral infiltration accepting optional cookies european economic area robert goldin lewis ce cc chemokine regulated check access instant access

Questions {❓}

  • Balkwill F, Mantovani A (2001) Inflammation and cancer: back to Virchow?
  • Scotton CJ, Wilson JL, Milliken D et al (2001) Epithelial cancer cell migration: a role for chemokine receptors?

Schema {🗺️}

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         headline:Chemokine expression is associated with the accumulation of tumour associated macrophages (TAMs) and progression in human colorectal cancer
         description:Chemokines promote tumour progression by enhancing proliferation and modifying the immune response. The purpose of this study was to test the hypothesis that CCL2 monocyte chemotactic protein-1 (MCP-1) contributes to the progression of colorectal cancer by influencing the number and distribution of tumour associated macrophages (TAMs). Chemokine expression was assessed in human colorectal adenocarcinomas by ribonuclease protection assay (RPA). Colonic adenocarcinoma cell lines were used to assess chemokine production by enzyme linked immunosorbant assay (ELISA), and Boyden microchemotaxis assays were performed to determine cell line supernatant monocyte chemotactic activity. CCL2 production was assessed in paraffin embedded tumour samples by immunohistochemistry. Finally, the number of macrophages and their distribution was determined in the same colorectal adenocarcinomas and compared with CCL2 expression and tumour stage. Results showed that CCL2 produced by cell lines induced monocyte chemoattraction, the expression of this chemokine in solid cancers increased with tumour stage (P < 0.05) and immunohistochemistry localized production to tumour cells. Analysis of the macrophage infiltrate showed that the accumulation was significantly greater in tumours than controls (P < 0.005) and within tumours it was greatest in necrotic regions (median 44,600 per mm3). Macrophage accumulation increased with tumour stage and correlated with CCL2 expression (r s = 0.8). CXCL8 interleukin 8 (IL-8), a potent angiogenic factor and growth factor, was expressed in all tumours and cell lines. It is concluded that CCL2 induces the accumulation of tumour promoting TAMs in human colorectal cancer and represents a therapeutic target to modify the macrophage response and direct immune mediated therapy.
         datePublished:2007-03-28T00:00:00Z
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      headline:Chemokine expression is associated with the accumulation of tumour associated macrophages (TAMs) and progression in human colorectal cancer
      description:Chemokines promote tumour progression by enhancing proliferation and modifying the immune response. The purpose of this study was to test the hypothesis that CCL2 monocyte chemotactic protein-1 (MCP-1) contributes to the progression of colorectal cancer by influencing the number and distribution of tumour associated macrophages (TAMs). Chemokine expression was assessed in human colorectal adenocarcinomas by ribonuclease protection assay (RPA). Colonic adenocarcinoma cell lines were used to assess chemokine production by enzyme linked immunosorbant assay (ELISA), and Boyden microchemotaxis assays were performed to determine cell line supernatant monocyte chemotactic activity. CCL2 production was assessed in paraffin embedded tumour samples by immunohistochemistry. Finally, the number of macrophages and their distribution was determined in the same colorectal adenocarcinomas and compared with CCL2 expression and tumour stage. Results showed that CCL2 produced by cell lines induced monocyte chemoattraction, the expression of this chemokine in solid cancers increased with tumour stage (P < 0.05) and immunohistochemistry localized production to tumour cells. Analysis of the macrophage infiltrate showed that the accumulation was significantly greater in tumours than controls (P < 0.005) and within tumours it was greatest in necrotic regions (median 44,600 per mm3). Macrophage accumulation increased with tumour stage and correlated with CCL2 expression (r s = 0.8). CXCL8 interleukin 8 (IL-8), a potent angiogenic factor and growth factor, was expressed in all tumours and cell lines. It is concluded that CCL2 induces the accumulation of tumour promoting TAMs in human colorectal cancer and represents a therapeutic target to modify the macrophage response and direct immune mediated therapy.
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               type:PostalAddress
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      name:Ara Darzi
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            name:St Mary’s Hospital, Imperial College of Science, Technology, and Medicine
            address:
               name:Department of Surgical Oncology and Technology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
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      name:Department of Surgical Oncology and Technology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
      name:Department of Colorectal Surgery, Level 2, John Radcliffe Hospital, Oxford, UK
      name:Department of Gastroenterology, St Mary’s Hospital, London, UK
      name:Department of Surgical Oncology and Technology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
      name:Department of Surgical Oncology and Technology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
      name:Department of Pathology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
      name:Istituto Clinico Humanitas, Rozzano, Milan, Italy
      name:Department of Surgical Oncology and Technology, St Mary’s Hospital, Imperial College of Science, Technology, and Medicine, London, UK
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External Links {🔗}(135)

Analytics and Tracking {📊}

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Libraries {📚}

  • Clipboard.js
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CDN Services {📦}

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4.78s.