Here's how LINK.SPRINGER.COM makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Schema
  9. External Links
  10. Analytics And Tracking
  11. Libraries
  12. CDN Services

We are analyzing https://link.springer.com/article/10.1007/s10549-011-1373-x.

Title:
Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients | Breast Cancer Research and Treatment
Description:
Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Health & Fitness
  • Science
  • Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,603,474 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

cancer, article, cells, breast, google, scholar, pubmed, cas, circulating, tumor, transition, patients, ctcs, expression, epithelialmesenchymal, stem, cell, emt, disease, detection, access, res, rome, research, mesenchymal, privacy, cookies, content, stemness, gazzaniga, epithelial, publish, search, study, raimondi, gradilone, naso, cortesi, epcam, markers, found, vimentin, fibronectin, discover, metastatic, open, molecular, cristofanilli, clinical, pantel,

Topics {✒️}

cancer stem cells month download article/chapter stem cell signaling epithelial–mesenchymal transition markers epithelial-mesenchymal transition epithelial mesenchymal transition node-positive breast cancer disseminated tumor cells circulating tumor cells metastatic breast cancer circulating cancer cells high ep-cam expression negative ck/cd45 expression disseminating tumour cells full article pdf related subjects mesenchymal transition privacy choices/manage cookies circulating tumour cell breast cancer patients human breast cancer eribulin-based treatment article raimondi paola gazzaniga check access instant access cancer research 69 european economic area undergone intrinsic modifications cellection™ dynabeads® coated hayes df specific biological properties international expert consensus chemosensitivity profile assay conditions privacy policy mesenchymal transitions meeting abstract supplement campus biomedico university electronic supplementary material exclude cells detected cells samples negative accepting optional cookies samples found positive found statistically significant bone marrow micrometastasis article log breast cancer eradicate micrometastatic disease journal finder publish

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients
         description:Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
         datePublished:2011-02-05T00:00:00Z
         dateModified:2011-02-05T00:00:00Z
         pageStart:449
         pageEnd:455
         sameAs:https://doi.org/10.1007/s10549-011-1373-x
         keywords:
            Circulating tumor cells
            Epithelial-mesenchymal transition
            Cancer stem cells
            Cytokeratins
            Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-011-1373-x/MediaObjects/10549_2011_1373_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-011-1373-x/MediaObjects/10549_2011_1373_Fig2_HTML.gif
         isPartOf:
            name:Breast Cancer Research and Treatment
            issn:
               1573-7217
               0167-6806
            volumeNumber:130
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Cristina Raimondi
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Angela Gradilone
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Giuseppe Naso
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bruno Vincenzi
               affiliation:
                     name:Campus Biomedico University of Rome
                     address:
                        name:Division of Medical Oncology, Campus Biomedico University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Arianna Petracca
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chiara Nicolazzo
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Antonella Palazzo
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Rosa Saltarelli
               affiliation:
                     name:Hospital “S. Giovanni Evangelista”
                     address:
                        name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Franco Spremberg
               affiliation:
                     name:Hospital “S. Giovanni Evangelista”
                     address:
                        name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Enrico Cortesi
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Paola Gazzaniga
               affiliation:
                     name:Sapienza University of Rome
                     address:
                        name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients
      description:Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
      datePublished:2011-02-05T00:00:00Z
      dateModified:2011-02-05T00:00:00Z
      pageStart:449
      pageEnd:455
      sameAs:https://doi.org/10.1007/s10549-011-1373-x
      keywords:
         Circulating tumor cells
         Epithelial-mesenchymal transition
         Cancer stem cells
         Cytokeratins
         Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-011-1373-x/MediaObjects/10549_2011_1373_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-011-1373-x/MediaObjects/10549_2011_1373_Fig2_HTML.gif
      isPartOf:
         name:Breast Cancer Research and Treatment
         issn:
            1573-7217
            0167-6806
         volumeNumber:130
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Cristina Raimondi
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Angela Gradilone
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Giuseppe Naso
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bruno Vincenzi
            affiliation:
                  name:Campus Biomedico University of Rome
                  address:
                     name:Division of Medical Oncology, Campus Biomedico University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Arianna Petracca
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chiara Nicolazzo
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Antonella Palazzo
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Rosa Saltarelli
            affiliation:
                  name:Hospital “S. Giovanni Evangelista”
                  address:
                     name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Franco Spremberg
            affiliation:
                  name:Hospital “S. Giovanni Evangelista”
                  address:
                     name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Enrico Cortesi
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Paola Gazzaniga
            affiliation:
                  name:Sapienza University of Rome
                  address:
                     name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Breast Cancer Research and Treatment
      issn:
         1573-7217
         0167-6806
      volumeNumber:130
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Sapienza University of Rome
      address:
         name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Campus Biomedico University of Rome
      address:
         name:Division of Medical Oncology, Campus Biomedico University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Hospital “S. Giovanni Evangelista”
      address:
         name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
         type:PostalAddress
      name:Hospital “S. Giovanni Evangelista”
      address:
         name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
      name:Sapienza University of Rome
      address:
         name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Cristina Raimondi
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Angela Gradilone
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Giuseppe Naso
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Bruno Vincenzi
      affiliation:
            name:Campus Biomedico University of Rome
            address:
               name:Division of Medical Oncology, Campus Biomedico University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Arianna Petracca
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Chiara Nicolazzo
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Antonella Palazzo
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Rosa Saltarelli
      affiliation:
            name:Hospital “S. Giovanni Evangelista”
            address:
               name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Franco Spremberg
      affiliation:
            name:Hospital “S. Giovanni Evangelista”
            address:
               name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Enrico Cortesi
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Paola Gazzaniga
      affiliation:
            name:Sapienza University of Rome
            address:
               name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
      name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
      name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
      name:Division of Medical Oncology, Campus Biomedico University of Rome, Rome, Italy
      name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
      name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
      name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
      name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
      name:Division of Medical Oncology, Hospital “S. Giovanni Evangelista”, Tivoli, Rome, Italy
      name:Division of Medical Oncology, Sapienza University of Rome, Rome, Italy
      name:Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(101)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Clipboard.js
  • Prism.js

CDN Services {📦}

  • Crossref

3.6s.