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Title:
Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients | Breast Cancer Research and Treatment
Description:
Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
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cancer, article, cells, breast, google, scholar, pubmed, cas, circulating, tumor, transition, patients, ctcs, expression, epithelialmesenchymal, stem, cell, emt, disease, detection, access, res, rome, research, mesenchymal, privacy, cookies, content, stemness, gazzaniga, epithelial, publish, search, study, raimondi, gradilone, naso, cortesi, epcam, markers, found, vimentin, fibronectin, discover, metastatic, open, molecular, cristofanilli, clinical, pantel,
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cancer stem cells month download article/chapter stem cell signaling epithelial–mesenchymal transition markers epithelial-mesenchymal transition epithelial mesenchymal transition node-positive breast cancer disseminated tumor cells circulating tumor cells metastatic breast cancer circulating cancer cells high ep-cam expression negative ck/cd45 expression disseminating tumour cells full article pdf related subjects mesenchymal transition privacy choices/manage cookies circulating tumour cell breast cancer patients human breast cancer eribulin-based treatment article raimondi paola gazzaniga check access instant access cancer research 69 european economic area undergone intrinsic modifications cellection™ dynabeads® coated hayes df specific biological properties international expert consensus chemosensitivity profile assay conditions privacy policy mesenchymal transitions meeting abstract supplement campus biomedico university electronic supplementary material exclude cells detected cells samples negative accepting optional cookies samples found positive found statistically significant bone marrow micrometastasis article log breast cancer eradicate micrometastatic disease journal finder publish
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headline:Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients
description:Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
datePublished:2011-02-05T00:00:00Z
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Circulating tumor cells
Epithelial-mesenchymal transition
Cancer stem cells
Cytokeratins
Oncology
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headline:Epithelial-mesenchymal transition and stemness features in circulating tumor cells from breast cancer patients
description:Currently used methods to detect and enumerate circulating tumor cells (CTCs) rely on the expression of the epithelial cell adhesion molecule (EpCAM) and cytokeratins. This selection may exclude cells that have undergone intrinsic modifications of their phenotype, as epithelial-mesenchymal transition (EMT). Aim of the study was to investigate the expression of EMT and stemness markers in CTCs from breast cancer patients in all stages of disease. 92 female breast cancer patients were enrolled. CTCs were isolated by CELLection™ Dynabeads® coated with the monoclonal antibody toward EpCam. Samples found positive for CTCs presence (CD45−/CK+) were evaluated for the expression of ER alpha, HER2, ALDH1, vimentin, and fibronectin. Samples negative for CTCs presence (CD45−/CK−) were also evaluated for the expression of vimentin and fibronectin, used as markers of EMT. CTCs were found in 66% of patients. The distribution of CTCs presence according to stage and grade of disease was found statistically significant. The expression of ALDH1 on CTCs was found to correlate to stage of disease and to the expression of vimentin and fibronectin. In 34% of patients, we detected cells with negative CK/CD45 expression but positive expression of vimentin and fibronectin. There is an urgent need for optimizing CTCs detection methods through the inclusion of EMT markers. The detection of cells in mesenchymal transition, retaining EMT and stemness features, may contribute to discover additional therapeutic targets useful to eradicate micrometastatic disease in breast cancer.
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Epithelial-mesenchymal transition
Cancer stem cells
Cytokeratins
Oncology
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