We are analyzing https://link.springer.com/article/10.1007/s10549-006-9392-8.

Title:
MDA-MB-435 cells are derived from M14 Melanoma cells––a loss for breast cancer, but a boon for melanoma research | Breast Cancer Research and Treatment
Description:
Background The tissue of origin of the cell line MDA-MB-435 has been a matter of debate since analysis of DNA microarray data led Ross et al. (2000, Nat Genet 24(3):227–235) to suggest they might be of melanocyte origin due to their similarity to melanoma cell lines. We have previously shown that MDA-MB-435 cells maintained in multiple laboratories are of common origin to those used by Ross et al. and concluded that MDA-MB-435 cells are not a representative model for breast cancer. We could not determine, however, whether the melanoma-like properties of the MDA-MB-435 cell line are the result of misclassification or due to transdifferention to a melanoma-like phenotype. Methods We used karyotype, comparative genomic hybridization (CGH), and microsatalite polymorphism analyses, combined with bioinformatics analysis of gene expression and single nucleotide polymorphism (SNP) data, to test the hypothesis that the MDA-MB-435 cell line is derived from the melanoma cell line M14. Results We show that the MDA-MB-435 and M14 cell lines are essentially identical with respect to cytogenetic characteristics as well as gene expression patterns and that the minor differences found can be explained by phenotypic and genotypic clonal drift. Conclusions All currently available stocks of MDA-MB-435 cells are derived from the M14 melanoma cell line and can no longer be considered a model of breast cancer. These cells are still a valuable system for the study of cancer metastasis and the extensive literature using these cells since 1982 represent a valuable new resource for the melanoma research community.
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article, cancer, cell, google, scholar, pubmed, breast, cas, cells, mdamb, melanoma, lines, research, line, expression, human, analysis, access, data, medical, center, privacy, cookies, content, derived, rae, origin, gene, metastasis, res, usa, publish, search, creighton, genomic, open, stem, clin, university, information, log, journal, james, meck, ross, genet, properties, comparative, polymorphism, bioinformatics,

Topics {✒️}

month download article/chapter mda-mb-435 cells maintained cell line mda-mb-435 mda-mb-435 cell line java treeview––extensible visualization cancer cell lines m14 melanoma cells m14 cell lines mda-mb-435 cells genome-wide expression patterns melanoma cell lines lcc15-mb cells prostate cell lines full article pdf melanoma research community privacy choices/manage cookies comparative genomic hybridization chemically defined medium microsatalite polymorphism analyses single nucleotide polymorphism michigan medical center human cytogenetic nomenclature general medical sciences breast cancer european economic area cgh minor differences found genotypic clonal drift enzyme activity profiles gene expression patterns conditions privacy policy check access instant access melanoma research article rae eisen mb accepting optional cookies article log organ-specific metastasis author information authors gene expression profiling melanoma properties malignant melanoma hybrid clones derived cancer res 64 cancer res 50 cancer res 36 mda-mb-435 journal finder publish article cite

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