We are analyzing https://link.springer.com/article/10.1007/s10238-014-0319-0.

Title:
PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro | Clinical and Experimental Medicine
Description:
Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) from pancreas-derived mesenchymal stem cells (pMSCs). pMSCs were isolated from discarded pancreatic tissue in the filter liquor during islet isolation procedure in mice and ex vivo expanded in culture. IPCs were induced by transfection of pancreas and duodenal transcription factor 1 (PDX-1) mRNA in vitro. Some islet characteristics were identified on pMSC-derived IPCs in mRNA and protein levels. Our results demonstrated that mouse pMSCs can be transdifferentiated into effective glucose-responsive insulin-producing cells through transfecting synthetic modified PDX-1 mRNA in vitro. The study of PDX-1 mRNA-induced pMSC reprogramming may pave the way toward the development of a novel β-cell source for the treatment of diabetes.
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Keywords {🔍}

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Topics {✒️}

liu jiao bian embryonic stem cells pluripotent stem cells month download article/chapter pdx-1 mrna-induced reprogramming mesenchymal stem cells cells—islet-derived mscs generate insulin-producing cells glucose-stimulated insulin secretion dong sheng li cell stem cell insulin-expressing cells pancreas islet-releasing factors insulin-producing cell differentiation mesenchymal stromal cells hormone-expressing cells insulin-producing cells epithelial-mesenchymal transition insulin-positive cells preferential lineage-specific differentiation human liver-derived dan dan zou beta cells regeneration stem cells islet-derived mscs lebastchi ah isolated cells contained pancreatic cancer cells human pancreas due full article pdf beta-cell death synthetic modified mrna insulin-producing clusters pdx1 mediates differentiation human islet isolation privacy choices/manage cookies highly efficient reprogramming human pancreatic islets pmsc-derived ipcs experimental medicine aims bone marrow mrna 5′ cap-analogues xiao li wang mesenchymal transition developing mouse telencephalon islet-derived fibroblast article guo pancreatic islet transplantation discarded pancreatic tissue human cells

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Extra views are better islet cell precursors generated by epithelial-to-mesenchymal transition?
Transcription factors regulating ?

Schema {🗺️}

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         description: Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) from pancreas-derived mesenchymal stem cells (pMSCs). pMSCs were isolated from discarded pancreatic tissue in the filter liquor during islet isolation procedure in mice and ex vivo expanded in culture. IPCs were induced by transfection of pancreas and duodenal transcription factor 1 (PDX-1) mRNA in vitro. Some islet characteristics were identified on pMSC-derived IPCs in mRNA and protein levels. Our results demonstrated that mouse pMSCs can be transdifferentiated into effective glucose-responsive insulin-producing cells through transfecting synthetic modified PDX-1 mRNA in vitro. The study of PDX-1 mRNA-induced pMSC reprogramming may pave the way toward the development of a novel β-cell source for the treatment of diabetes.
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            Reprogramming
            PDX-1 mRNA
            Insulin-producing cells (IPCs)
            Internal Medicine
            Hematology
            Oncology
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      headline:PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro
      description: Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) from pancreas-derived mesenchymal stem cells (pMSCs). pMSCs were isolated from discarded pancreatic tissue in the filter liquor during islet isolation procedure in mice and ex vivo expanded in culture. IPCs were induced by transfection of pancreas and duodenal transcription factor 1 (PDX-1) mRNA in vitro. Some islet characteristics were identified on pMSC-derived IPCs in mRNA and protein levels. Our results demonstrated that mouse pMSCs can be transdifferentiated into effective glucose-responsive insulin-producing cells through transfecting synthetic modified PDX-1 mRNA in vitro. The study of PDX-1 mRNA-induced pMSC reprogramming may pave the way toward the development of a novel β-cell source for the treatment of diabetes.
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         Reprogramming
         PDX-1 mRNA
         Insulin-producing cells (IPCs)
         Internal Medicine
         Hematology
         Oncology
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