We are analyzing https://link.springer.com/article/10.1007/s10126-017-9777-1.

Title:
Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents | Marine Biotechnology
Description:
The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2–5 kg) much larger than average market size (0.6–1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer. Therefore, to overcome the obstacles of maturation size and generation time of tiger puffer, we generated surrogate grass puffer that can produce tiger puffer gametes through germ cell transplantation. Approximately 5000 tiger puffer testicular cells were transplanted into the peritoneal cavity of triploid grass puffer larvae at 1 day post hatching. When the recipient fish matured, both males and females produced donor-derived gametes. Through their insemination, we successfully produced donor-derived tiger puffer offspring presenting the same body surface dot pattern, number of dorsal fin rays, and DNA fingerprint as those of the donor tiger puffer, suggesting that the recipient grass puffer produced functional eggs and sperm derived from the donor tiger puffer. Although fine tunings are still needed to improve efficiencies, surrogate grass puffer are expected to accelerate the breeding process of tiger puffer because of their short generation time and small body size.
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Keywords {🔍}

article, google, scholar, pubmed, puffer, yoshizaki, cas, tiger, takeuchi, takifugu, germ, transplantation, rubripes, fish, production, cells, grass, reprod, biol, trout, offspring, triploid, yazawa, development, okutsu, maturation, cell, fugu, suzuki, central, shikina, generation, surrogate, donorderived, functional, eggs, access, genome, spermatogonial, spermatogonia, sci, privacy, cookies, content, information, marine, niphobles, hamasaki, miyaki, kikuchi,

Topics {✒️}

month download article/chapter donor-derived germ cells trans-species missense snp donor tiger puffer germ cell-deficient salmonids donor-derived offspring donor-derived gametes surrogate grass puffer triploid salmon parents testicular germ cells parental tiger puffer mature tiger puffer germ cells transplantation full article pdf spermatogonial stem cells ovarian germ cells germ cell transplantation male tiger pufferfish spermatogonial cell transplantation privacy choices/manage cookies produce functional eggs grass puffer germ-line chimeras tiger puffer author information authors recipient fish matured species-specific primers eggs derived generate functional gametes germ cells european economic area 1 day post hatching dorsal fin rays random amplicon sequencing southern bluefin tuna cold shock treatment flow-cytometric isolation light-scattering properties artificial hybrid pufferfishes trachurus japonicus article hamasaki conditions privacy policy small-bodied surrogate popular aquacultural fish average market size gonadotoropic hormone injections sex-determining locus lee kh small body size article log

Schema {🗺️}

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         headline:Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents
         description:The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2–5 kg) much larger than average market size (0.6–1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer. Therefore, to overcome the obstacles of maturation size and generation time of tiger puffer, we generated surrogate grass puffer that can produce tiger puffer gametes through germ cell transplantation. Approximately 5000 tiger puffer testicular cells were transplanted into the peritoneal cavity of triploid grass puffer larvae at 1 day post hatching. When the recipient fish matured, both males and females produced donor-derived gametes. Through their insemination, we successfully produced donor-derived tiger puffer offspring presenting the same body surface dot pattern, number of dorsal fin rays, and DNA fingerprint as those of the donor tiger puffer, suggesting that the recipient grass puffer produced functional eggs and sperm derived from the donor tiger puffer. Although fine tunings are still needed to improve efficiencies, surrogate grass puffer are expected to accelerate the breeding process of tiger puffer because of their short generation time and small body size.
         datePublished:2017-09-23T00:00:00Z
         dateModified:2017-09-23T00:00:00Z
         pageStart:579
         pageEnd:591
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            Triploid
            Grass puffer
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            Freshwater & Marine Ecology
            Microbiology
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            Engineering
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      headline:Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents
      description:The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2–5 kg) much larger than average market size (0.6–1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer. Therefore, to overcome the obstacles of maturation size and generation time of tiger puffer, we generated surrogate grass puffer that can produce tiger puffer gametes through germ cell transplantation. Approximately 5000 tiger puffer testicular cells were transplanted into the peritoneal cavity of triploid grass puffer larvae at 1 day post hatching. When the recipient fish matured, both males and females produced donor-derived gametes. Through their insemination, we successfully produced donor-derived tiger puffer offspring presenting the same body surface dot pattern, number of dorsal fin rays, and DNA fingerprint as those of the donor tiger puffer, suggesting that the recipient grass puffer produced functional eggs and sperm derived from the donor tiger puffer. Although fine tunings are still needed to improve efficiencies, surrogate grass puffer are expected to accelerate the breeding process of tiger puffer because of their short generation time and small body size.
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      dateModified:2017-09-23T00:00:00Z
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         Triploid
         Grass puffer
         Tiger puffer
         Freshwater & Marine Ecology
         Microbiology
         Zoology
         Engineering
         general
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            name:Kazushi Kadomura
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                  address:
                     name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
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            name:Toshiyuki Yamada
            affiliation:
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                  address:
                     name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
                     type:PostalAddress
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                  name:Nagasaki Prefectural Institute of Fisheries
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                     name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
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         type:PostalAddress
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         name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
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         type:PostalAddress
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         type:PostalAddress
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      name:Yutaka Takeuchi
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            name:Kagoshima University
            address:
               name:Division of Fisheries Resource and Sciences, Faculty of Fisheries, Kagoshima University, Kagoshima, Japan
               type:PostalAddress
            type:Organization
      name:Ryosuke Yazawa
      affiliation:
            name:Tokyo University of Marine Science and Technology
            address:
               name:Department Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan
               type:PostalAddress
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      name:Souta Yoshikawa
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               type:PostalAddress
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      name:Kazushi Kadomura
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            name:Nagasaki Prefectural Institute of Fisheries
            address:
               name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
               type:PostalAddress
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      name:Toshiyuki Yamada
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            name:Nagasaki Prefectural Institute of Fisheries
            address:
               name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
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            address:
               name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
               type:PostalAddress
            type:Organization
      name:Kiyoshi Kikuchi
      affiliation:
            name:University of Tokyo
            address:
               name:Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, University of Tokyo, Shizuoka, Japan
               type:PostalAddress
            type:Organization
      name:Goro Yoshizaki
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      name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
      name:Division of Fisheries Resource and Sciences, Faculty of Fisheries, Kagoshima University, Kagoshima, Japan
      name:Department Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan
      name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
      name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
      name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
      name:Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan
      name:Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, University of Tokyo, Shizuoka, Japan
      name:Department Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan
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