We are analyzing https://link.springer.com/article/10.1007/s00784-013-1088-y.

Title:
High-level β1-integrin expression in a subpopulation of highly tumorigenic oral cancer cells | Clinical Oral Investigations
Description:
Objectives The β1 integrin (CD29) is a putative marker for cancerous epithelial stem cells. Cancer stem cells are essential to drive tumor growth, recurrence, and metastasis. We investigated the role of β1-integrin expression in the development of malignant phenotypes of oral squamous cell carcinoma (OSCC). Materials and methods Immunostaining was used to analyze the expression levels of β1 integrins in different types of cell colonies and tumor spheres. The results of cell viability and migration assays with and without siRNA knockdown of β1-integrin expression were compared. Cells expressing β1 integrins were evaluated for their tumorigenicity in mice. The expression of β1 integrins in human specimens of oral cancers at different clinical stages was semiquantified based on immunohistochemical staining of the β1-integrin protein. Results The expression level of β1 integrins in Meng-1 oral epidermoid carcinoma cells (OECM-1) cells was significantly higher in holoclonal colonies and tumor spheres compared to control cells. The knockdown of β1-integrin expression in OECM-1 cells reduced cell proliferation, migration, and tumor sphere formation. Beta-1 integrin (+) cells were more tumorigenic in the mouse xenograft model than β1 integrin (−) cells. In the human specimens, the expression level of the β1-integrin protein positively correlated with the clinical stage. Conclusion The expression of β1 integrin in OECM-1 cells is involved in the development of malignant phenotypes of OSCC. Clinical relevance Inhibitors for β1-integrin signaling may be suitable to become target-specific therapies for OSCC.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We see no obvious way the site makes money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com might have a hidden revenue stream, but it's not something we can detect.

Keywords {🔍}

article, pubmed, cancer, oral, google, scholar, cell, cells, integrin, expression, stem, carcinoma, squamous, human, βintegrin, integrins, res, tumor, signaling, central, clinical, access, head, neck, clin, privacy, cookies, content, chen, yuan, growth, beta, national, taiwan, university, publish, search, lin, huang, wong, kuo, model, oncol, metastatic, springer, cheng, kung, author, analysis, data,

Topics {✒️}

tung-yiu wong & kuo yuan high-level β1-integrin expression chao-liang wu integrin β1/fak/akt signaling month download article/chapter potential therapeutic target carcinoma-derived cell lines metastasis-related gene expression beta1 integrin-dependent activation squamous cell carcinoma neck cancer cells cancer stem cell cancer stem cells cancer stem cells lymph node metastasis full article pdf oral verrucous carcinoma β1-integrin protein related subjects tumor dormancy induced stem cell patterning target-specific therapies β1-integrin expression β1-integrin signaling ovarian cancer cells privacy choices/manage cookies suppresses metastatic ability engineering integrin signaling oral epithelial progenitors mouse xenograft model jehn-shyun huang alpha1beta1 integrin inhibitor article lin mouse skin carcinogenesis experimental melanoma growth national science council author information authors endothelial cell migration kuo yuan de moraes rv de fougerolles ar drive tumor growth epithelial-mesenchymal transition altered expression linked de luca lm allosteric signaling machines yuh-ling chen cancer stem check access instant access

Schema {🗺️}

WebPage:
      mainEntity:
         headline:High-level β1-integrin expression in a subpopulation of highly tumorigenic oral cancer cells
         description:The β1 integrin (CD29) is a putative marker for cancerous epithelial stem cells. Cancer stem cells are essential to drive tumor growth, recurrence, and metastasis. We investigated the role of β1-integrin expression in the development of malignant phenotypes of oral squamous cell carcinoma (OSCC). Immunostaining was used to analyze the expression levels of β1 integrins in different types of cell colonies and tumor spheres. The results of cell viability and migration assays with and without siRNA knockdown of β1-integrin expression were compared. Cells expressing β1 integrins were evaluated for their tumorigenicity in mice. The expression of β1 integrins in human specimens of oral cancers at different clinical stages was semiquantified based on immunohistochemical staining of the β1-integrin protein. The expression level of β1 integrins in Meng-1 oral epidermoid carcinoma cells (OECM-1) cells was significantly higher in holoclonal colonies and tumor spheres compared to control cells. The knockdown of β1-integrin expression in OECM-1 cells reduced cell proliferation, migration, and tumor sphere formation. Beta-1 integrin (+) cells were more tumorigenic in the mouse xenograft model than β1 integrin (−) cells. In the human specimens, the expression level of the β1-integrin protein positively correlated with the clinical stage. The expression of β1 integrin in OECM-1 cells is involved in the development of malignant phenotypes of OSCC. Inhibitors for β1-integrin signaling may be suitable to become target-specific therapies for OSCC.
         datePublished:2013-08-25T00:00:00Z
         dateModified:2013-08-25T00:00:00Z
         pageStart:1277
         pageEnd:1284
         sameAs:https://doi.org/10.1007/s00784-013-1088-y
         keywords:
            Integrin
            Oral cancer
            Tumorigenesis
            Stem cells
            Dentistry
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig6_HTML.gif
         isPartOf:
            name:Clinical Oral Investigations
            issn:
               1436-3771
               1432-6981
            volumeNumber:18
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Berlin Heidelberg
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Hsiang-Chun Lin
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chao-Liang Wu
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yuh-Ling Chen
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jehn-Shyun Huang
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
                     name:National Cheng Kung University Hospital
                     address:
                        name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tung-Yiu Wong
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
                     name:National Cheng Kung University Hospital
                     address:
                        name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kuo Yuan
               affiliation:
                     name:National Cheng Kung University
                     address:
                        name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
                     name:National Cheng Kung University Hospital
                     address:
                        name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:High-level β1-integrin expression in a subpopulation of highly tumorigenic oral cancer cells
      description:The β1 integrin (CD29) is a putative marker for cancerous epithelial stem cells. Cancer stem cells are essential to drive tumor growth, recurrence, and metastasis. We investigated the role of β1-integrin expression in the development of malignant phenotypes of oral squamous cell carcinoma (OSCC). Immunostaining was used to analyze the expression levels of β1 integrins in different types of cell colonies and tumor spheres. The results of cell viability and migration assays with and without siRNA knockdown of β1-integrin expression were compared. Cells expressing β1 integrins were evaluated for their tumorigenicity in mice. The expression of β1 integrins in human specimens of oral cancers at different clinical stages was semiquantified based on immunohistochemical staining of the β1-integrin protein. The expression level of β1 integrins in Meng-1 oral epidermoid carcinoma cells (OECM-1) cells was significantly higher in holoclonal colonies and tumor spheres compared to control cells. The knockdown of β1-integrin expression in OECM-1 cells reduced cell proliferation, migration, and tumor sphere formation. Beta-1 integrin (+) cells were more tumorigenic in the mouse xenograft model than β1 integrin (−) cells. In the human specimens, the expression level of the β1-integrin protein positively correlated with the clinical stage. The expression of β1 integrin in OECM-1 cells is involved in the development of malignant phenotypes of OSCC. Inhibitors for β1-integrin signaling may be suitable to become target-specific therapies for OSCC.
      datePublished:2013-08-25T00:00:00Z
      dateModified:2013-08-25T00:00:00Z
      pageStart:1277
      pageEnd:1284
      sameAs:https://doi.org/10.1007/s00784-013-1088-y
      keywords:
         Integrin
         Oral cancer
         Tumorigenesis
         Stem cells
         Dentistry
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00784-013-1088-y/MediaObjects/784_2013_1088_Fig6_HTML.gif
      isPartOf:
         name:Clinical Oral Investigations
         issn:
            1436-3771
            1432-6981
         volumeNumber:18
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Berlin Heidelberg
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Hsiang-Chun Lin
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chao-Liang Wu
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yuh-Ling Chen
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jehn-Shyun Huang
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
                  name:National Cheng Kung University Hospital
                  address:
                     name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tung-Yiu Wong
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
                  name:National Cheng Kung University Hospital
                  address:
                     name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kuo Yuan
            affiliation:
                  name:National Cheng Kung University
                  address:
                     name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
                  name:National Cheng Kung University Hospital
                  address:
                     name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Clinical Oral Investigations
      issn:
         1436-3771
         1432-6981
      volumeNumber:18
Organization:
      name:Springer Berlin Heidelberg
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:National Cheng Kung University
      address:
         name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University
      address:
         name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University
      address:
         name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University
      address:
         name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University Hospital
      address:
         name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University
      address:
         name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University Hospital
      address:
         name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University
      address:
         name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
         type:PostalAddress
      name:National Cheng Kung University Hospital
      address:
         name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Hsiang-Chun Lin
      affiliation:
            name:National Cheng Kung University
            address:
               name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      name:Chao-Liang Wu
      affiliation:
            name:National Cheng Kung University
            address:
               name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      name:Yuh-Ling Chen
      affiliation:
            name:National Cheng Kung University
            address:
               name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      name:Jehn-Shyun Huang
      affiliation:
            name:National Cheng Kung University
            address:
               name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
            name:National Cheng Kung University Hospital
            address:
               name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      name:Tung-Yiu Wong
      affiliation:
            name:National Cheng Kung University
            address:
               name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
            name:National Cheng Kung University Hospital
            address:
               name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      name:Kuo Yuan
      affiliation:
            name:National Cheng Kung University
            address:
               name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
               type:PostalAddress
            type:Organization
            name:National Cheng Kung University Hospital
            address:
               name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
      name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
      name:Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      name:Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(143)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.41s.

LINK . SPRINGER . COM {}