We are analyzing https://link.springer.com/article/10.1007/s00726-008-0199-2.

Title:
Specificity of amino acid regulated gene expression: analysis of genes subjected to either complete or single amino acid deprivation | Amino Acids
Description:
Amino acid deprivation activates the amino acid response (AAR) pathway that enhances transcription of genes containing an amino acid response element (AARE). The present data reveal a quantitative difference in the response to deprivation of individual amino acids. The AAR leads to increased eukaryotic initiation factor 2α (eIF2α) phosphorylation and ATF4 translation. When HepG2 cells were deprived of an individual essential amino acid, p-eIF2α and activating transcription factor 4 were increased, but the correlation was relatively weak. Complete amino acid starvation in either Earle’s balanced salt solution or Krebs–Ringer bicarbonate buffer (KRB) resulted in activation of transcription driven by a SNAT2 genomic fragment that contained an AARE. However, for the KRB, a proportion of the transcription was AARE-independent suggesting that amino acid-independent mechanisms were responsible. Therefore, activation of AARE-driven transcription is triggered by a deficiency in any one of the essential amino acids, but the response is not uniform. Furthermore, caution must be exercised when using a medium completely devoid of amino acids.
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Keywords {🔍}

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Topics {✒️}

month download article/chapter amino acid-regulated transcription krebs–ringer bicarbonate buffer inra de clermont-theix substrate-induced adaptive regulation amino acid-independent mechanisms /ebp-atf composite site related subjects hepg2/c3a cells respond integrated stress response amino acid deprivation amino acid transport neutral amino acids full article pdf amino acid limitation slc38 gene family amino acid response amino acid sensing privacy choices/manage cookies amino acid availability human snat2 system amino acid transporters increased atf4 binding kimball sr endoplasmic reticulum stress balanced salt solution cap-independent translation individual amino acids human cell lines essential amino acids gene expression arginine/lysine transporter transporter gene acetylated amino acids snat2 genomic fragment unfolded protein response aare-driven transcription medium completely devoid mammalian glutamine transport translation reinitiation european economic area scope submit manuscript vander jagt dl localized histone acetylation dominy je jr oestrogen-primed uterus ross-inta cm threonine-devoid diet saint genès champanelle article palii

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         description:Amino acid deprivation activates the amino acid response (AAR) pathway that enhances transcription of genes containing an amino acid response element (AARE). The present data reveal a quantitative difference in the response to deprivation of individual amino acids. The AAR leads to increased eukaryotic initiation factor 2α (eIF2α) phosphorylation and ATF4 translation. When HepG2 cells were deprived of an individual essential amino acid, p-eIF2α and activating transcription factor 4 were increased, but the correlation was relatively weak. Complete amino acid starvation in either Earle’s balanced salt solution or Krebs–Ringer bicarbonate buffer (KRB) resulted in activation of transcription driven by a SNAT2 genomic fragment that contained an AARE. However, for the KRB, a proportion of the transcription was AARE-independent suggesting that amino acid-independent mechanisms were responsible. Therefore, activation of AARE-driven transcription is triggered by a deficiency in any one of the essential amino acids, but the response is not uniform. Furthermore, caution must be exercised when using a medium completely devoid of amino acids.
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