We are analyzing https://link.springer.com/article/10.1007/s007090200018.

Title:
Jasplakinolide reversibly disrupts actin filaments in suspension-cultured tobacco BY-2 cells | Protoplasma
Description:
Jasplakinolide is potentially a useful pharmacological tool for the study of actin organization and dynamics in living cells, since it induces actin polymerization in vitro and, unlike phalloidin, is membrane permeative. In the present work, the effect of jasplakinolide on the actin cytoskeleton of living suspension-cultured Nicotiana tabacum ‘Bright Yellow 2’ cells was investigated. Actin filaments in the living cells were disrupted by jasplakinolide. The effect of jasplakionlide on the actin cytoskeleton was concentration and time dependent. When cells were treated with a moderate concentration (150 nM) of jasplakinolide, cortical actin filaments were disrupted preferentially, whereas actin aggregated at the perinuclear region. With concentrations higher than 400 nM and exposure times longer than 30 min, actin filaments in the cell disappeared completely. The effect of jasplakinolide on the actin cytoskeleton was reversible even at high concentration. Actin bundles appeared first in the perinuclear region within 5 min, and the cortical actin array was reestablished in 15 min, suggesting that actin filaments might be organized at this region.
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Keywords {🔍}

actin, jasplakinolide, article, cells, filaments, plant, privacy, cookies, content, cytoskeleton, access, information, publish, search, data, log, journal, research, protoplasma, suspensioncultured, chen, yuan, living, phalloidin, effect, concentration, region, min, chapter, discover, springer, function, optional, personal, parties, policy, find, track, reversibly, disrupts, tobacco, cite, explore, dynamics, nicotiana, tabacum, bright, yellow, disrupted, cortical,

Topics {✒️}

month download article/chapter nicotiana tabacum var cell disappeared completely article protoplasma aims suspension-cultured tobacco related subjects privacy choices/manage cookies full article pdf cortical actin filaments bright yellow 2 induces actin polymerization actin bundles appeared cortical actin array actin-perturbing substances european economic area scope submit manuscript exposure times longer china agricultural university conditions privacy policy actin cytoskeleton accepting optional cookies biological sciences actin filaments journal finder publish plant cells article log plant physiology plant sciences living cells check access instant access 1007/s007090200018 keywords article cite actin dynamics article ou privacy policy personal data books a actin organization actin aggregated actin filament optional cookies manage preferences protoplasma 219 subscription content similar content data protection essential cookies cookies skip 2 cells published

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