We are analyzing https://link.springer.com/article/10.1007/s00441-006-0260-y.

Title:
Identification and expression analysis of rainbow trout pumilio-1 and pumilio-2 | Cell and Tissue Research
Description:
Pumilio is a sequence-specific RNA-binding protein that regulates translation from the relevant mRNA. The PUF-domain, the RNA-binding motif of Pumilio, is highly conserved across species. In the present study, we have identified two pumilio genes (pumilio-1 and pumilio-2) in rainbow trout and analyzed their expression patterns in its tissues. Pumilio-1 mRNA and pumilio-2A mRNA code for typical full length Pumilio proteins that contain a PUF-domain, whereas pumilio-2B mRNA is a splice variant of pumilio-2 and encodes a protein that lacks the PUF-domain. We have also identified a novel 72-bp exon that has not been reported in other animal species but is conserved in fish species. The insertion of this novel exon leads to the expression of an isoform of the Pumilio-2 protein with a slightly altered conformation of the PUF-domain. Pumilio-1 mRNA and pumilio-2A mRNA (irrespective of the presence of the 72-bp exon) are expressed in both the brain and ovaries at high levels, whereas pumilio-2B mRNA is expressed at low levels in all the rainbow trout tissues examined. Western blot analysis also indicates that the full length Pumilio proteins are expressed predominantly in the brain and ovaries. These data suggest that the Pumilio proteins have physiological roles and are involved in regulatory mechanisms in rainbow trout.
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Keywords {🔍}

article, cas, google, scholar, pubmed, pumilio, mrna, proteins, cell, rnabinding, protein, rna, rainbow, trout, conserved, research, access, puf, biol, ito, drosophila, privacy, cookies, content, data, identification, yamashita, etsuro, pufdomain, brain, res, human, dev, mol, analysis, information, publish, search, expression, matsuoka, translation, expressed, open, binding, domain, splicing, usa, curr, urano, reijo,

Topics {✒️}

sequence-specific rna-binding protein cell-specific rna-binding proteins rna-binding protein musashi-1 conserved rna-binding protein month download article/chapter ddbj/embl/genbank databases drosophila long-term memory alternative splicing pumilio-2a mrna code human pumilio-homology domain pre-mrna splicing cyclin b1 mrna full article pdf puf-rna binding rna-binding proteins rna-binding motif related subjects tail-dependent translation privacy choices/manage cookies rainbow trout pumilio-1 pumilio homology domain nucleotide sequence data tissue research aims mrna surveillance machinery regulates sexual fates pumilio-2a mrna kinesin transports rna rna-transporting granule exon-exon junctions drosophila germ plasm drosophila peripheral neurons pumilio-2b mrna conserved meiotic regulators mrna regulatory proteins pumilio-nanos complex staufen/pumilio pathway rna binding article kurisaki european economic area slightly altered conformation comprehensive thermodynamic model single-step method reijo pera ra embryonic stem cells alpha-actinin transcript promoting posterior deadenylation regulates translation conditions privacy policy article cell etsuro ito

Questions {❓}

Spassov DS, Jurecic R (2003) The PUF family of RNA-binding proteins: does evolutionarily conserved structure equal conserved function?

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         description:Pumilio is a sequence-specific RNA-binding protein that regulates translation from the relevant mRNA. The PUF-domain, the RNA-binding motif of Pumilio, is highly conserved across species. In the present study, we have identified two pumilio genes (pumilio-1 and pumilio-2) in rainbow trout and analyzed their expression patterns in its tissues. Pumilio-1 mRNA and pumilio-2A mRNA code for typical full length Pumilio proteins that contain a PUF-domain, whereas pumilio-2B mRNA is a splice variant of pumilio-2 and encodes a protein that lacks the PUF-domain. We have also identified a novel 72-bp exon that has not been reported in other animal species but is conserved in fish species. The insertion of this novel exon leads to the expression of an isoform of the Pumilio-2 protein with a slightly altered conformation of the PUF-domain. Pumilio-1 mRNA and pumilio-2A mRNA (irrespective of the presence of the 72-bp exon) are expressed in both the brain and ovaries at high levels, whereas pumilio-2B mRNA is expressed at low levels in all the rainbow trout tissues examined. Western blot analysis also indicates that the full length Pumilio proteins are expressed predominantly in the brain and ovaries. These data suggest that the Pumilio proteins have physiological roles and are involved in regulatory mechanisms in rainbow trout.
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            RNA-binding protein
            PUF-domain
            Alternative splicing
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             Oncorhynchus mykiss (Teleostei)
            Human Genetics
            Proteomics
            Molecular Medicine
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      headline:Identification and expression analysis of rainbow trout pumilio-1 and pumilio-2
      description:Pumilio is a sequence-specific RNA-binding protein that regulates translation from the relevant mRNA. The PUF-domain, the RNA-binding motif of Pumilio, is highly conserved across species. In the present study, we have identified two pumilio genes (pumilio-1 and pumilio-2) in rainbow trout and analyzed their expression patterns in its tissues. Pumilio-1 mRNA and pumilio-2A mRNA code for typical full length Pumilio proteins that contain a PUF-domain, whereas pumilio-2B mRNA is a splice variant of pumilio-2 and encodes a protein that lacks the PUF-domain. We have also identified a novel 72-bp exon that has not been reported in other animal species but is conserved in fish species. The insertion of this novel exon leads to the expression of an isoform of the Pumilio-2 protein with a slightly altered conformation of the PUF-domain. Pumilio-1 mRNA and pumilio-2A mRNA (irrespective of the presence of the 72-bp exon) are expressed in both the brain and ovaries at high levels, whereas pumilio-2B mRNA is expressed at low levels in all the rainbow trout tissues examined. Western blot analysis also indicates that the full length Pumilio proteins are expressed predominantly in the brain and ovaries. These data suggest that the Pumilio proteins have physiological roles and are involved in regulatory mechanisms in rainbow trout.
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         Alternative splicing
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         Human Genetics
         Proteomics
         Molecular Medicine
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