We are analyzing https://link.springer.com/article/10.1007/s00441-005-0140-x.

Title:
Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine | Cell and Tissue Research
Description:
Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are the major anions in the large intestinal lumen. They are produced from dietary fiber by bacterial fermentation and are known to have a variety of physiological and pathophysiological effects on the intestine. In the present study, we investigated the expression of the SCFA receptor, GPR43, in the rat distal ileum and colon. Expression of GPR43 was detected by reverse transcriptase/polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. mRNA for GPR43 was detected, by RT-PCR, in extracts of the whole wall and separated mucosa from the ileum and colon and from muscle plus submucosa from the ileum, but not from muscle plus submucosa preparations from the colon. We raised a rabbit antiserum against a synthesized fragment of rat GPR43; this was specific for rat GPR43. GPR43 protein was detected by Western blot analysis in extracts of whole wall and separated mucosa, but not in muscle plus submucosa extracts. By immunohistochemistry, GPR43 immunoreactivity was localized to enteroendocrine cells expressing peptide YY (PYY), whereas 5-hydroxytryptamine (5-HT)-immunoreactive (IR) enteroendocrine cells were not immunoreactive for GPR43. Mast cells of the lamina propria expressing 5-HT were also GPR43-IR. The results of the present study suggest that the PYY-containing enteroendocrine cells and 5-HT-containing mucosal mast cells sense SCFAs via the GPR43 receptor. This is consistent with physiological data showing that SCFAs stimulate the release of PYY and 5-HT from the ileum and colon.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

article, google, scholar, cas, pubmed, fatty, gpr, physiol, rat, shortchain, acids, cells, colon, research, cell, enteroendocrine, intestine, peptide, access, gastrointestinal, university, privacy, cookies, content, mucosal, mast, karaki, mitsui, kuwahara, intestinal, distal, pyy, receptors, author, japan, data, information, publish, search, acid, receptor, hayashi, effects, expression, ileum, muscle, release, motility, lin, depends,

Topics {✒️}

protein–coupled receptors gpr41 short–chain fatty acids month download article/chapter mucosal mast cells fat–induced ileal brake propionate–induced contractile responses fatty acids full article pdf polymorphonuclear cell activation article cell tissue research aims peptide yy depends human receptors retsu mitsui rat gastrointestinal tracts hisayoshi hayashi privacy choices/manage cookies organic acid absorption gastrointestinal endocrine cells large intestinal lumen slowing intestinal transit intestinal blood flow australian research council related subjects bilchik aj author correspondence astrazeneca research grant polypeptide yy release check access instant access human gastrointestinal tract encephalitogenic t-cells rat intestine peptide yy mast cells gastrointestinal motility rat distal colon european economic area central nervous system intestinal transit epithelial chloride secretion cambridge university press article karaki conditions privacy policy sodium oleate depends beta–adrenergic pathway gpr43 protein rat distal ileum enteroendocrine cells article log

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine
         description:Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are the major anions in the large intestinal lumen. They are produced from dietary fiber by bacterial fermentation and are known to have a variety of physiological and pathophysiological effects on the intestine. In the present study, we investigated the expression of the SCFA receptor, GPR43, in the rat distal ileum and colon. Expression of GPR43 was detected by reverse transcriptase/polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. mRNA for GPR43 was detected, by RT-PCR, in extracts of the whole wall and separated mucosa from the ileum and colon and from muscle plus submucosa from the ileum, but not from muscle plus submucosa preparations from the colon. We raised a rabbit antiserum against a synthesized fragment of rat GPR43; this was specific for rat GPR43. GPR43 protein was detected by Western blot analysis in extracts of whole wall and separated mucosa, but not in muscle plus submucosa extracts. By immunohistochemistry, GPR43 immunoreactivity was localized to enteroendocrine cells expressing peptide YY (PYY), whereas 5-hydroxytryptamine (5-HT)-immunoreactive (IR) enteroendocrine cells were not immunoreactive for GPR43. Mast cells of the lamina propria expressing 5-HT were also GPR43-IR. The results of the present study suggest that the PYY-containing enteroendocrine cells and 5-HT-containing mucosal mast cells sense SCFAs via the GPR43 receptor. This is consistent with physiological data showing that SCFAs stimulate the release of PYY and 5-HT from the ileum and colon.
         datePublished:2006-02-01T00:00:00Z
         dateModified:2006-02-01T00:00:00Z
         pageStart:353
         pageEnd:360
         sameAs:https://doi.org/10.1007/s00441-005-0140-x
         keywords:
            Short-chain fatty acids
            GPR43
            Enteroendocrine cell
            Mucosal mast cell
            Peptide YY
            5-Hydroxytryptamine
            Intestine
            Rat (Wistar, male)
            Human Genetics
            Proteomics
            Molecular Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig6_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig7_HTML.gif
         isPartOf:
            name:Cell and Tissue Research
            issn:
               1432-0878
               0302-766X
            volumeNumber:324
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Shin-ichiro Karaki
               affiliation:
                     name:University of Shizuoka
                     address:
                        name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Retsu Mitsui
               affiliation:
                     name:University of Shizuoka
                     address:
                        name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hisayoshi Hayashi
               affiliation:
                     name:University of Shizuoka
                     address:
                        name:Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ikuo Kato
               affiliation:
                     name:Yanaihara Institute
                     address:
                        name:Yanaihara Institute, Fujinomiya-shi, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hiroshi Sugiya
               affiliation:
                     name:Nihon University School of Dentistry at Matsudo
                     address:
                        name:Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Toshihiko Iwanaga
               affiliation:
                     name:Hokkaido University
                     address:
                        name:Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:John B. Furness
               affiliation:
                     name:University of Melbourne
                     address:
                        name:Department of Anatomy & Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Atsukazu Kuwahara
               affiliation:
                     name:University of Shizuoka
                     address:
                        name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine
      description:Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are the major anions in the large intestinal lumen. They are produced from dietary fiber by bacterial fermentation and are known to have a variety of physiological and pathophysiological effects on the intestine. In the present study, we investigated the expression of the SCFA receptor, GPR43, in the rat distal ileum and colon. Expression of GPR43 was detected by reverse transcriptase/polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. mRNA for GPR43 was detected, by RT-PCR, in extracts of the whole wall and separated mucosa from the ileum and colon and from muscle plus submucosa from the ileum, but not from muscle plus submucosa preparations from the colon. We raised a rabbit antiserum against a synthesized fragment of rat GPR43; this was specific for rat GPR43. GPR43 protein was detected by Western blot analysis in extracts of whole wall and separated mucosa, but not in muscle plus submucosa extracts. By immunohistochemistry, GPR43 immunoreactivity was localized to enteroendocrine cells expressing peptide YY (PYY), whereas 5-hydroxytryptamine (5-HT)-immunoreactive (IR) enteroendocrine cells were not immunoreactive for GPR43. Mast cells of the lamina propria expressing 5-HT were also GPR43-IR. The results of the present study suggest that the PYY-containing enteroendocrine cells and 5-HT-containing mucosal mast cells sense SCFAs via the GPR43 receptor. This is consistent with physiological data showing that SCFAs stimulate the release of PYY and 5-HT from the ileum and colon.
      datePublished:2006-02-01T00:00:00Z
      dateModified:2006-02-01T00:00:00Z
      pageStart:353
      pageEnd:360
      sameAs:https://doi.org/10.1007/s00441-005-0140-x
      keywords:
         Short-chain fatty acids
         GPR43
         Enteroendocrine cell
         Mucosal mast cell
         Peptide YY
         5-Hydroxytryptamine
         Intestine
         Rat (Wistar, male)
         Human Genetics
         Proteomics
         Molecular Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig6_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-005-0140-x/MediaObjects/441_2005_140_Fig7_HTML.gif
      isPartOf:
         name:Cell and Tissue Research
         issn:
            1432-0878
            0302-766X
         volumeNumber:324
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Shin-ichiro Karaki
            affiliation:
                  name:University of Shizuoka
                  address:
                     name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Retsu Mitsui
            affiliation:
                  name:University of Shizuoka
                  address:
                     name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hisayoshi Hayashi
            affiliation:
                  name:University of Shizuoka
                  address:
                     name:Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ikuo Kato
            affiliation:
                  name:Yanaihara Institute
                  address:
                     name:Yanaihara Institute, Fujinomiya-shi, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hiroshi Sugiya
            affiliation:
                  name:Nihon University School of Dentistry at Matsudo
                  address:
                     name:Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Toshihiko Iwanaga
            affiliation:
                  name:Hokkaido University
                  address:
                     name:Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:John B. Furness
            affiliation:
                  name:University of Melbourne
                  address:
                     name:Department of Anatomy & Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Atsukazu Kuwahara
            affiliation:
                  name:University of Shizuoka
                  address:
                     name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cell and Tissue Research
      issn:
         1432-0878
         0302-766X
      volumeNumber:324
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Shizuoka
      address:
         name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
         type:PostalAddress
      name:University of Shizuoka
      address:
         name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
         type:PostalAddress
      name:University of Shizuoka
      address:
         name:Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
         type:PostalAddress
      name:Yanaihara Institute
      address:
         name:Yanaihara Institute, Fujinomiya-shi, Japan
         type:PostalAddress
      name:Nihon University School of Dentistry at Matsudo
      address:
         name:Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:University of Melbourne
      address:
         name:Department of Anatomy & Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, Australia
         type:PostalAddress
      name:University of Shizuoka
      address:
         name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Shin-ichiro Karaki
      affiliation:
            name:University of Shizuoka
            address:
               name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
               type:PostalAddress
            type:Organization
      name:Retsu Mitsui
      affiliation:
            name:University of Shizuoka
            address:
               name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
               type:PostalAddress
            type:Organization
      name:Hisayoshi Hayashi
      affiliation:
            name:University of Shizuoka
            address:
               name:Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
               type:PostalAddress
            type:Organization
      name:Ikuo Kato
      affiliation:
            name:Yanaihara Institute
            address:
               name:Yanaihara Institute, Fujinomiya-shi, Japan
               type:PostalAddress
            type:Organization
      name:Hiroshi Sugiya
      affiliation:
            name:Nihon University School of Dentistry at Matsudo
            address:
               name:Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
               type:PostalAddress
            type:Organization
      name:Toshihiko Iwanaga
      affiliation:
            name:Hokkaido University
            address:
               name:Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:John B. Furness
      affiliation:
            name:University of Melbourne
            address:
               name:Department of Anatomy & Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, Australia
               type:PostalAddress
            type:Organization
      name:Atsukazu Kuwahara
      affiliation:
            name:University of Shizuoka
            address:
               name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
      name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
      name:Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
      name:Yanaihara Institute, Fujinomiya-shi, Japan
      name:Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
      name:Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
      name:Department of Anatomy & Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, Australia
      name:Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(92)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.99s.

LINK . SPRINGER . COM {}