We are analyzing https://link.springer.com/article/10.1007/s00441-004-1068-2.

Title:
Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor | Cell and Tissue Research
Description:
The effects of vascular factors on the nervous system are still poorly investigated. Angiopoietin-1 (Ang-1), an endothelial cell growth factor with influences on blood vessel stabilization, has been recently reported to prevent apoptosis in a neuroblastoma cell line via a pathway dependent on Tie-2 receptor. The present study focuses on the effect of Ang-1 on cultured dorsal root ganglion (DRG) cells isolated from 1-day-old rats. Three-day-old DRG cultures were exposed to Ang-1 treatment under serum-free condition for another 5 days and stained with antibodies against neurofilament (NF) 200 protein. Neurite length and density increased compared with those of controls. Double-immunofluorescence staining demonstrated the co-localization of the Tie-2 receptor in some NF-200-positive perikarya. The reverse transcription/polymerase chain reaction technique identified Tie-2 receptor mRNA in intact DRG and in Ang-1-stimulated DRG cell cultures, but not in a Schwann cell line or in primary astrocyte cultures. Western blotting confirmed that the expression of NF 68 protein in cultures treated with Ang-1 or nerve growth factor was higher than that in cultures treated with medium alone. When the Tie-2 receptor was blocked with anti-Tie-2 receptor antibody, neurite outgrowth was severely impeded. Induction of trkA-receptor protein expression was observed to be dependent on the presence of Tie-2 receptors. We conclude that Ang-1 promotes neurite outgrowth from DRG cells positive for Tie-2 receptor. The signalling pathway appears to involve transactivation of the trkA receptor.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure how the site profits.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

google, scholar, cell, receptor, article, tie, growth, factor, cells, dorsal, root, outgrowth, ganglion, expression, cas, pubmed, res, neurite, endothelial, role, privacy, cookies, content, research, tissue, angiopoietin, engele, factors, ang, drg, cultures, access, nature, neurosci, neurotrophin, information, publish, search, positive, figiel, spanelborowski, nervous, system, protein, nerve, receptors, mice, sensory, survival, angiogenesis,

Topics {✒️}

protein-coupled receptors month download article/chapter vascular-specific growth factors trka-receptor protein expression dorsal root ganglion hematopoietic stem cells anti-tie-2 receptor antibody post-ganglionic sympathetic neurons double-immunofluorescence staining demonstrated brain-derived neurotophic factor katharina spanel-borowski human tie2-agonistic antibody signalling pathway appears fibroblast growth factors nerve growth factor stimulates axonal outgrowth tissue research aims tie-2 receptors angiopoietin/tie pathway drg cells positive angiopoietin-1-mediated migration full article pdf ear sensory neurons trka receptor privacy choices/manage cookies schwann cell line schwann cell proliferation autocrine motility factor neurite outgrowth endothelial cell influence sensory neuron subpopulation mice overexpressing angiopoietin-1 blood vessel formation related subjects spanel-borowski endothelial cells vascular factors normal synovial tissue p75 neurotrophin receptor neuroblastoma cell line enhancing cell survival blood vessel stabilization rat hepatocarcinogenesis acellular nerve grafts european economic area serum-free condition western blotting confirmed improves penile neurovasculature cerebral ischemia-reperfusion neurotrophin signal transduction

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor
         description:The effects of vascular factors on the nervous system are still poorly investigated. Angiopoietin-1 (Ang-1), an endothelial cell growth factor with influences on blood vessel stabilization, has been recently reported to prevent apoptosis in a neuroblastoma cell line via a pathway dependent on Tie-2 receptor. The present study focuses on the effect of Ang-1 on cultured dorsal root ganglion (DRG) cells isolated from 1-day-old rats. Three-day-old DRG cultures were exposed to Ang-1 treatment under serum-free condition for another 5 days and stained with antibodies against neurofilament (NF) 200 protein. Neurite length and density increased compared with those of controls. Double-immunofluorescence staining demonstrated the co-localization of the Tie-2 receptor in some NF-200-positive perikarya. The reverse transcription/polymerase chain reaction technique identified Tie-2 receptor mRNA in intact DRG and in Ang-1-stimulated DRG cell cultures, but not in a Schwann cell line or in primary astrocyte cultures. Western blotting confirmed that the expression of NF 68 protein in cultures treated with Ang-1 or nerve growth factor was higher than that in cultures treated with medium alone. When the Tie-2 receptor was blocked with anti-Tie-2 receptor antibody, neurite outgrowth was severely impeded. Induction of trkA-receptor protein expression was observed to be dependent on the presence of Tie-2 receptors. We conclude that Ang-1 promotes neurite outgrowth from DRG cells positive for Tie-2 receptor. The signalling pathway appears to involve transactivation of the trkA receptor.
         datePublished:2005-02-16T00:00:00Z
         dateModified:2005-02-16T00:00:00Z
         pageStart:11
         pageEnd:19
         sameAs:https://doi.org/10.1007/s00441-004-1068-2
         keywords:
            Angiopoietin-1
            Tie-2 receptor
            trkA receptor
            Neurite outgrowth
            Dorsal root ganglion cells
            Rat (Wistar-Furt)
            Human Genetics
            Proteomics
            Molecular Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb1.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb2.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb3.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb4.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb5.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb6.tif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb7.tif
         isPartOf:
            name:Cell and Tissue Research
            issn:
               1432-0878
               0302-766X
            volumeNumber:320
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Joanna Kosacka
               affiliation:
                     name:University of Leipzig
                     address:
                        name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Maciej Figiel
               affiliation:
                     name:University of Leipzig
                     address:
                        name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jürgen Engele
               affiliation:
                     name:University of Leipzig
                     address:
                        name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Heidegard Hilbig
               affiliation:
                     name:University of Leipzig
                     address:
                        name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mariusz Majewski
               affiliation:
                     name:University Warmia and Mazury
                     address:
                        name:Faculty of Veterinary Medicine, University Warmia and Mazury, Olsztyn, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Katharina Spanel-Borowski
               affiliation:
                     name:University of Leipzig
                     address:
                        name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor
      description:The effects of vascular factors on the nervous system are still poorly investigated. Angiopoietin-1 (Ang-1), an endothelial cell growth factor with influences on blood vessel stabilization, has been recently reported to prevent apoptosis in a neuroblastoma cell line via a pathway dependent on Tie-2 receptor. The present study focuses on the effect of Ang-1 on cultured dorsal root ganglion (DRG) cells isolated from 1-day-old rats. Three-day-old DRG cultures were exposed to Ang-1 treatment under serum-free condition for another 5 days and stained with antibodies against neurofilament (NF) 200 protein. Neurite length and density increased compared with those of controls. Double-immunofluorescence staining demonstrated the co-localization of the Tie-2 receptor in some NF-200-positive perikarya. The reverse transcription/polymerase chain reaction technique identified Tie-2 receptor mRNA in intact DRG and in Ang-1-stimulated DRG cell cultures, but not in a Schwann cell line or in primary astrocyte cultures. Western blotting confirmed that the expression of NF 68 protein in cultures treated with Ang-1 or nerve growth factor was higher than that in cultures treated with medium alone. When the Tie-2 receptor was blocked with anti-Tie-2 receptor antibody, neurite outgrowth was severely impeded. Induction of trkA-receptor protein expression was observed to be dependent on the presence of Tie-2 receptors. We conclude that Ang-1 promotes neurite outgrowth from DRG cells positive for Tie-2 receptor. The signalling pathway appears to involve transactivation of the trkA receptor.
      datePublished:2005-02-16T00:00:00Z
      dateModified:2005-02-16T00:00:00Z
      pageStart:11
      pageEnd:19
      sameAs:https://doi.org/10.1007/s00441-004-1068-2
      keywords:
         Angiopoietin-1
         Tie-2 receptor
         trkA receptor
         Neurite outgrowth
         Dorsal root ganglion cells
         Rat (Wistar-Furt)
         Human Genetics
         Proteomics
         Molecular Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb1.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb2.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb3.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb4.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb5.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fhb6.tif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-004-1068-2/MediaObjects/s00441-004-1068-2fmb7.tif
      isPartOf:
         name:Cell and Tissue Research
         issn:
            1432-0878
            0302-766X
         volumeNumber:320
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Joanna Kosacka
            affiliation:
                  name:University of Leipzig
                  address:
                     name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Maciej Figiel
            affiliation:
                  name:University of Leipzig
                  address:
                     name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jürgen Engele
            affiliation:
                  name:University of Leipzig
                  address:
                     name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Heidegard Hilbig
            affiliation:
                  name:University of Leipzig
                  address:
                     name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mariusz Majewski
            affiliation:
                  name:University Warmia and Mazury
                  address:
                     name:Faculty of Veterinary Medicine, University Warmia and Mazury, Olsztyn, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Katharina Spanel-Borowski
            affiliation:
                  name:University of Leipzig
                  address:
                     name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cell and Tissue Research
      issn:
         1432-0878
         0302-766X
      volumeNumber:320
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Leipzig
      address:
         name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
         type:PostalAddress
      name:University of Leipzig
      address:
         name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
         type:PostalAddress
      name:University of Leipzig
      address:
         name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
         type:PostalAddress
      name:University of Leipzig
      address:
         name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
         type:PostalAddress
      name:University Warmia and Mazury
      address:
         name:Faculty of Veterinary Medicine, University Warmia and Mazury, Olsztyn, Poland
         type:PostalAddress
      name:University of Leipzig
      address:
         name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Joanna Kosacka
      affiliation:
            name:University of Leipzig
            address:
               name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Maciej Figiel
      affiliation:
            name:University of Leipzig
            address:
               name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Jürgen Engele
      affiliation:
            name:University of Leipzig
            address:
               name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Heidegard Hilbig
      affiliation:
            name:University of Leipzig
            address:
               name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Mariusz Majewski
      affiliation:
            name:University Warmia and Mazury
            address:
               name:Faculty of Veterinary Medicine, University Warmia and Mazury, Olsztyn, Poland
               type:PostalAddress
            type:Organization
      name:Katharina Spanel-Borowski
      affiliation:
            name:University of Leipzig
            address:
               name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
      name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
      name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
      name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
      name:Faculty of Veterinary Medicine, University Warmia and Mazury, Olsztyn, Poland
      name:Institute of Anatomy, University of Leipzig, Leipzig, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(82)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.74s.

LINK . SPRINGER . COM {}