We are analyzing https://link.springer.com/article/10.1007/s00441-002-0562-7.

Title:
Redifferentiation of dedifferentiated human chondrocytes in high-density cultures | Cell and Tissue Research
Description:
High-density cultures are widely used as an in vitro model for studies of embryonic cartilage formation. In the present study we investigated the suitability of high-density cultures for the redifferentiation of dedifferentiated chondrocytes. When primary human chondrocytes were cultured in alginate beads, some cells emigrated into Petri dishes. These cells were cultured for one to eight passages (each passage lasting about 3 days) in monolayer culture. At each passage, monolayer cells were removed and allowed to grow in high-density cultures at the medium-air interface and subsequently investigated with morphological, immunolocalization and biochemical methods for the production of cartilage-specific markers, i.e. collagen type II and cartilage-specific proteoglycans. When such dedifferentiated chondrocytes from monolayer passages P1–P4 were introduced in high-density culture, they regained a chondrocyte phenotype and formed cartilage nodules surrounded by fibroblast-like cells. Cells were interconnected by typical gap junctions and after a few days in culturing produced cartilage-specific extracellular matrix, notably collagen type II and cartilage-specific proteoglycans. In contrast, cells taken from monolayer passages P5–P8 did not produce these chondrocyte-specific extracellular materials when grown in high-density culture. We conclude that the growth of dedifferentiated chondrocytes in high-density culture promotes their redifferentiation and reveals their chondrogenic potential. Such high-density cultures might serve as a model system to initiate and promote the redifferentiation of chondrocytes and to provide sufficient quantities of differentiated chondrocytes for autologous chondrocyte transplantation.
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Keywords {🔍}

article, chondrocytes, highdensity, cultures, culture, cell, redifferentiation, dedifferentiated, human, cells, privacy, cookies, content, research, tissue, access, information, publish, search, cartilage, monolayer, cartilagespecific, chondrocyte, berlin, data, log, journal, schulzetanzil, souza, villegas, castrejon, primary, passages, collagen, type, discover, springer, optional, personal, parties, policy, find, track, june, cite, john, merker, scheid, shakibaei, explore,

Topics {✒️}

collagen type ii high-density culture promotes chondrocyte-specific extracellular materials month download article/chapter monolayer passages p1–p4 monolayer passages p5–p8 high-density culture primary chondrocyte culture high-density cultures tissue research aims primary human chondrocytes cartilage-specific markers cartilage-specific proteoglycans privacy choices/manage cookies article cell autologous chondrocyte transplantation full article pdf embryonic cartilage formation dedifferentiated human chondrocytes human chondrogenic phenotype european economic area scope submit manuscript medium-air interface typical gap junctions provide sufficient quantities dendrimer-immobilized surface cadaveric donor enhance königin-luise-strasse 15 university childrens' hospital article schulze-tanzil conditions privacy policy monolayer culture accepting optional cookies check access freie universität berlin instant access main content log journal finder publish chondrocyte phenotype repair tissue article log june 2002 volume 308 present study related subjects privacy policy personal data dedifferentiated chondrocytes article cite differentiated chondrocytes books a

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