We are analyzing https://link.springer.com/article/10.1007/s00438-002-0751-9.

Title:
Renilla luciferase-Aequorea GFP (Ruc-GFP) fusion protein, a novel dual reporter for real-time imaging of gene expression in cell cultures and in live animals | Molecular Genetics and Genomics
Description:
Light-emitting reporter proteins play an increasing role in the study of gene expression in vitro and in vivo. Here we present a ruc-gfp fusion gene construct generated by fusing a cDNA for Renilla luciferase (ruc) in-frame with a cDNA encoding the
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Telecommunications
Mobile Technology & AI

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Earning money isn't the goal of every website; some are designed to offer support or promote social causes. People have different reasons for creating websites. This might be one such reason. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

gene, article, gfp, fusion, renilla, rucgfp, luciferase, protein, fluorescence, reporter, access, privacy, cookies, content, wang, cells, information, publish, search, imaging, expression, aequorea, emission, data, log, journal, research, genomics, live, animals, shabahang, construct, transformed, presence, coelenterazine, open, discover, springer, optional, analysis, personal, parties, policy, find, track, molecular, genetics, luciferaseaequorea, dual, realtime,

Topics {✒️}

month download article/chapter renilla luciferase-aequorea gfp c-terminal lysine residue ruc-gfp fusion protein ruc-gfp fusion gene real-time imaging exciting gfp fluorescence article molecular genetics single-cell level based fusion gene construct privacy choices/manage cookies renilla luciferase activity related subjects full article pdf gfp fluorescence green fluorescence live animals bearing european economic area scope submit manuscript intramolecular energy transfer genomic dna purified metaphase chromosomes confirmed animal experiments revealed high-throughput screening conditions privacy policy fusion protein dual reporter accepting optional cookies aequorea gfp renilla luciferase loma linda university gene construct transformed cells exhibited october 2002 volume 268 journal finder publish ruc-gfp fluorescence live animals check access instant access article log gene expression luciferase substrate information privacy policy personal data genomics aims 'humanized' gfp article cite books a

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Renilla luciferase-Aequorea GFP (Ruc-GFP) fusion protein, a novel dual reporter for real-time imaging of gene expression in cell cultures and in live animals
         description: Light-emitting reporter proteins play an increasing role in the study of gene expression in vitro and in vivo. Here we present a ruc-gfp fusion gene construct generated by fusing a cDNA for Renilla luciferase (ruc) in-frame with a cDNA encoding the 'humanized' GFP (gfp) from Aequorea. A plasmid containing the fusion gene construct was successfully transformed into, and expressed in, mammalian cells. The transformed cells exhibited both Renilla luciferase activity in the presence of coelenterazine and GFP fluorescence upon excitation with UV light. Spectrofluorometry of cells containing the Ruc-GFP fusion protein, in the absence of wavelengths capable of exciting GFP fluorescence but in the presence of the luciferase substrate, coelenterazine, showed an emission spectrum with two peaks at 475 nm and 508 nm. These two peaks correspond to the emission maximum of Renilla luciferase at 475 nm and that of GFP at 508 nm. The peak at 508 nm generated in the presence of coelenterazine alone (without UV excitation) is the result of intramolecular energy transfer from Renilla luciferase to Aequorea GFP. Southern analysis of genomic DNA purified from transformed Chinese hamster ovary (CHO) cells and fluorescence in situ hybridization (FISH) to metaphase chromosomes confirmed the integration of the ruc-gfp fusion gene on a single chromosome. The bifunctional Ruc-GFP fusion protein allows the detection of gene expression at the single-cell level based on green fluorescence, and in a group of cells based on luminescence emission. Furthermore, animal experiments revealed that light emission from the Ruc-GFP fusion protein can be detected externally in the organs or tissues of live animals bearing the gene construct.
         datePublished:
         dateModified:
         pageStart:160
         pageEnd:168
         sameAs:https://doi.org/10.1007/s00438-002-0751-9
         keywords:
            Renilla luciferase Aequorea GFP (Ruc-GFP) fusion protein Reporter gene Renilla luciferase Aequorea GFP Fluorescence in situ hybridization (FISH)
            Plant Genetics and Genomics
            Human Genetics
            Microbial Genetics and Genomics
            Animal Genetics and Genomics
            Biochemistry
            general
         image:
         isPartOf:
            name:Molecular Genetics and Genomics
            issn:
               1617-4623
               1617-4615
            volumeNumber:268
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name: Y. Wang
               affiliation:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                     address:
                        name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: Y. Yu
               affiliation:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                     address:
                        name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: S. Shabahang
               affiliation:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                     address:
                        name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: G. Wang
               affiliation:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                     address:
                        name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: A. Szalay
               affiliation:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                     address:
                        name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Renilla luciferase-Aequorea GFP (Ruc-GFP) fusion protein, a novel dual reporter for real-time imaging of gene expression in cell cultures and in live animals
      description: Light-emitting reporter proteins play an increasing role in the study of gene expression in vitro and in vivo. Here we present a ruc-gfp fusion gene construct generated by fusing a cDNA for Renilla luciferase (ruc) in-frame with a cDNA encoding the 'humanized' GFP (gfp) from Aequorea. A plasmid containing the fusion gene construct was successfully transformed into, and expressed in, mammalian cells. The transformed cells exhibited both Renilla luciferase activity in the presence of coelenterazine and GFP fluorescence upon excitation with UV light. Spectrofluorometry of cells containing the Ruc-GFP fusion protein, in the absence of wavelengths capable of exciting GFP fluorescence but in the presence of the luciferase substrate, coelenterazine, showed an emission spectrum with two peaks at 475 nm and 508 nm. These two peaks correspond to the emission maximum of Renilla luciferase at 475 nm and that of GFP at 508 nm. The peak at 508 nm generated in the presence of coelenterazine alone (without UV excitation) is the result of intramolecular energy transfer from Renilla luciferase to Aequorea GFP. Southern analysis of genomic DNA purified from transformed Chinese hamster ovary (CHO) cells and fluorescence in situ hybridization (FISH) to metaphase chromosomes confirmed the integration of the ruc-gfp fusion gene on a single chromosome. The bifunctional Ruc-GFP fusion protein allows the detection of gene expression at the single-cell level based on green fluorescence, and in a group of cells based on luminescence emission. Furthermore, animal experiments revealed that light emission from the Ruc-GFP fusion protein can be detected externally in the organs or tissues of live animals bearing the gene construct.
      datePublished:
      dateModified:
      pageStart:160
      pageEnd:168
      sameAs:https://doi.org/10.1007/s00438-002-0751-9
      keywords:
         Renilla luciferase Aequorea GFP (Ruc-GFP) fusion protein Reporter gene Renilla luciferase Aequorea GFP Fluorescence in situ hybridization (FISH)
         Plant Genetics and Genomics
         Human Genetics
         Microbial Genetics and Genomics
         Animal Genetics and Genomics
         Biochemistry
         general
      image:
      isPartOf:
         name:Molecular Genetics and Genomics
         issn:
            1617-4623
            1617-4615
         volumeNumber:268
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name: Y. Wang
            affiliation:
                  name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                  address:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: Y. Yu
            affiliation:
                  name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                  address:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: S. Shabahang
            affiliation:
                  name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                  address:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: G. Wang
            affiliation:
                  name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                  address:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: A. Szalay
            affiliation:
                  name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
                  address:
                     name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Molecular Genetics and Genomics
      issn:
         1617-4623
         1617-4615
      volumeNumber:268
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
      address:
         name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
         type:PostalAddress
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
      address:
         name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
         type:PostalAddress
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
      address:
         name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
         type:PostalAddress
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
      address:
         name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
         type:PostalAddress
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
      address:
         name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name: Y. Wang
      affiliation:
            name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
            address:
               name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
               type:PostalAddress
            type:Organization
      name: Y. Yu
      affiliation:
            name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
            address:
               name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
               type:PostalAddress
            type:Organization
      name: S. Shabahang
      affiliation:
            name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
            address:
               name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
               type:PostalAddress
            type:Organization
      name: G. Wang
      affiliation:
            name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
            address:
               name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
               type:PostalAddress
            type:Organization
      name: A. Szalay
      affiliation:
            name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA
            address:
               name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
      name:Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350, USA,
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(33)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.54s.

LINK . SPRINGER . COM {}