We are analyzing https://link.springer.com/article/10.1007/s00438-002-0711-4.

Title:
Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.) | Molecular Genetics and Genomics
Description:
A linear plasmid molecule about 11 kb in length is present in the mitochondria of some varieties of rapeseed (Brassica napus L.). This plasmid can be inherited from the male parent, through the pollen, as well as by the usual maternal route, although the main mitochondrial genome is maternally inherited in rapeseed. We determined the complete nucleotide sequence of this plasmid DNA and clarified its genetic organization. The length of the linear plasmid is 11,640 bp. At the termini of the plasmid molecule are inverted repeats of 327 bp. The GC content of the plasmid DNA is 30.9%; thus, the plasmid is quite AT-rich compared to the main mitochondrial genome in higher plants. The plasmid has six ORFs, two of which encode a phage-type DNA polymerase and a phage-type RNA polymerase, respectively. RT-PCR analyses revealed that all six ORFs are transcribed, and all four ORFs on the minus strand are probably cotranscribed from a single promoter located in the terminal inverted repeat. We also show here that at least three of the six ORFs are translated into proteins in rapeseed mitochondria, and expressed at relatively high levels in flowers, as shown by Western analysis. These results suggest that this linear DNA molecule is able to replicate as an autonomous replicon and to express the genes it carries in rapeseed mitochondria.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

article, plasmid, content, analysis, linear, mitochondrial, rapeseed, brassica, napus, access, privacy, cookies, mitochondria, information, publish, research, search, genome, dna, orfs, data, main, log, journal, genomics, expression, handa, itani, sato, molecule, related, open, discover, springer, optional, personal, parties, policy, find, track, molecular, genetics, structural, features, cite, explore, length, inherited, male, pollen,

Topics {✒️}

month download article/chapter phage-type rna polymerase rt-pcr analyses revealed phage-type dna polymerase main mitochondrial genome article molecular genetics privacy choices/manage cookies related subjects linear mitochondrial plasmid full article pdf key genes related comparative transcriptome analysis european economic area original paper published scope submit manuscript usual maternal route complete nucleotide sequence single promoter located plasmodiophora brassicae nuclear hitsuji-ga-oka linear dna molecule conditions privacy policy terminal inverted repeat linear plasmid molecule accepting optional cookies check access instant access brassica napus main content log journal finder publish male parent article log structural features article cite privacy policy linear plasmid personal data expression analysis article handa books a rapeseed mitochondria plasmid dna optional cookies plasmid molecule manage preferences genomics aims usage analysis data protection western analysis essential cookies

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.)
         description: A linear plasmid molecule about 11 kb in length is present in the mitochondria of some varieties of rapeseed (Brassica napus L.). This plasmid can be inherited from the male parent, through the pollen, as well as by the usual maternal route, although the main mitochondrial genome is maternally inherited in rapeseed. We determined the complete nucleotide sequence of this plasmid DNA and clarified its genetic organization. The length of the linear plasmid is 11,640 bp. At the termini of the plasmid molecule are inverted repeats of 327 bp. The GC content of the plasmid DNA is 30.9%; thus, the plasmid is quite AT-rich compared to the main mitochondrial genome in higher plants. The plasmid has six ORFs, two of which encode a phage-type DNA polymerase and a phage-type RNA polymerase, respectively. RT-PCR analyses revealed that all six ORFs are transcribed, and all four ORFs on the minus strand are probably cotranscribed from a single promoter located in the terminal inverted repeat. We also show here that at least three of the six ORFs are translated into proteins in rapeseed mitochondria, and expressed at relatively high levels in flowers, as shown by Western analysis. These results suggest that this linear DNA molecule is able to replicate as an autonomous replicon and to express the genes it carries in rapeseed mitochondria.
         datePublished:
         dateModified:
         pageStart:797
         pageEnd:805
         sameAs:https://doi.org/10.1007/s00438-002-0711-4
         keywords:
            Linear plasmid Mitochondria Brassica napus L. Paternal transmission Gene expression
            Plant Genetics and Genomics
            Human Genetics
            Microbial Genetics and Genomics
            Animal Genetics and Genomics
            Biochemistry
            general
         image:
         isPartOf:
            name:Molecular Genetics and Genomics
            issn:
               1617-4623
               1617-4615
            volumeNumber:267
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name: H. Handa
               affiliation:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                     address:
                        name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: K. Itani
               affiliation:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                     address:
                        name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                        type:PostalAddress
                     type:Organization
               type:Person
               name: H. Sato
               affiliation:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                     address:
                        name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.)
      description: A linear plasmid molecule about 11 kb in length is present in the mitochondria of some varieties of rapeseed (Brassica napus L.). This plasmid can be inherited from the male parent, through the pollen, as well as by the usual maternal route, although the main mitochondrial genome is maternally inherited in rapeseed. We determined the complete nucleotide sequence of this plasmid DNA and clarified its genetic organization. The length of the linear plasmid is 11,640 bp. At the termini of the plasmid molecule are inverted repeats of 327 bp. The GC content of the plasmid DNA is 30.9%; thus, the plasmid is quite AT-rich compared to the main mitochondrial genome in higher plants. The plasmid has six ORFs, two of which encode a phage-type DNA polymerase and a phage-type RNA polymerase, respectively. RT-PCR analyses revealed that all six ORFs are transcribed, and all four ORFs on the minus strand are probably cotranscribed from a single promoter located in the terminal inverted repeat. We also show here that at least three of the six ORFs are translated into proteins in rapeseed mitochondria, and expressed at relatively high levels in flowers, as shown by Western analysis. These results suggest that this linear DNA molecule is able to replicate as an autonomous replicon and to express the genes it carries in rapeseed mitochondria.
      datePublished:
      dateModified:
      pageStart:797
      pageEnd:805
      sameAs:https://doi.org/10.1007/s00438-002-0711-4
      keywords:
         Linear plasmid Mitochondria Brassica napus L. Paternal transmission Gene expression
         Plant Genetics and Genomics
         Human Genetics
         Microbial Genetics and Genomics
         Animal Genetics and Genomics
         Biochemistry
         general
      image:
      isPartOf:
         name:Molecular Genetics and Genomics
         issn:
            1617-4623
            1617-4615
         volumeNumber:267
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name: H. Handa
            affiliation:
                  name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                  address:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: K. Itani
            affiliation:
                  name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                  address:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                     type:PostalAddress
                  type:Organization
            type:Person
            name: H. Sato
            affiliation:
                  name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
                  address:
                     name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Molecular Genetics and Genomics
      issn:
         1617-4623
         1617-4615
      volumeNumber:267
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
      address:
         name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
         type:PostalAddress
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
      address:
         name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
         type:PostalAddress
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
      address:
         name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name: H. Handa
      affiliation:
            name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
            address:
               name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
               type:PostalAddress
            type:Organization
      name: K. Itani
      affiliation:
            name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
            address:
               name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
               type:PostalAddress
            type:Organization
      name: H. Sato
      affiliation:
            name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan
            address:
               name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
      name:Laboratory of Plant Genecology, National Agricultural Research Center for Hokkaido Region, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan,
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(29)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.89s.

LINK . SPRINGER . COM {}