We are analyzing https://link.springer.com/article/10.1007/s00436-014-4166-4.

Title:
Suitable in vitro Eimeria arloingi macromeront formation in host endothelial cells and modulation of adhesion molecule, cytokine and chemokine gene transcription | Parasitology Research
Description:
Eimeria arloingi infections can cause severe haemorrhagic enteritis in young goat kids, thereby leading to high economic losses in goat industry worldwide. We aimed to isolate a new E. arloingi strain and establish a suitable in vitro culture system for the first merogony. E. arloingi oocysts were collected from naturally infected goat kids in the province of Alentejo, Portugal. For the maintenance of E. arloingi (strain A), kids kept under strict parasite-free conditions were orally infected with 103 sporulated oocysts each. Further, a new excystation protocol was successfully established to obtain viable sporozoites for further in vitro development in primary bovine umbilical vein endothelial cells (BUVEC). Overall, E. arloingi first merogony was successfully accomplished in BUVEC leading to macromeront formation (up to 150 μm) and the release of fully developed merozoites I stages. Moreover, host endothelial cell-parasite interactions were investigated in order to determine the extent of modulation carried out by E. arloingi in BUVEC during the first merogony. Gene transcription of adhesion molecules (E-selectin, P-selectin, VCAM-1, ICAM-1) was enhanced in the first hours post-infection (p.i.) in E. arloingi-infected BUVEC. BUVEC activation due to invasion was also shown by increased chemokine (CXCL8, CCL2, CCL5), cytokine (GM-CSF) and COX-2 gene transcription. The new E. arloingi (strain A) will be useful for better comprehension of early host innate immune reactions against this parasite in vitro/in vivo as well as to further our investigations in the complex Eimeria-host endothelial cell interactions.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Video & Online Content

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 8,280,528 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

article, google, scholar, pubmed, eimeria, cas, endothelial, cells, hermosilla, arloingi, taubert, res, parasitol, vet, vitro, host, bovine, parasitology, silva, cell, bovis, access, zahner, plasmodium, research, transcription, cortes, kids, development, parasite, dois, coccidiosis, privacy, cookies, content, adhesion, gene, infections, goat, infected, buvec, goats, falciparum, publish, search, cytokine, liliana, vilaviçosa, strain, open,

Topics {✒️}

related subjects parasite-host cell interactions month download article/chapter strict parasite-free conditions anja taubert & carlos hermosilla host endothelial cells eimeria maxima-induced transcriptional madin-darby bovine kidney 4-dihydr o-2h-benzo[ microarray-based transcriptional profiling life cycle iifa/universidade de évora bovine endothelial cells endothelial cells infected permanent cell lines antigen-induced cytokine production full article pdf check access instant access eimeria bovis primary experimental caprine coccidiosis reduces parasite growth chemokine gene transcription privacy choices/manage cookies cultured bovine cells cox-2 gene transcription adaptive immune response host cells springer mi-ichi bovine fetal gastrointestinal núcleo da mitra article silva challenge infected calves vitro development helminth parasitic infections west ukraine region goat industry worldwide conditions privacy policy arloingi-infected buvec european economic area severe haemorrhagic enteritis high economic losses fully developed merozoites calcium ionophore a23187 jacobs wr jr stearoyl-coa desaturase potential therapeutic target semi-arid zones excellent technical assistance strategic project pest

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Suitable in vitro Eimeria arloingi macromeront formation in host endothelial cells and modulation of adhesion molecule, cytokine and chemokine gene transcription
         description: Eimeria arloingi infections can cause severe haemorrhagic enteritis in young goat kids, thereby leading to high economic losses in goat industry worldwide. We aimed to isolate a new E. arloingi strain and establish a suitable in vitro culture system for the first merogony. E. arloingi oocysts were collected from naturally infected goat kids in the province of Alentejo, Portugal. For the maintenance of E. arloingi (strain A), kids kept under strict parasite-free conditions were orally infected with 103 sporulated oocysts each. Further, a new excystation protocol was successfully established to obtain viable sporozoites for further in vitro development in primary bovine umbilical vein endothelial cells (BUVEC). Overall, E. arloingi first merogony was successfully accomplished in BUVEC leading to macromeront formation (up to 150 μm) and the release of fully developed merozoites I stages. Moreover, host endothelial cell-parasite interactions were investigated in order to determine the extent of modulation carried out by E. arloingi in BUVEC during the first merogony. Gene transcription of adhesion molecules (E-selectin, P-selectin, VCAM-1, ICAM-1) was enhanced in the first hours post-infection (p.i.) in E. arloingi-infected BUVEC. BUVEC activation due to invasion was also shown by increased chemokine (CXCL8, CCL2, CCL5), cytokine (GM-CSF) and COX-2 gene transcription. The new E. arloingi (strain A) will be useful for better comprehension of early host innate immune reactions against this parasite in vitro/in vivo as well as to further our investigations in the complex Eimeria-host endothelial cell interactions.
         datePublished:2014-10-23T00:00:00Z
         dateModified:2014-10-23T00:00:00Z
         pageStart:113
         pageEnd:124
         sameAs:https://doi.org/10.1007/s00436-014-4166-4
         keywords:
             Eimeria arloingi
            Coccidiosis
            Endothelial host cells
            In vitro
            Pro-inflammatory molecules
            Medical Microbiology
            Microbiology
            Immunology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig6_HTML.gif
         isPartOf:
            name:Parasitology Research
            issn:
               1432-1955
               0932-0113
            volumeNumber:114
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Berlin Heidelberg
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Liliana M. R. Silva
               affiliation:
                     name:IIFA/Universidade de Évora, Núcleo da Mitra
                     address:
                        name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                        type:PostalAddress
                     type:Organization
                     name:Justus Liebig University Giessen
                     address:
                        name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Maria J. M. Vila-Viçosa
               affiliation:
                     name:IIFA/Universidade de Évora, Núcleo da Mitra
                     address:
                        name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Helder C. E. Cortes
               affiliation:
                     name:IIFA/Universidade de Évora, Núcleo da Mitra
                     address:
                        name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Anja Taubert
               affiliation:
                     name:Justus Liebig University Giessen
                     address:
                        name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Carlos Hermosilla
               affiliation:
                     name:Justus Liebig University Giessen
                     address:
                        name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Suitable in vitro Eimeria arloingi macromeront formation in host endothelial cells and modulation of adhesion molecule, cytokine and chemokine gene transcription
      description: Eimeria arloingi infections can cause severe haemorrhagic enteritis in young goat kids, thereby leading to high economic losses in goat industry worldwide. We aimed to isolate a new E. arloingi strain and establish a suitable in vitro culture system for the first merogony. E. arloingi oocysts were collected from naturally infected goat kids in the province of Alentejo, Portugal. For the maintenance of E. arloingi (strain A), kids kept under strict parasite-free conditions were orally infected with 103 sporulated oocysts each. Further, a new excystation protocol was successfully established to obtain viable sporozoites for further in vitro development in primary bovine umbilical vein endothelial cells (BUVEC). Overall, E. arloingi first merogony was successfully accomplished in BUVEC leading to macromeront formation (up to 150 μm) and the release of fully developed merozoites I stages. Moreover, host endothelial cell-parasite interactions were investigated in order to determine the extent of modulation carried out by E. arloingi in BUVEC during the first merogony. Gene transcription of adhesion molecules (E-selectin, P-selectin, VCAM-1, ICAM-1) was enhanced in the first hours post-infection (p.i.) in E. arloingi-infected BUVEC. BUVEC activation due to invasion was also shown by increased chemokine (CXCL8, CCL2, CCL5), cytokine (GM-CSF) and COX-2 gene transcription. The new E. arloingi (strain A) will be useful for better comprehension of early host innate immune reactions against this parasite in vitro/in vivo as well as to further our investigations in the complex Eimeria-host endothelial cell interactions.
      datePublished:2014-10-23T00:00:00Z
      dateModified:2014-10-23T00:00:00Z
      pageStart:113
      pageEnd:124
      sameAs:https://doi.org/10.1007/s00436-014-4166-4
      keywords:
          Eimeria arloingi
         Coccidiosis
         Endothelial host cells
         In vitro
         Pro-inflammatory molecules
         Medical Microbiology
         Microbiology
         Immunology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00436-014-4166-4/MediaObjects/436_2014_4166_Fig6_HTML.gif
      isPartOf:
         name:Parasitology Research
         issn:
            1432-1955
            0932-0113
         volumeNumber:114
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Berlin Heidelberg
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Liliana M. R. Silva
            affiliation:
                  name:IIFA/Universidade de Évora, Núcleo da Mitra
                  address:
                     name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                     type:PostalAddress
                  type:Organization
                  name:Justus Liebig University Giessen
                  address:
                     name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Maria J. M. Vila-Viçosa
            affiliation:
                  name:IIFA/Universidade de Évora, Núcleo da Mitra
                  address:
                     name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Helder C. E. Cortes
            affiliation:
                  name:IIFA/Universidade de Évora, Núcleo da Mitra
                  address:
                     name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Anja Taubert
            affiliation:
                  name:Justus Liebig University Giessen
                  address:
                     name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Carlos Hermosilla
            affiliation:
                  name:Justus Liebig University Giessen
                  address:
                     name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Parasitology Research
      issn:
         1432-1955
         0932-0113
      volumeNumber:114
Organization:
      name:Springer Berlin Heidelberg
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:IIFA/Universidade de Évora, Núcleo da Mitra
      address:
         name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
         type:PostalAddress
      name:Justus Liebig University Giessen
      address:
         name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
         type:PostalAddress
      name:IIFA/Universidade de Évora, Núcleo da Mitra
      address:
         name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
         type:PostalAddress
      name:IIFA/Universidade de Évora, Núcleo da Mitra
      address:
         name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
         type:PostalAddress
      name:Justus Liebig University Giessen
      address:
         name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
         type:PostalAddress
      name:Justus Liebig University Giessen
      address:
         name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Liliana M. R. Silva
      affiliation:
            name:IIFA/Universidade de Évora, Núcleo da Mitra
            address:
               name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
               type:PostalAddress
            type:Organization
            name:Justus Liebig University Giessen
            address:
               name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Maria J. M. Vila-Viçosa
      affiliation:
            name:IIFA/Universidade de Évora, Núcleo da Mitra
            address:
               name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
               type:PostalAddress
            type:Organization
      name:Helder C. E. Cortes
      affiliation:
            name:IIFA/Universidade de Évora, Núcleo da Mitra
            address:
               name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
               type:PostalAddress
            type:Organization
      name:Anja Taubert
      affiliation:
            name:Justus Liebig University Giessen
            address:
               name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
               type:PostalAddress
            type:Organization
      name:Carlos Hermosilla
      affiliation:
            name:Justus Liebig University Giessen
            address:
               name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
      name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
      name:ICAAM – Instituto Ciências Agrárias e Ambientais Mediterrânicas, IIFA/Universidade de Évora, Núcleo da Mitra, Évora, Portugal
      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(145)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js
Video.js

CDN Services {📦}

Crossref
Net/play">

LINK . SPRINGER . COM {}