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We are analyzing https://link.springer.com/article/10.1007/s00432-010-0777-z.

Title:
Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis | Journal of Cancer Research and Clinical Oncology
Description:
Purpose GADD45 is a family of proteins involved in DNA damage response and cell growth arrest. GADD45G was identified as an interleukin-2-induced immediate-early gene, and methylation of GADD45G was studied in various tumor cell lines and a few primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. Methods In our study, we used HRM analysis to detect the methylation levels of GADD45G gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues. Results The frequency of GADD45G methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of GADD45G were inversely correlated with methylation levels. But we did not find significant association between GADD45G methylation status and TNM staging in all three types of cancers. Conclusions In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of GADD45G. And this is the first study to evaluate the prevalence of GADD45G methylation based on large amount of tumor samples, showing that epigenetic regulation of GADD45G was associated with carcinogenesis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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Keywords {πŸ”}

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Topics {βœ’οΈ}

methylation-sensitive high-resolution melting stress-responsive mtk1/mekk4 mapkkk shenzhen-pku-hkust medical center month download article/chapter age-related dna methylation real-time quantitative pcr cdc2/cyclinb1 kinase inhibitors high-resolution melting stress-responsive gene gadd45g gadd45-gamma methylation levels dna damage response quantitative methylation analysis high-resolution genotyping amplicon melting analysis full article pdf growth inhibitory gene semi-quantitative detection stress-inducible gadd45 cell growth arrest dna methylation signature dna methylation markers growth arrest signals fornace aj jr anaplastic thyroid cancer privacy choices/manage cookies clinical oncology aims tumor suppressive roles methylation-specific pcr related subjects primary acute leukemia dna-damaging agents individual dna strands bisulphite-treated dna cell cycle checkpoints gadd45g methylation based cancer research cdna encoding myd118 negative growth control wan contributed equally high sensitivity mapping decitabine-induced demethylation bo yu cpg island methylation primary tumor samples promoter methylation cpg methylation density tumor cell lines article zhang human pituitary adenomas human hepatocellular carcinoma

Schema {πŸ—ΊοΈ}

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         description:GADD45 is a family of proteins involved in DNA damage response and cell growth arrest. GADD45G was identified as an interleukin-2-induced immediate-early gene, and methylation of GADD45G was studied in various tumor cell lines and a few primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. In our study, we used HRM analysis to detect the methylation levels of GADD45G gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues. The frequency of GADD45G methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of GADD45G were inversely correlated with methylation levels. But we did not find significant association between GADD45G methylation status and TNM staging in all three types of cancers. In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of GADD45G. And this is the first study to evaluate the prevalence of GADD45G methylation based on large amount of tumor samples, showing that epigenetic regulation of GADD45G was associated with carcinogenesis.
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      headline:Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis
      description:GADD45 is a family of proteins involved in DNA damage response and cell growth arrest. GADD45G was identified as an interleukin-2-induced immediate-early gene, and methylation of GADD45G was studied in various tumor cell lines and a few primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. In our study, we used HRM analysis to detect the methylation levels of GADD45G gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues. The frequency of GADD45G methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of GADD45G were inversely correlated with methylation levels. But we did not find significant association between GADD45G methylation status and TNM staging in all three types of cancers. In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of GADD45G. And this is the first study to evaluate the prevalence of GADD45G methylation based on large amount of tumor samples, showing that epigenetic regulation of GADD45G was associated with carcinogenesis.
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