We are analyzing https://link.springer.com/article/10.1007/s00432-004-0619-y.

Title:
Increased expression of ADAM family members in human breast cancer and breast cancer cell lines | Journal of Cancer Research and Clinical Oncology
Description:
Purpose ADAMs (A Disintegrin and Metalloprotease) are multifunctional, membrane-bound cell surface glycoproteins, which have numerous functions in cell growth, differentiation, and motility. We wished to investigate the expression of ADAM 9, 10, 12, 15, and in human breast cancer. Methods Expression of ADAMs was determined in breast cancer specimens and the corresponding non-neoplastic breast tissue from 24 patients, and in the MCF-7 and MDA-MB 453 breast cancer cell lines via quantitative RT-PCR and immunohistochemistry. The effects of anti-ADAM antibodies on cell proliferation were assessed by measuring DNA-synthesis. Results Breast cancer tissue samples showed increased mRNA expression of ADAM 9, 12, and 17, whereas ADAM 10 and 15 were not differently expressed. Protein expression was studied by immunohistochemistry. All ADAMs were expressed in MCF-7 and MDA-MB453 cell lines, with the highest expression levels being observed for ADAM 9, 12, and 17. Application of anti-ADAM 15 and anti-ADAM 17 antibodies significantly inhibited the proliferation of both MCF-7 and MDA-MB453 breast cancer cell lines. In contrast, the growth of MCF-7 cells appeared to be stimulated by the administration of anti-ADAM 12 antibody. Conclusion The results of this study suggest that ADAMs are differentially expressed in human breast cancer and are capable of modulating tumour cell growth.
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releases tumour-necrosis factor-alpha cell-surface proteins shed month download article/chapter mda-mb453 cell lines triple-negative breast cancer g-protein-coupled receptor quantitative rt-pcr otto-von-guericke-university promoting cell-cell interactions stacy carl-mcgrath c-erbb-2/her2/neu regulated alpha-secretase cleavage induce tgf-beta1 production acidic integrin-binding motifs neoplastic breast tissue tgf-beta-regulated expression human breast cancer breast cancer specimens protease domain full article pdf breast cancer progression usage analysis modular proteins capable privacy choices/manage cookies author information authors clinical oncology aims related subjects human immune cells app alpha-secretase human pancreatic cancer highest expression levels messenger rna expression anti-adam antibodies mediates cellular adhesion membrane proteins cell-matrix interactions integrin-mediated control breast cancer protein-ectodomain shedding increased expression inflammatory cytokine production multiple beta1 integrins protein expression human liver cancers anti-adam 12 antibody european economic area measuring dna-synthesis mcf-7 cells appeared regulate dna synthesis triggers signaling events

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         description: ADAMs (A Disintegrin and Metalloprotease) are multifunctional, membrane-bound cell surface glycoproteins, which have numerous functions in cell growth, differentiation, and motility. We wished to investigate the expression of ADAM 9, 10, 12, 15, and in human breast cancer. Expression of ADAMs was determined in breast cancer specimens and the corresponding non-neoplastic breast tissue from 24 patients, and in the MCF-7 and MDA-MB 453 breast cancer cell lines via quantitative RT-PCR and immunohistochemistry. The effects of anti-ADAM antibodies on cell proliferation were assessed by measuring DNA-synthesis. Breast cancer tissue samples showed increased mRNA expression of ADAM 9, 12, and 17, whereas ADAM 10 and 15 were not differently expressed. Protein expression was studied by immunohistochemistry. All ADAMs were expressed in MCF-7 and MDA-MB453 cell lines, with the highest expression levels being observed for ADAM 9, 12, and 17. Application of anti-ADAM 15 and anti-ADAM 17 antibodies significantly inhibited the proliferation of both MCF-7 and MDA-MB453 breast cancer cell lines. In contrast, the growth of MCF-7 cells appeared to be stimulated by the administration of anti-ADAM 12 antibody. The results of this study suggest that ADAMs are differentially expressed in human breast cancer and are capable of modulating tumour cell growth.
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