We are analyzing https://link.springer.com/article/10.1007/s00425-008-0885-z.

Title:
PtFLC homolog from trifoliate orange (Poncirus trifoliata) is regulated by alternative splicing and experiences seasonal fluctuation in expression level | Planta
Description:
In many plant species, exposure to a prolonged period of low temperature during the winter promotes flowering in the spring, a process termed vernalization. In Arabidopsis, the vernalization requirement of winter annual ecotypes is caused by a MADS-box gene FLOWERING LOCUS C (FLC), which is a repressor of flowering gene. Here, a MADS-box gene was isolated from an early flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata L. Raf) by the RACE method combined with a cDNA library. Phylogenetic analysis reveals that the MADS-box gene is more closely related to the homologs of the FLOWERING LOCUS C lineage than to any of the other MIKC-type MADS-box lineages known from Arabidopsis. The expression profile of the MADS-box gene by real-time PCR showed upregulation of PtFLC during the winter, followed by a decrease in the spring and summer. This kind of cycling is contrary to the pattern observed in Arabidopsis. In situ hybridization reveals that the MADS-box gene is predominately expressed in the vegetative and reproductive meristems. In addition, five alternatively spliced transcripts of the MADS-box gene were also isolated at juvenile and adult mutant developmental stages. Expression analysis of these transcripts at different developmental stages indicated involvement of alternative splicing during phase change. The information suggests a complicated regulation mechanism in seasonal response and flower formation in perennial woody plants.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

article, google, scholar, cas, pubmed, flowering, plant, madsbox, arabidopsis, gene, splicing, vernalization, plants, genes, mol, sci, alternative, expression, locus, flc, dormancy, bot, analysis, trifoliate, orange, temperature, access, citrus, usa, biol, privacy, cookies, content, information, seasonal, zhang, cell, time, science, publish, research, search, planta, poncirus, trifoliata, winter, repressor, phylogenetic, related, hybridization,

Topics {✒️}

org/pb2006/public/p03/p03015 mikc-type mads-box lineages month download article/chapter chun-gen hu conserved c-terminal motifs mads-domain transcription factor typeii mads-box genes wild-type arabidopsis revealed genome-wide expression analyses nuclear pre-mrna splicing mads-box family horticultural plant biology full article pdf article planta aims precocious trifoliate orange mads domain protein privacy choices/manage cookies mads-box gene situ hybridization reveals agamous pre-mrna mads-box genes simpson gg pre-mrna splicing process termed vernalization mads-box transcripts specific plant genes arabidopsis proteins apetala3 zhi-min li flowering-time regulators plant biologists meeting intra-reproductive vernalization related subjects european economic area race method combined experienced faster birth increased cift abundance stem correlates race methods combined huazhong agricultural university rna binding proteins citrus bud cultures factors affecting flowering li contributed equally conditions privacy policy comparative phylogenetic analysis ptflc homolog arabidopsis thaliana flowering experiences seasonal fluctuation article zhang alternatively spliced transcripts

Questions {❓}

Simpson GG, Dean C (2002) Arabidopsis, the Rosetta stone of flowering time?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:PtFLC homolog from trifoliate orange (Poncirus trifoliata) is regulated by alternative splicing and experiences seasonal fluctuation in expression level
         description:In many plant species, exposure to a prolonged period of low temperature during the winter promotes flowering in the spring, a process termed vernalization. In Arabidopsis, the vernalization requirement of winter annual ecotypes is caused by a MADS-box gene FLOWERING LOCUS C (FLC), which is a repressor of flowering gene. Here, a MADS-box gene was isolated from an early flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata L. Raf) by the RACE method combined with a cDNA library. Phylogenetic analysis reveals that the MADS-box gene is more closely related to the homologs of the FLOWERING LOCUS C lineage than to any of the other MIKC-type MADS-box lineages known from Arabidopsis. The expression profile of the MADS-box gene by real-time PCR showed upregulation of PtFLC during the winter, followed by a decrease in the spring and summer. This kind of cycling is contrary to the pattern observed in Arabidopsis. In situ hybridization reveals that the MADS-box gene is predominately expressed in the vegetative and reproductive meristems. In addition, five alternatively spliced transcripts of the MADS-box gene were also isolated at juvenile and adult mutant developmental stages. Expression analysis of these transcripts at different developmental stages indicated involvement of alternative splicing during phase change. The information suggests a complicated regulation mechanism in seasonal response and flower formation in perennial woody plants.
         datePublished:2009-01-06T00:00:00Z
         dateModified:2009-01-06T00:00:00Z
         pageStart:847
         pageEnd:859
         sameAs:https://doi.org/10.1007/s00425-008-0885-z
         keywords:
            Alternative splicing
             FLOWERING LOCUS C (FLC)
            Flowering time
            In situ hybridization
            Precocious trifoliate orange
            Vernalization
            Plant Sciences
            Agriculture
            Ecology
            Forestry
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig6_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig7_HTML.gif
         isPartOf:
            name:Planta
            issn:
               1432-2048
               0032-0935
            volumeNumber:229
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Jin-Zhi Zhang
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Zhi-Min Li
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Li Mei
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jia-Ling Yao
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chun-Gen Hu
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:PtFLC homolog from trifoliate orange (Poncirus trifoliata) is regulated by alternative splicing and experiences seasonal fluctuation in expression level
      description:In many plant species, exposure to a prolonged period of low temperature during the winter promotes flowering in the spring, a process termed vernalization. In Arabidopsis, the vernalization requirement of winter annual ecotypes is caused by a MADS-box gene FLOWERING LOCUS C (FLC), which is a repressor of flowering gene. Here, a MADS-box gene was isolated from an early flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata L. Raf) by the RACE method combined with a cDNA library. Phylogenetic analysis reveals that the MADS-box gene is more closely related to the homologs of the FLOWERING LOCUS C lineage than to any of the other MIKC-type MADS-box lineages known from Arabidopsis. The expression profile of the MADS-box gene by real-time PCR showed upregulation of PtFLC during the winter, followed by a decrease in the spring and summer. This kind of cycling is contrary to the pattern observed in Arabidopsis. In situ hybridization reveals that the MADS-box gene is predominately expressed in the vegetative and reproductive meristems. In addition, five alternatively spliced transcripts of the MADS-box gene were also isolated at juvenile and adult mutant developmental stages. Expression analysis of these transcripts at different developmental stages indicated involvement of alternative splicing during phase change. The information suggests a complicated regulation mechanism in seasonal response and flower formation in perennial woody plants.
      datePublished:2009-01-06T00:00:00Z
      dateModified:2009-01-06T00:00:00Z
      pageStart:847
      pageEnd:859
      sameAs:https://doi.org/10.1007/s00425-008-0885-z
      keywords:
         Alternative splicing
          FLOWERING LOCUS C (FLC)
         Flowering time
         In situ hybridization
         Precocious trifoliate orange
         Vernalization
         Plant Sciences
         Agriculture
         Ecology
         Forestry
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig6_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00425-008-0885-z/MediaObjects/425_2008_885_Fig7_HTML.gif
      isPartOf:
         name:Planta
         issn:
            1432-2048
            0032-0935
         volumeNumber:229
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jin-Zhi Zhang
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Zhi-Min Li
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Li Mei
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jia-Ling Yao
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chun-Gen Hu
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Planta
      issn:
         1432-2048
         0032-0935
      volumeNumber:229
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Huazhong Agricultural University
      address:
         name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jin-Zhi Zhang
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Zhi-Min Li
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Li Mei
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Jia-Ling Yao
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Chun-Gen Hu
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
      name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
      name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
      name:College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
      name:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(148)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.48s.

LINK . SPRINGER . COM {}