We are analyzing https://link.springer.com/article/10.1007/s00424-007-0377-1.

Title:
Kv1 and Kir2 potassium channels are expressed in rat brain endothelial cells | Pflügers Archiv - European Journal of Physiology
Description:
The endothelial cells of the brain microvasculature, which constitute the blood–brain barrier, secrete K+ into brain interstitial fluid. K+ channels are predicted to have a central role to play in this process. The aim of the following study was to characterise K+ channels in primary cultures of endothelial cells isolated from rat brain microvessels by whole-cell patch clamp and real-time polymerase chain reaction. In the 4 h after plating, the rat brain endothelial cells expressed predominantly a depolarisation-activated delayed-rectifying outward K+ conductance and a time-independent inwardly rectifying K+ conductance prominent at hyperpolarising potentials. The outward current was inhibited by 1 mM 4-aminopyridine (4AP), 10 nM margatoxin and 100 nM dendrotoxin-K, indicating the involvement of Kv1 channels. The half maximal activation voltage and time constants of activation and inactivation of the 4AP-sensitive current were similar to Kv1.3. The inwardly rectifying conductance was inhibited by Ba2+ in a dose- and voltage-dependent fashion; the kinetics of which resembled Kir2 channels. Quantification of messenger ribonucleic acid transcripts revealed Kv1.3 > 1.2 = 1.4 = 1.5 = 1.6 and Kir2.1 = 2 > 2.3. In current-clamp experiments, both 4AP and Ba2+ depolarised the membrane potential. In conclusion, rat brain endothelial cells express Kv1 and Kir2 K+ channels, both of which participate in setting membrane potential and could mediate K+ secretion into the brain interstitial fluid.
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google, scholar, article, cas, channels, pubmed, brain, cells, endothelial, potassium, kir, rat, channel, physiol, bloodbrain, barrier, expressed, biol, ion, wang, access, capillary, cell, regulation, cerebral, voltagegated, privacy, cookies, content, journal, millar, brown, hladky, conductance, expression, res, chem, popp, pharmacol, lond, choroid, plexus, publish, research, search, archiv, barrand, rectifying, membrane, sci,

Topics {✒️}

month download article/chapter depolarisation-activated delayed-rectifying outward voltage-gated potassium channels atp-sensitive cation channels vitro blood–brain barrier blood–brain-barrier—distribution blood–brain barrier methodology compromised blood–brain barrier outward-rectifying potassium channels calcium-activated potassium channels atp-sensitive cationic channels camp-dependent protein phosphorylation blood–brain barrier properties nervous system volume-sensitive chloride current time-independent inwardly rectifying brain endothelial cells mammalian potassium channel endothelial cells isolated full article pdf peripheral nervous systems small conductance ca2+-activated potassium channels contributes related subjects rat brain microvessels blood–brain barrier blood–brain-barrier potassium channel kv1 european economic area privacy choices/manage cookies brain capillary endothelium kir2 potassium channels cerebral capillary endothelium mammalian cell lines voltage-dependent fashion voltage-dependent gating blood–brain barriers intestinal epithelial cells porcine cerebral capillaries thyroid follicular cells brain interstitial fluid 5 cloned voltage-gated conditions privacy policy pig cerebral capillaries cell patch clamp enhanced cell proliferation resembled kir2 channels ion transport endothelial cells potassium channels

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         headline:Kv1 and Kir2 potassium channels are expressed in rat brain endothelial cells
         description:The endothelial cells of the brain microvasculature, which constitute the blood–brain barrier, secrete K+ into brain interstitial fluid. K+ channels are predicted to have a central role to play in this process. The aim of the following study was to characterise K+ channels in primary cultures of endothelial cells isolated from rat brain microvessels by whole-cell patch clamp and real-time polymerase chain reaction. In the 4 h after plating, the rat brain endothelial cells expressed predominantly a depolarisation-activated delayed-rectifying outward K+ conductance and a time-independent inwardly rectifying K+ conductance prominent at hyperpolarising potentials. The outward current was inhibited by 1 mM 4-aminopyridine (4AP), 10 nM margatoxin and 100 nM dendrotoxin-K, indicating the involvement of Kv1 channels. The half maximal activation voltage and time constants of activation and inactivation of the 4AP-sensitive current were similar to Kv1.3. The inwardly rectifying conductance was inhibited by Ba2+ in a dose- and voltage-dependent fashion; the kinetics of which resembled Kir2 channels. Quantification of messenger ribonucleic acid transcripts revealed Kv1.3 > 1.2 = 1.4 = 1.5 = 1.6 and Kir2.1 = 2 > 2.3. In current-clamp experiments, both 4AP and Ba2+ depolarised the membrane potential. In conclusion, rat brain endothelial cells express Kv1 and Kir2 K+ channels, both of which participate in setting membrane potential and could mediate K+ secretion into the brain interstitial fluid.
         datePublished:2007-11-20T00:00:00Z
         dateModified:2007-11-20T00:00:00Z
         pageStart:379
         pageEnd:391
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            Endothelial cells
            K channels
            Kv1
            Kir2
            Human Physiology
            Molecular Medicine
            Neurosciences
            Cell Biology
            Receptors
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      headline:Kv1 and Kir2 potassium channels are expressed in rat brain endothelial cells
      description:The endothelial cells of the brain microvasculature, which constitute the blood–brain barrier, secrete K+ into brain interstitial fluid. K+ channels are predicted to have a central role to play in this process. The aim of the following study was to characterise K+ channels in primary cultures of endothelial cells isolated from rat brain microvessels by whole-cell patch clamp and real-time polymerase chain reaction. In the 4 h after plating, the rat brain endothelial cells expressed predominantly a depolarisation-activated delayed-rectifying outward K+ conductance and a time-independent inwardly rectifying K+ conductance prominent at hyperpolarising potentials. The outward current was inhibited by 1 mM 4-aminopyridine (4AP), 10 nM margatoxin and 100 nM dendrotoxin-K, indicating the involvement of Kv1 channels. The half maximal activation voltage and time constants of activation and inactivation of the 4AP-sensitive current were similar to Kv1.3. The inwardly rectifying conductance was inhibited by Ba2+ in a dose- and voltage-dependent fashion; the kinetics of which resembled Kir2 channels. Quantification of messenger ribonucleic acid transcripts revealed Kv1.3 > 1.2 = 1.4 = 1.5 = 1.6 and Kir2.1 = 2 > 2.3. In current-clamp experiments, both 4AP and Ba2+ depolarised the membrane potential. In conclusion, rat brain endothelial cells express Kv1 and Kir2 K+ channels, both of which participate in setting membrane potential and could mediate K+ secretion into the brain interstitial fluid.
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         Endothelial cells
         K channels
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         Kir2
         Human Physiology
         Molecular Medicine
         Neurosciences
         Cell Biology
         Receptors
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