We are analyzing https://link.springer.com/article/10.1007/s00418-009-0662-5.

Title:
Cytokeratin expression during mouse embryonic and early postnatal mammary gland development | Histochemistry and Cell Biology
Description:
Cytokeratins are intermediate filament proteins found in most epithelial cells including the mammary epithelium. Specific cytokeratin expression has been found to mark different epithelial cell lineages and also to associate with putative mammary stem/progenitor cells. However, a comparative analysis of the expression of cytokaratins during embryonic and postnatal mammary development is currently lacking. Moreover, it is not clear whether the different classes of putative mammary stem/progenitor cells exist during embryonic development. Here, we use double/triple-label immunofluorescence and immunohistochemistry to systematically compare the expression of cytokeratin 5 (K5), cytokeratin 6 (K6), cytokeratin 8 (K8), cytokeratin 14 (K14) and cytokeratin 19 (K19) in embryonic and early postnatal mouse mammary glands. We show that K6+ and K8+/K14+ putative mammary progenitor cells arise during embryogenesis with distinct temporal and spatial distributions. Moreover, we describe a transient disconnection of the expression of K5 and K14, two cytokeratins that are often co-expressed, during the first postnatal weeks of mammary development. Finally, we report that cytokeratin expression in cultured primary mammary epithelial cells mimics that during the early stages of postnatal mammary development. These studies demonstrate an embryonic origin of putative mammary stem/progenitor cells. Moreover, they provide additional insights into the use of specific cytokeratins as markers of mammary epithelial differentiation, or the use of their promoters to direct gene overexpression or ablation in genetic studies of mouse mammary development.
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

cells, mammary, expression, development, cell, article, fig, pubmed, google, scholar, embryonic, cas, gland, glands, epithelial, postnatal, mouse, progenitor, min, immunofluorescence, primary, cytokeratin, stem, sections, found, suprabasalluminal, data, shown, cytokeratins, stemprogenitor, weeks, studies, putative, cultured, double, access, immunohistochemistry, differentiation, developmental, adult, colonies, antibodies, early, open, distinct, mice, sprout, compartment, positive, room,

Topics {✒️}

suprabasal/luminal k6/k14-coexpressing cells fitc-conjugated goat anti-rat rhodamine-conjugated goat anti-chicken reciprocal epithelial-mesenchymal interactions stem/progenitor cell-enriched populations k14+/k8+ suprabasal/luminal cells multipotent surface ectoderm mammary stem/progenitor cells pre-pubertal mammary epithelium biotinylated anti-rabbit igg article download pdf stem/progenitor cell origin lobulo-alveolar structures involute mammary basal/myoepithelial cells suprabasal/luminal k14+ cells putative multipotent/bipotent k6-expressing epithelial territories nih grants r01-ar47320 single stem cell mark basal/myoepithelial cells stem/progenitor cells mammary stem cells suprabasal/luminal cells mammary gland development multipotent progenitor cells early progenitor cells open access double/triple-label immunofluorescence stem cell units stem cell hierarchy mouse mammary gland mammogenic stem cells dormant stem cells mammary progenitor cells epithelial cell lineages epithelial cells including mammary epithelial differentiation developing mammary gland epithelial cells expressed early postnatal development mammary alveolar development functional mammary gland suprabasal/luminal compartment privacy choices/manage cookies postnatal mammary development single-lineage cells suprabasal/luminal k14+ postnatal mammary glands related subjects basal/myoepithelial cells

Questions {❓}

Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?

Schema {🗺️}

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         headline:Cytokeratin expression during mouse embryonic and early postnatal mammary gland development
         description:Cytokeratins are intermediate filament proteins found in most epithelial cells including the mammary epithelium. Specific cytokeratin expression has been found to mark different epithelial cell lineages and also to associate with putative mammary stem/progenitor cells. However, a comparative analysis of the expression of cytokaratins during embryonic and postnatal mammary development is currently lacking. Moreover, it is not clear whether the different classes of putative mammary stem/progenitor cells exist during embryonic development. Here, we use double/triple-label immunofluorescence and immunohistochemistry to systematically compare the expression of cytokeratin 5 (K5), cytokeratin 6 (K6), cytokeratin 8 (K8), cytokeratin 14 (K14) and cytokeratin 19 (K19) in embryonic and early postnatal mouse mammary glands. We show that K6+ and K8+/K14+ putative mammary progenitor cells arise during embryogenesis with distinct temporal and spatial distributions. Moreover, we describe a transient disconnection of the expression of K5 and K14, two cytokeratins that are often co-expressed, during the first postnatal weeks of mammary development. Finally, we report that cytokeratin expression in cultured primary mammary epithelial cells mimics that during the early stages of postnatal mammary development. These studies demonstrate an embryonic origin of putative mammary stem/progenitor cells. Moreover, they provide additional insights into the use of specific cytokeratins as markers of mammary epithelial differentiation, or the use of their promoters to direct gene overexpression or ablation in genetic studies of mouse mammary development.
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      headline:Cytokeratin expression during mouse embryonic and early postnatal mammary gland development
      description:Cytokeratins are intermediate filament proteins found in most epithelial cells including the mammary epithelium. Specific cytokeratin expression has been found to mark different epithelial cell lineages and also to associate with putative mammary stem/progenitor cells. However, a comparative analysis of the expression of cytokaratins during embryonic and postnatal mammary development is currently lacking. Moreover, it is not clear whether the different classes of putative mammary stem/progenitor cells exist during embryonic development. Here, we use double/triple-label immunofluorescence and immunohistochemistry to systematically compare the expression of cytokeratin 5 (K5), cytokeratin 6 (K6), cytokeratin 8 (K8), cytokeratin 14 (K14) and cytokeratin 19 (K19) in embryonic and early postnatal mouse mammary glands. We show that K6+ and K8+/K14+ putative mammary progenitor cells arise during embryogenesis with distinct temporal and spatial distributions. Moreover, we describe a transient disconnection of the expression of K5 and K14, two cytokeratins that are often co-expressed, during the first postnatal weeks of mammary development. Finally, we report that cytokeratin expression in cultured primary mammary epithelial cells mimics that during the early stages of postnatal mammary development. These studies demonstrate an embryonic origin of putative mammary stem/progenitor cells. Moreover, they provide additional insights into the use of specific cytokeratins as markers of mammary epithelial differentiation, or the use of their promoters to direct gene overexpression or ablation in genetic studies of mouse mammary development.
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