We are analyzing https://link.springer.com/article/10.1007/s00418-003-0566-8.

Title:
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes | Histochemistry and Cell Biology
Description:
Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-l-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate di-acetomethyl ester (DCDHF) and dihydrofluorescein diacetate (DHF). Mitochondria were counterstained with the potential-sensitive probe tetramethylrhodamine methyl ester perchlorate (TMRM). Both DCDHF and DHF were able to detect the presence of ROS in mitochondria, though with distinct morphological features. DCDHF fluorescence was invariably blurred, smudged, and spread over the cytoplasm surrounding the major mitochondrial clusters. On the contrary, DHF fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. These data suggest that DCDHF does not reach the mitochondrial matrix but is oxidized by ROS released by mitochondria in the cytosol. On the other hand, DHF enters mitochondria and reacts with ROS released in the matrix. Cytosolic (DCDHF+) ROS but not matrix (DHF+) ROS, were significantly decreased by vitamin E. N-acetyl-l-cysteine was effective in reducing DCDHF and DHF photooxidation in the medium, but was unable to reduce intracellular ROS. ROS generation was accompanied by partial mitochondrial depolarization.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,626,932 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

google, scholar, pubmed, cas, article, mitochondrial, mitochondria, oxygen, reactive, cell, ros, species, cells, biol, dcdhf, dhf, free, death, probes, diaz, liu, cambridge, privacy, cookies, content, isola, diacetate, access, production, university, press, apoptosis, superoxide, chem, biophys, cycle, data, publish, research, search, biology, dihydrofluorescein, diana, falchi, fluorescent, membrane, potential, oxidative, related, radical,

Topics {✒️}

month download article/chapter multidrug resistance p-glycoprotein time-dependent membrane potentials n-acetyl-l-cysteine ros-sensitive probes 6-carboxy-2′ fluorescent dye 2′-7′-dichlorofluorescein protective nuclear responses redox-sensitive fluorophores full article pdf cell biology aims privacy choices/manage cookies sensitive transcription factors glutathione redox equilibrium cervical carcinoma cells chemilumigenic probes lucigenin mitochondrial transmembrane potential reactive oxygen species ceramide-dependent apoptosis respiratory chain-superoxide generating mitochondrial membrane potential hela cells exposed retinamide-induced apoptosis oxidative stress measurements safely low levels free radical formation major mitochondrial clusters partial mitochondrial depolarization tmrm-stained mitochondria article diaz fluorescent indicators related subjects mitochondrial permeability transition european economic area tert-butyl hydroperoxide distinct morphological features delineated thin filaments drosophila neuron subtypes homogenous longitudinal profiles o'malley yq rhodamine dyes assessorato dell'igiene dell'assistenza sociale apoptotic cell death brain respiratory chain article histochemistry 'reactive oxygen cycle' reactive oxygen cycle conditions privacy policy superoxide anion radical permeability pore transition

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes
         description:Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-l-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate di-acetomethyl ester (DCDHF) and dihydrofluorescein diacetate (DHF). Mitochondria were counterstained with the potential-sensitive probe tetramethylrhodamine methyl ester perchlorate (TMRM). Both DCDHF and DHF were able to detect the presence of ROS in mitochondria, though with distinct morphological features. DCDHF fluorescence was invariably blurred, smudged, and spread over the cytoplasm surrounding the major mitochondrial clusters. On the contrary, DHF fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. These data suggest that DCDHF does not reach the mitochondrial matrix but is oxidized by ROS released by mitochondria in the cytosol. On the other hand, DHF enters mitochondria and reacts with ROS released in the matrix. Cytosolic (DCDHF+) ROS but not matrix (DHF+) ROS, were significantly decreased by vitamin E. N-acetyl-l-cysteine was effective in reducing DCDHF and DHF photooxidation in the medium, but was unable to reduce intracellular ROS. ROS generation was accompanied by partial mitochondrial depolarization.
         datePublished:2003-09-20T00:00:00Z
         dateModified:2003-09-20T00:00:00Z
         pageStart:319
         pageEnd:325
         sameAs:https://doi.org/10.1007/s00418-003-0566-8
         keywords:
            Mitochondria
            ROS
            TMRM
            DHF
            DCDHF
            Biomedicine
            general
            Cell Biology
            Biochemistry
            Developmental Biology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb1.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8flb2.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb3a-b.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb4.jpg
         isPartOf:
            name:Histochemistry and Cell Biology
            issn:
               1432-119X
               0948-6143
            volumeNumber:120
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Giacomo Diaz
               affiliation:
                     name:University of Cagliari
                     address:
                        name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Shusen Liu
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Raffaella Isola
               affiliation:
                     name:University of Cagliari
                     address:
                        name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Andrea Diana
               affiliation:
                     name:University of Cagliari
                     address:
                        name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Angela Maria Falchi
               affiliation:
                     name:University of Cagliari
                     address:
                        name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes
      description:Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-l-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate di-acetomethyl ester (DCDHF) and dihydrofluorescein diacetate (DHF). Mitochondria were counterstained with the potential-sensitive probe tetramethylrhodamine methyl ester perchlorate (TMRM). Both DCDHF and DHF were able to detect the presence of ROS in mitochondria, though with distinct morphological features. DCDHF fluorescence was invariably blurred, smudged, and spread over the cytoplasm surrounding the major mitochondrial clusters. On the contrary, DHF fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. These data suggest that DCDHF does not reach the mitochondrial matrix but is oxidized by ROS released by mitochondria in the cytosol. On the other hand, DHF enters mitochondria and reacts with ROS released in the matrix. Cytosolic (DCDHF+) ROS but not matrix (DHF+) ROS, were significantly decreased by vitamin E. N-acetyl-l-cysteine was effective in reducing DCDHF and DHF photooxidation in the medium, but was unable to reduce intracellular ROS. ROS generation was accompanied by partial mitochondrial depolarization.
      datePublished:2003-09-20T00:00:00Z
      dateModified:2003-09-20T00:00:00Z
      pageStart:319
      pageEnd:325
      sameAs:https://doi.org/10.1007/s00418-003-0566-8
      keywords:
         Mitochondria
         ROS
         TMRM
         DHF
         DCDHF
         Biomedicine
         general
         Cell Biology
         Biochemistry
         Developmental Biology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb1.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8flb2.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb3a-b.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00418-003-0566-8/MediaObjects/s00418-003-0566-8fhb4.jpg
      isPartOf:
         name:Histochemistry and Cell Biology
         issn:
            1432-119X
            0948-6143
         volumeNumber:120
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Giacomo Diaz
            affiliation:
                  name:University of Cagliari
                  address:
                     name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Shusen Liu
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Raffaella Isola
            affiliation:
                  name:University of Cagliari
                  address:
                     name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Andrea Diana
            affiliation:
                  name:University of Cagliari
                  address:
                     name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Angela Maria Falchi
            affiliation:
                  name:University of Cagliari
                  address:
                     name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Histochemistry and Cell Biology
      issn:
         1432-119X
         0948-6143
      volumeNumber:120
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Cagliari
      address:
         name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:University of Cagliari
      address:
         name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
         type:PostalAddress
      name:University of Cagliari
      address:
         name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
         type:PostalAddress
      name:University of Cagliari
      address:
         name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Giacomo Diaz
      affiliation:
            name:University of Cagliari
            address:
               name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Shusen Liu
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Raffaella Isola
      affiliation:
            name:University of Cagliari
            address:
               name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
               type:PostalAddress
            type:Organization
      name:Andrea Diana
      affiliation:
            name:University of Cagliari
            address:
               name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
               type:PostalAddress
            type:Organization
      name:Angela Maria Falchi
      affiliation:
            name:University of Cagliari
            address:
               name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
      name:National Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
      name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
      name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
      name:Department of Cytomorphology, University of Cagliari, Monserrato (CA), Italy
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(117)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.81s.

LINK . SPRINGER . COM {}