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Title:
Multi-slice computed tomography with N1177 identifies ruptured atherosclerotic plaques in rabbits | Basic Research in Cardiology
Description:
Rupture-prone and ruptured plaques are characterized by the presence of large numbers of macrophages. N1177 is a contrast agent consisting of iodinated nanoparticles that are selectively phagocytosed by macrophages. The aim of this study was to investigate the effect of N1177 on the CT attenuation of rupture-prone and ruptured plaques in rabbits. In addition, we examined in vitro whether uptake of N1177 occurred without cytotoxic or pro-inflammatory effects on macrophages. In vitro, the viability of J774 macrophages was not affected by treatment with N1177. Moreover, N1177 had no effect on the phagocytic capacity or cytokine production of macrophages. For the in vivo experiments, 6 New Zealand White rabbits were fed a cholesterol-supplemented diet for 12–15 months, resulting in the development of large atherosclerotic plaques that resembled rupture-prone plaques in humans. In three rabbits, mechanical plaque rupture was induced by retrograde pullback of an embolic protection device. N1177 had no effect on the median density of rupture-prone plaques [35 HU (range 3–85) before injection vs. 32 HU (range 1–93) 2 h after injection of N1177; P > 0.05]. However, after induction of mechanical plaque rupture, the median density of the atherosclerotic plaques increased from 40 HU (range 6–86) before injection to 74 HU (range 14–111) 2 h after injection of N1177 (P < 0.001). Using time-of-flight static secondary ion mass spectrometry, the presence of N1177 nanoparticles was demonstrated in macrophage-rich areas of ruptured plaques, but not of non-ruptured plaques. In conclusion, our results show that N1177 is a contrast agent that can identify ruptured atherosclerotic plaques.
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google, scholar, article, pubmed, coronary, cas, van, computed, atherosclerotic, tomography, plaques, cardiol, plaque, imaging, macrophages, basic, meyer, res, coll, rabbits, herman, lesions, research, martinet, atherosclerosis, human, cardiovasc, slice, acute, university, antwerp, multislice, ruptured, study, vivo, rupture, access, becker, characterization, cardiovascular, circulation, intravascular, ultrasound, division, belgium, privacy, cookies, content, herck, bult,
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month download article/chapter multi-slice computed tomography multi-detector-row ct multi-detector row ct dual-energy computed tomography macrophage-rich atherosclerotic plaque luc van vaeck related subjects u-king-im j roel de mondt resembled rupture-prone plaques jozef van herck article basic research coronary artery disease 64-slice computed tomography iron oxide-enhanced mri rupture-prone plaques [35 hu full article pdf acute coronary syndromes multidetector computed tomography van der pluijm quantitative coronary angiography coronary artery stenosis conventional coronary angiography privacy choices/manage cookies submillimeter computed tomography characterize atherosclerotic plaque macrophage-rich areas van de ruit coronary atherosclerotic plaques cardiac computed tomography disease biology affecting mechanical plaque rupture acute coronary syndrome human atherosclerotic plaques de meyer gry van engelshoven jma van mieghem cag van hove ce cor van hove successful molecular imaging vulnerable nonstenotic plaque quantify plaque volumes human coronary arteries annick ludwig noncalcified coronary plaques atherosclerotic plaques increased proximal coronary system coronary vessel evoked sudden coronary death
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headline:Multi-slice computed tomography with N1177 identifies ruptured atherosclerotic plaques in rabbits
description:Rupture-prone and ruptured plaques are characterized by the presence of large numbers of macrophages. N1177 is a contrast agent consisting of iodinated nanoparticles that are selectively phagocytosed by macrophages. The aim of this study was to investigate the effect of N1177 on the CT attenuation of rupture-prone and ruptured plaques in rabbits. In addition, we examined in vitro whether uptake of N1177 occurred without cytotoxic or pro-inflammatory effects on macrophages. In vitro, the viability of J774 macrophages was not affected by treatment with N1177. Moreover, N1177 had no effect on the phagocytic capacity or cytokine production of macrophages. For the in vivo experiments, 6 New Zealand White rabbits were fed a cholesterol-supplemented diet for 12–15 months, resulting in the development of large atherosclerotic plaques that resembled rupture-prone plaques in humans. In three rabbits, mechanical plaque rupture was induced by retrograde pullback of an embolic protection device. N1177 had no effect on the median density of rupture-prone plaques [35 HU (range 3–85) before injection vs. 32 HU (range 1–93) 2 h after injection of N1177; P > 0.05]. However, after induction of mechanical plaque rupture, the median density of the atherosclerotic plaques increased from 40 HU (range 6–86) before injection to 74 HU (range 14–111) 2 h after injection of N1177 (P < 0.001). Using time-of-flight static secondary ion mass spectrometry, the presence of N1177 nanoparticles was demonstrated in macrophage-rich areas of ruptured plaques, but not of non-ruptured plaques. In conclusion, our results show that N1177 is a contrast agent that can identify ruptured atherosclerotic plaques.
datePublished:2009-08-20T00:00:00Z
dateModified:2009-08-20T00:00:00Z
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Multi-slice computed tomography
N1177
Molecular imaging
Macrophage
Cardiology
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headline:Multi-slice computed tomography with N1177 identifies ruptured atherosclerotic plaques in rabbits
description:Rupture-prone and ruptured plaques are characterized by the presence of large numbers of macrophages. N1177 is a contrast agent consisting of iodinated nanoparticles that are selectively phagocytosed by macrophages. The aim of this study was to investigate the effect of N1177 on the CT attenuation of rupture-prone and ruptured plaques in rabbits. In addition, we examined in vitro whether uptake of N1177 occurred without cytotoxic or pro-inflammatory effects on macrophages. In vitro, the viability of J774 macrophages was not affected by treatment with N1177. Moreover, N1177 had no effect on the phagocytic capacity or cytokine production of macrophages. For the in vivo experiments, 6 New Zealand White rabbits were fed a cholesterol-supplemented diet for 12–15 months, resulting in the development of large atherosclerotic plaques that resembled rupture-prone plaques in humans. In three rabbits, mechanical plaque rupture was induced by retrograde pullback of an embolic protection device. N1177 had no effect on the median density of rupture-prone plaques [35 HU (range 3–85) before injection vs. 32 HU (range 1–93) 2 h after injection of N1177; P > 0.05]. However, after induction of mechanical plaque rupture, the median density of the atherosclerotic plaques increased from 40 HU (range 6–86) before injection to 74 HU (range 14–111) 2 h after injection of N1177 (P < 0.001). Using time-of-flight static secondary ion mass spectrometry, the presence of N1177 nanoparticles was demonstrated in macrophage-rich areas of ruptured plaques, but not of non-ruptured plaques. In conclusion, our results show that N1177 is a contrast agent that can identify ruptured atherosclerotic plaques.
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Multi-slice computed tomography
N1177
Molecular imaging
Macrophage
Cardiology
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