We are analyzing https://link.springer.com/article/10.1007/s00395-005-0566-z.

Title:
Localizing extracellular signal–regulated kinase (ERK) in pharmacological preconditioning's trigger pathway | Basic Research in Cardiology
Description:
Acetylcholine (ACh) and opioid receptor agonists trigger the preconditioned phenotype through sequential activation of the epidermal growth factor (EGF) receptor, phosphatidylinositol 3-kinase (PI3-K), Akt, and nitric oxide synthase (NOS), and opening of mitochondrial (mito) KATP channels with the generation of reactive oxygen species (ROS). Although extracellular signal–regulated kinase (ERK) has recently been reported to be part of this pathway, its location has not been determined. To address this issue, we administered a 5–min pulse of ACh (550 µM) prior to 30 min of ischemia in isolated rabbit hearts. It reduced infarction from 30.4 ± 2.2% of the risk zone in control hearts to 12.3 ± 2.8% and co–administration of the MEK, and, therefore, downstream ERK inhibitor U0126 abolished protection (29.1 ± 4.6% infarction) con.rming ERK
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

While profit motivates many websites, others exist to inspire, entertain, or provide valuable resources. Websites have a variety of goals. And this might be one of them. Link.springer.com might have a hidden revenue stream, but it's not something we can detect.

Keywords {🔍}

google, scholar, cas, article, downey, cohen, pubmed, erk, ros, kinase, res, critz, mitochondrial, production, preconditioning, philipp, opening, physiol, research, acetylcholine, receptor, activation, akt, nos, katp, channels, signalling, privacy, cookies, content, basic, extracellular, trigger, pathway, cui, ach, rabbit, bradykinin, access, cardiovasc, cardiol, qin, alabama, data, publish, search, signalregulated, egf, generation, hearts,

Topics {✒️}

met5-enkephalin-induced cardioprotection occurs extracellular signal–regulated kinase month download article/chapter δ1-opioid receptor-induced cardioprotection δ-opioid agonist dadle article basic research adenosine activate pi3-kinase nitric oxide synthase full article pdf privacy choices/manage cookies mitokatp channel opening u0126 blocked phosphorylation preconditioning- induced protection adult rabbit cardiomyocytes mek inhibitors u0126 related subjects bradykinin-induced preconditioning placing erk downstream measuring ros production generating mitochondrial ros mitochondrial katp channels increased ros production mitochondrial ros generation tyrosine kinase-dependent european economic area scope submit manuscript epidermal growth factor reactive oxygen species atpsensitive potassium channels bradykinin trigger preconditioning 4-dihydroxybenzoate protect myocardium receptor tyrosine kinases conditions privacy policy isolated rabbit cardiomyocytes adenosine antagonist spt generating free radicals inhibitor wortmannin blocked src-kinase activation egfr tyrosine kinase protein–coupled agonists placing erk upstream myocardial infarction van winkle dm pro-survival kinases activating prosurvival kinases accepting optional cookies protein kinase akt acetylcholineinduced production gi proteins isolated rabbit hearts

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Localizing extracellular signal–regulated kinase (ERK) in pharmacological preconditioning's trigger pathway
         description:Acetylcholine (ACh) and opioid receptor agonists trigger the preconditioned phenotype through sequential activation of the epidermal growth factor (EGF) receptor, phosphatidylinositol 3-kinase (PI3-K), Akt, and nitric oxide synthase (NOS), and opening of mitochondrial (mito) KATP channels with the generation of reactive oxygen species (ROS). Although extracellular signal–regulated kinase (ERK) has recently been reported to be part of this pathway, its location has not been determined. To address this issue, we administered a 5–min pulse of ACh (550 µM) prior to 30 min of ischemia in isolated rabbit hearts. It reduced infarction from 30.4 ± 2.2% of the risk zone in control hearts to 12.3 ± 2.8% and co–administration of the MEK, and, therefore, downstream ERK inhibitor U0126 abolished protection (29.1 ± 4.6% infarction) con.rming ERK's involvement. MitoKATP opening was monitored in adult rabbit cardiomyocytes by measuring ROS production with MitoTracker Red. ROS production was increased by each of three G protein–coupled agonists: ACh (250 µM), bradykinin (BK) (500 nM), and the δ-opioid agonist DADLE (20 nM). Co–incubation with the MEK inhibitors U0126 (500 nM) or PD 98059 (10 µM) blocked the increased ROS production seen with all three agonists. Direct activation of its receptor by EGF increased ROS production and PD 98059 blocked that increase, thus placing ERK downstream of the EGF receptor. Desferoxamine (DFO) which opens mitoKATP through direct activation of NOS also increased ROS. PD 98059 could not block DFO–induced ROS production, placing ERK upstream of NOS. In isolated hearts, ACh caused phosphorylation of both Akt and ERK. U0126 blocked phosphorylation of ERK but not of Akt. The PI3–K inhibitor wortmannin blocked both. Together these data indicate that ERK is located between Akt and NOS.
         datePublished:2005-11-11T00:00:00Z
         dateModified:2005-11-11T00:00:00Z
         pageStart:159
         pageEnd:167
         sameAs:https://doi.org/10.1007/s00395-005-0566-z
         keywords:
            acetylcholine
            bradykinin
            DADLE
            ERK
            U0126
            Cardiology
         image:
         isPartOf:
            name:Basic Research in Cardiology
            issn:
               1435-1803
               0300-8428
            volumeNumber:101
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Steinkopff-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:S. Philipp
               affiliation:
                     name:University of South Alabama College of Medicine
                     address:
                        name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                        type:PostalAddress
                     type:Organization
                     name:West German Heart Center Essen, University of Duisberg-Essen
                     address:
                        name:Department of Cardiology, West German Heart Center Essen, University of Duisberg-Essen, Essen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:St. D. Critz
               affiliation:
                     name:University of South Alabama
                     address:
                        name:Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:L. Cui
               affiliation:
                     name:University of South Alabama College of Medicine
                     address:
                        name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:V. Solodushko
               affiliation:
                     name:University of South Alabama College of Medicine
                     address:
                        name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:M. V. Cohen
               affiliation:
                     name:University of South Alabama College of Medicine
                     address:
                        name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                        type:PostalAddress
                     type:Organization
                     name:College of Medicine, University of South Alabama
                     address:
                        name:Departments of Physiology and Medicine, College of Medicine, University of South Alabama, Mobile, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:J. M. Downey
               affiliation:
                     name:University of South Alabama College of Medicine
                     address:
                        name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Localizing extracellular signal–regulated kinase (ERK) in pharmacological preconditioning's trigger pathway
      description:Acetylcholine (ACh) and opioid receptor agonists trigger the preconditioned phenotype through sequential activation of the epidermal growth factor (EGF) receptor, phosphatidylinositol 3-kinase (PI3-K), Akt, and nitric oxide synthase (NOS), and opening of mitochondrial (mito) KATP channels with the generation of reactive oxygen species (ROS). Although extracellular signal–regulated kinase (ERK) has recently been reported to be part of this pathway, its location has not been determined. To address this issue, we administered a 5–min pulse of ACh (550 µM) prior to 30 min of ischemia in isolated rabbit hearts. It reduced infarction from 30.4 ± 2.2% of the risk zone in control hearts to 12.3 ± 2.8% and co–administration of the MEK, and, therefore, downstream ERK inhibitor U0126 abolished protection (29.1 ± 4.6% infarction) con.rming ERK's involvement. MitoKATP opening was monitored in adult rabbit cardiomyocytes by measuring ROS production with MitoTracker Red. ROS production was increased by each of three G protein–coupled agonists: ACh (250 µM), bradykinin (BK) (500 nM), and the δ-opioid agonist DADLE (20 nM). Co–incubation with the MEK inhibitors U0126 (500 nM) or PD 98059 (10 µM) blocked the increased ROS production seen with all three agonists. Direct activation of its receptor by EGF increased ROS production and PD 98059 blocked that increase, thus placing ERK downstream of the EGF receptor. Desferoxamine (DFO) which opens mitoKATP through direct activation of NOS also increased ROS. PD 98059 could not block DFO–induced ROS production, placing ERK upstream of NOS. In isolated hearts, ACh caused phosphorylation of both Akt and ERK. U0126 blocked phosphorylation of ERK but not of Akt. The PI3–K inhibitor wortmannin blocked both. Together these data indicate that ERK is located between Akt and NOS.
      datePublished:2005-11-11T00:00:00Z
      dateModified:2005-11-11T00:00:00Z
      pageStart:159
      pageEnd:167
      sameAs:https://doi.org/10.1007/s00395-005-0566-z
      keywords:
         acetylcholine
         bradykinin
         DADLE
         ERK
         U0126
         Cardiology
      image:
      isPartOf:
         name:Basic Research in Cardiology
         issn:
            1435-1803
            0300-8428
         volumeNumber:101
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Steinkopff-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:S. Philipp
            affiliation:
                  name:University of South Alabama College of Medicine
                  address:
                     name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                     type:PostalAddress
                  type:Organization
                  name:West German Heart Center Essen, University of Duisberg-Essen
                  address:
                     name:Department of Cardiology, West German Heart Center Essen, University of Duisberg-Essen, Essen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:St. D. Critz
            affiliation:
                  name:University of South Alabama
                  address:
                     name:Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:L. Cui
            affiliation:
                  name:University of South Alabama College of Medicine
                  address:
                     name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:V. Solodushko
            affiliation:
                  name:University of South Alabama College of Medicine
                  address:
                     name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M. V. Cohen
            affiliation:
                  name:University of South Alabama College of Medicine
                  address:
                     name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                     type:PostalAddress
                  type:Organization
                  name:College of Medicine, University of South Alabama
                  address:
                     name:Departments of Physiology and Medicine, College of Medicine, University of South Alabama, Mobile, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. M. Downey
            affiliation:
                  name:University of South Alabama College of Medicine
                  address:
                     name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Basic Research in Cardiology
      issn:
         1435-1803
         0300-8428
      volumeNumber:101
Organization:
      name:Steinkopff-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of South Alabama College of Medicine
      address:
         name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
         type:PostalAddress
      name:West German Heart Center Essen, University of Duisberg-Essen
      address:
         name:Department of Cardiology, West German Heart Center Essen, University of Duisberg-Essen, Essen, Germany
         type:PostalAddress
      name:University of South Alabama
      address:
         name:Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, USA
         type:PostalAddress
      name:University of South Alabama College of Medicine
      address:
         name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
         type:PostalAddress
      name:University of South Alabama College of Medicine
      address:
         name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
         type:PostalAddress
      name:University of South Alabama College of Medicine
      address:
         name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
         type:PostalAddress
      name:College of Medicine, University of South Alabama
      address:
         name:Departments of Physiology and Medicine, College of Medicine, University of South Alabama, Mobile, USA
         type:PostalAddress
      name:University of South Alabama College of Medicine
      address:
         name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:S. Philipp
      affiliation:
            name:University of South Alabama College of Medicine
            address:
               name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
               type:PostalAddress
            type:Organization
            name:West German Heart Center Essen, University of Duisberg-Essen
            address:
               name:Department of Cardiology, West German Heart Center Essen, University of Duisberg-Essen, Essen, Germany
               type:PostalAddress
            type:Organization
      name:St. D. Critz
      affiliation:
            name:University of South Alabama
            address:
               name:Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, USA
               type:PostalAddress
            type:Organization
      name:L. Cui
      affiliation:
            name:University of South Alabama College of Medicine
            address:
               name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
               type:PostalAddress
            type:Organization
      name:V. Solodushko
      affiliation:
            name:University of South Alabama College of Medicine
            address:
               name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
               type:PostalAddress
            type:Organization
      name:M. V. Cohen
      affiliation:
            name:University of South Alabama College of Medicine
            address:
               name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
               type:PostalAddress
            type:Organization
            name:College of Medicine, University of South Alabama
            address:
               name:Departments of Physiology and Medicine, College of Medicine, University of South Alabama, Mobile, USA
               type:PostalAddress
            type:Organization
      name:J. M. Downey
      affiliation:
            name:University of South Alabama College of Medicine
            address:
               name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
      name:Department of Cardiology, West German Heart Center Essen, University of Duisberg-Essen, Essen, Germany
      name:Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, USA
      name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
      name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
      name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
      name:Departments of Physiology and Medicine, College of Medicine, University of South Alabama, Mobile, USA
      name:Department of Physiology MSB 3074, University of South Alabama College of Medicine, Mobile, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(78)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.23s.

LINK . SPRINGER . COM {}