We are analyzing https://link.springer.com/article/10.1007/s003940070028.

Title:
Short-chain fatty acid (SCFA) uptake into Caco-2 cells by a pH-dependent and carrier mediated transport mechanism | European Journal of Nutrition
Description:
The short-chain fatty acids, acetate, propionate, and butyrate, are the most abundant organic anions in the human colon. SCFA play a pivotal role in maintaining homeostasis in the colon. Particularly butyrate induces cell differentiation and regulates growth and proliferation of colonic mucosal epithelial cells, whereas it reduces the growth rate of colorectal cancer cell. Previous studies by several groups, including our own, using isolated membrane vesicles have demonstrated that the uptake of butyrate is at least in part mediated by a non-electrogenic SCFA−/HCO3 − antiporter. The purpose of the present study was to determine (1) whether Caco-2 cells could serve as an experimental model to assess the mechanisms of SCFA transport, and (2) whether monocarboxylate transporters could play a role in SCFA transport in these cells. Caco-2 cells were found to transport 14C-butyrate in a concentration and time dependent manner. The uptake was sodium independent, but was stimulated by lowering extracellular pH. The uptake of 500μM butyrate was reduced by 49.6% ± 3.3% in the presence of propionate and by 57.2% ± 4.8% in the presence of 10 mM L-lactate. The addition of 1 mM α-cyano-4-hydroxycinnamate and phloretin, both known to be potent inhibitors of MCT1, decreased the uptake of 500 μM 14C-butyrate by 59.4% ± 4.1% and 48.9% ± 3.3%, respectively, whereas similar concentrations of DIDS did not have any effect. These data suggest that the uptake of butyrate in Caco-2 cells occurs via a carrier mediated transport system specific for monocarboxylic acids, which is in accordance with characteristics of the MCT1.
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Keywords {🔍}

article, transport, cells, uptake, butyrate, fatty, caco, access, privacy, cookies, content, journal, shortchain, scfa, mediated, acids, data, information, publish, search, carrier, colon, european, log, research, stein, zores, schröder, play, role, open, discover, springer, optional, personal, including, parties, policy, find, track, nutrition, acid, phdependent, mechanism, cite, explore, propionate, cell, growth, colorectal,

Topics {✒️}

short-chain fatty acids short-chain fatty acid colorectal cancer cell month download article/chapter johann wolfgang goethe-university 1 mm α-cyano-4-hydroxycinnamate isolated membrane vesicles related subjects article european journal transport 14c-butyrate privacy choices/manage cookies full article pdf caco-2 cells occurs european economic area 10 mm l-lactate check access instant access 500 μm 14c-butyrate scope submit manuscript abundant organic anions monocarboxylate transporters theodor-stern-kai 7 conditions privacy policy monocarboxylic acids time dependent manner lowering extracellular ph journal finder publish accepting optional cookies part mediated main content log scfa transport caco-2 cells scfa play human colon article log article cite article stein nutrition aims privacy policy personal data books a journal publish 500μm butyrate optional cookies manage preferences play colon ph-dependent essential cookies cookies skip

Schema {🗺️}

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         description: The short-chain fatty acids, acetate, propionate, and butyrate, are the most abundant organic anions in the human colon. SCFA play a pivotal role in maintaining homeostasis in the colon. Particularly butyrate induces cell differentiation and regulates growth and proliferation of colonic mucosal epithelial cells, whereas it reduces the growth rate of colorectal cancer cell. Previous studies by several groups, including our own, using isolated membrane vesicles have demonstrated that the uptake of butyrate is at least in part mediated by a non-electrogenic SCFA−/HCO3 − antiporter. The purpose of the present study was to determine (1) whether Caco-2 cells could serve as an experimental model to assess the mechanisms of SCFA transport, and (2) whether monocarboxylate transporters could play a role in SCFA transport in these cells. Caco-2 cells were found to transport 14C-butyrate in a concentration and time dependent manner. The uptake was sodium independent, but was stimulated by lowering extracellular pH. The uptake of 500μM butyrate was reduced by 49.6% ± 3.3% in the presence of propionate and by 57.2% ± 4.8% in the presence of 10 mM L-lactate. The addition of 1 mM α-cyano-4-hydroxycinnamate and phloretin, both known to be potent inhibitors of MCT1, decreased the uptake of 500 μM 14C-butyrate by 59.4% ± 4.1% and 48.9% ± 3.3%, respectively, whereas similar concentrations of DIDS did not have any effect. These data suggest that the uptake of butyrate in Caco-2 cells occurs via a carrier mediated transport system specific for monocarboxylic acids, which is in accordance with characteristics of the MCT1.
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      description: The short-chain fatty acids, acetate, propionate, and butyrate, are the most abundant organic anions in the human colon. SCFA play a pivotal role in maintaining homeostasis in the colon. Particularly butyrate induces cell differentiation and regulates growth and proliferation of colonic mucosal epithelial cells, whereas it reduces the growth rate of colorectal cancer cell. Previous studies by several groups, including our own, using isolated membrane vesicles have demonstrated that the uptake of butyrate is at least in part mediated by a non-electrogenic SCFA−/HCO3 − antiporter. The purpose of the present study was to determine (1) whether Caco-2 cells could serve as an experimental model to assess the mechanisms of SCFA transport, and (2) whether monocarboxylate transporters could play a role in SCFA transport in these cells. Caco-2 cells were found to transport 14C-butyrate in a concentration and time dependent manner. The uptake was sodium independent, but was stimulated by lowering extracellular pH. The uptake of 500μM butyrate was reduced by 49.6% ± 3.3% in the presence of propionate and by 57.2% ± 4.8% in the presence of 10 mM L-lactate. The addition of 1 mM α-cyano-4-hydroxycinnamate and phloretin, both known to be potent inhibitors of MCT1, decreased the uptake of 500 μM 14C-butyrate by 59.4% ± 4.1% and 48.9% ± 3.3%, respectively, whereas similar concentrations of DIDS did not have any effect. These data suggest that the uptake of butyrate in Caco-2 cells occurs via a carrier mediated transport system specific for monocarboxylic acids, which is in accordance with characteristics of the MCT1.
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