We are analyzing https://link.springer.com/article/10.1007/s002849900320.

Title:
The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens | Current Microbiology
Description:
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-β-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with newly constructed strains containing inserts without the lacY gene (lacIPOZ). The lactose operon inserts were introduced as single-copy chromosomal inserts to eliminate differences in expression caused by differences in copy number. Comparison between the two types of inserts showed that the lactose permease was essential to allow growth on lactose by both bacteria and that the lactose permease plays an important role in transporting the inducer IPTG across the membrane of P. fluorescens. The use of a functional lactose permease allows expression of β-galactosidase to increase more than fivefold from a wild-type lac promoter in P. fluorescens SS1001. We suggest that an increase in the rate of protein synthesis from lac-type promoters could be enhanced if an active lactose permease is present as well.
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Keywords {🔍}

article, lactose, gene, coli, privacy, cookies, content, iptg, escherichia, fluorescens, permease, access, information, publish, search, lacy, promoters, inserts, bacterial, data, log, journal, research, microbiology, effect, induction, inducible, studied, pseudomonas, hansen, knudsen, sørensen, expression, protein, discover, springer, essential, optional, personal, parties, policy, find, track, current, cite, lars, steen, søren, explore, role,

Topics {✒️}

isopropyl-β-d-thiogalactopyranoside month download article/chapter wild-type lac promoter bacterial chemical protein synthesis single-copy chromosomal inserts escherichia coli bl21 privacy choices/manage cookies full article pdf pseudomonas fluorescens published related subjects lacipozya chromosomal insert lac-type promoters lactose permease plays functional lactose permease active lactose permease european economic area lacy gene β-galactosidase conditions privacy policy escherichia coli newly constructed strains lactose operon inserts steen knudsen & søren check access instant access accepting optional cookies main content log iptg inducible promoters journal finder publish pseudomonas fluorescens article log general microbiology lactose permease article cite article hansen privacy policy personal data lac operon books a inducible promoters expression caused information optional cookies manage preferences copy number dk lars data protection cookies skip subscription content

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