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Metagenomic Analysis of the Jinding Duck Fecal Virome | Current Microbiology
Description:
Ducks play an important role in transmitting and maintaining mammalian viruses in nature, and are a reservoir host of many animal viruses. We analyzed the fecal virome of four strains (A, B, C, and D) of ducks living in isolation by using metagenomic analysis. The feces of the ducks tested contained 18 animal virus families. The percentage values of RNA virus reads, compared to the total animal virus reads in each of the four strains were 96.96% (A), 97.30% (B), 98.01 (C), and 67.49% (D), and were mainly from Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Reoviridae. Meanwhile, the minority of DNA virus reads were related to Herpesviridae, Adenoviridae, Iridoviridae, and other, low abundance viral families. The percentage values of Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Herpesviridae reads were not significantly different among strains A, B, and C; however, there were marked differences in the abundance of these reads in strain D. In summary, this study provides an unbiased examination of the viral diversity in the feces of four strains of ducks in specific-pathogen-free periods, and highlights the variation in the percentage of viral families present. These results can be used as a reference for detecting duck viral pathogens and predicting zoonotic potential.
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pubmed, article, google, scholar, cas, virus, central, viral, analysis, wang, viruses, avian, duck, influenza, virol, metagenomic, ducks, access, research, virome, animal, diversity, delwart, privacy, cookies, content, fecal, chen, reads, gut, open, wild, publish, search, zhao, niu, strains, feces, veterinary, identification, microbiol, author, data, information, log, journal, microbiology, january, lili, yinjie,
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month download article/chapter yiping wangĀ &Ā hongyan chen specific-pathogen-free periods metagenomics rast server full article pdf 1007/s00284-018-1430-3 access full-length genome characterization privacy choices/manage cookies viral families present uncultured viral community duck enteritis virus duck virus enteritis high-density farm article zhao related subjects kuttanad duck embryos fecal viral flora bat guano virome bovine parvovirus species viral shedding patterns rna virus reads hongyan chen disease virus dna mouth disease virus virus population present uncharacterized virus genomes single-primer-amplification stallknecht de avian rna viruses dna virus reads european economic area developmental ossification sequences anas platyrhynchos domesticus acute flaccid paralysis victoria jg chicken embryo fibroblasts type asia1 foot sequence-independent california sea lions innate immune responses wild pigeon droppings conditions privacy policy avian influenza viruses article log predicting zoonotic potential ethics declarations conflict pantin-jackwood mj open vet accepting optional cookies chen jm
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headline:Metagenomic Analysis of the Jinding Duck Fecal Virome
description:Ducks play an important role in transmitting and maintaining mammalian viruses in nature, and are a reservoir host of many animal viruses. We analyzed the fecal virome of four strains (A, B, C, and D) of ducks living in isolation by using metagenomic analysis. The feces of the ducks tested contained 18 animal virus families. The percentage values of RNA virus reads, compared to the total animal virus reads in each of the four strains were 96.96% (A), 97.30% (B), 98.01 (C), and 67.49% (D), and were mainly from Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Reoviridae. Meanwhile, the minority of DNA virus reads were related to Herpesviridae, Adenoviridae, Iridoviridae, and other, low abundance viral families. The percentage values of Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Herpesviridae reads were not significantly different among strains A, B, and C; however, there were marked differences in the abundance of these reads in strain D. In summary, this study provides an unbiased examination of the viral diversity in the feces of four strains of ducks in specific-pathogen-free periods, and highlights the variation in the percentage of viral families present. These results can be used as a reference for detecting duck viral pathogens and predicting zoonotic potential.
datePublished:2018-01-24T00:00:00Z
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headline:Metagenomic Analysis of the Jinding Duck Fecal Virome
description:Ducks play an important role in transmitting and maintaining mammalian viruses in nature, and are a reservoir host of many animal viruses. We analyzed the fecal virome of four strains (A, B, C, and D) of ducks living in isolation by using metagenomic analysis. The feces of the ducks tested contained 18 animal virus families. The percentage values of RNA virus reads, compared to the total animal virus reads in each of the four strains were 96.96% (A), 97.30% (B), 98.01 (C), and 67.49% (D), and were mainly from Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Reoviridae. Meanwhile, the minority of DNA virus reads were related to Herpesviridae, Adenoviridae, Iridoviridae, and other, low abundance viral families. The percentage values of Orthomyxoviridae, Mimiviridae, Bunyaviridae, Picobirnaviridae, and Herpesviridae reads were not significantly different among strains A, B, and C; however, there were marked differences in the abundance of these reads in strain D. In summary, this study provides an unbiased examination of the viral diversity in the feces of four strains of ducks in specific-pathogen-free periods, and highlights the variation in the percentage of viral families present. These results can be used as a reference for detecting duck viral pathogens and predicting zoonotic potential.
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