We are analyzing https://link.springer.com/article/10.1007/s00262-008-0593-3.

Title:
Characterization of MHC class-I restricted TCRαβ+ CD4− CD8− double negative T cells recognizing the gp100 antigen from a melanoma patient after gp100 vaccination | Cancer Immunology, Immunotherapy
Description:
The immune attack against malignant tumors require the concerted action of CD8+ cytotoxic T lymphocytes (CTL) as well as CD4+ T helper cells. The contribution of T cell receptor (TCR) αβ+ CD4− CD8− double-negative (DN) T cells to anti-tumor immune responses is widely unknown. In previous studies, we have demonstrated that DN T cells with a broad TCR repertoire are present in humans in the peripheral blood and the lymph nodes of healthy individuals. Here, we characterize a human DN T cell clone (T4H2) recognizing an HLA-A2-restricted melanoma-associated antigenic gp100-peptide isolated from the peripheral blood of a melanoma patient. Antigen recognition by the T4H2 DN clone resulted in specific secretion of IFN-γ and TNF. Although lacking the CD8 molecule the gp100-specifc DN T cell clone was able to confer antigen-specific cytotoxicity against gp100-loaded target cells as well as HLA-A2+ gp100 expressing melanoma cells. The cytotoxic capacity was found to be perforin/granzymeB-dependent. Together, these data indicate that functionally active antigen-specific DN T cells recognizing MHC class I-restricted tumor-associated antigen (TAA) may contribute to anti-tumor immunity in vivo.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,734,772 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

While profit motivates many websites, others exist to inspire, entertain, or provide valuable resources. Websites have a variety of goals. And this might be one of them. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

google, scholar, cells, pubmed, cas, article, immunol, cell, cancer, melanoma, antigen, receptor, tcell, mhc, nishimura, mackensen, human, zhang, tcr, doublenegative, recognition, class, res, van, med, moore, exp, privacy, cookies, content, recognizing, patient, voelkl, michael, fischer, lymphocytes, access, specificity, mice, nature, data, information, publish, search, characterization, negative, rehli, andreas, cytotoxic, antitumor,

Topics {✒️}

functionally active melan-a-specific adoptively transferred melan-a-specific fas–fas ligand-independent pathway cd2–cd4–cd8- lymph node month download article/chapter t-cell receptor-transduced cells mrl lpr/lpr mice hla-a2-restricted melanoma cells bearing alpha/beta tumor antigen-reactive tcr human tumor-reactive cd4+ t-cell repertoire diversity confer antigen-specific cytotoxicity single t-cell receptor anti-tumor immune responses human t-cell activation antigenic gp100-peptide isolated progressive immunoproteasome-deficient melanoma cells t-cell receptor cell antigen receptors cd8-dependent antigen recognition gp100-loaded target cells cell cytolytic activity van loenen mm autoimmune gld mice full article pdf article cancer immunology human regressive melanoma peripheral cd4–cd8 broad tcr repertoire privacy choices/manage cookies virus-specific cd8+ cd4+ melanoma-reactive anti-tumor immunity tumor cell recognition” 1-specific tcr related subjects cell receptor binding mart-1 melanoma antigen cell receptor repertoire antitumor activity mediated target cell recognition fischer contributed equally perforin/granzymeb-dependent perforin/granzyme-dependent cd8 enhances formation article voelkl clonally related cells cd4-positive european economic area

Questions {❓}

Van Berkel ME, Oosterwegel MA (2006) CD28 and ICOS: similar or separate costimulators of T cells?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Characterization of MHC class-I restricted TCRαβ+ CD4− CD8− double negative T cells recognizing the gp100 antigen from a melanoma patient after gp100 vaccination
         description:The immune attack against malignant tumors require the concerted action of CD8+ cytotoxic T lymphocytes (CTL) as well as CD4+ T helper cells. The contribution of T cell receptor (TCR) αβ+ CD4− CD8− double-negative (DN) T cells to anti-tumor immune responses is widely unknown. In previous studies, we have demonstrated that DN T cells with a broad TCR repertoire are present in humans in the peripheral blood and the lymph nodes of healthy individuals. Here, we characterize a human DN T cell clone (T4H2) recognizing an HLA-A2-restricted melanoma-associated antigenic gp100-peptide isolated from the peripheral blood of a melanoma patient. Antigen recognition by the T4H2 DN clone resulted in specific secretion of IFN-γ and TNF. Although lacking the CD8 molecule the gp100-specifc DN T cell clone was able to confer antigen-specific cytotoxicity against gp100-loaded target cells as well as HLA-A2+ gp100 expressing melanoma cells. The cytotoxic capacity was found to be perforin/granzymeB-dependent. Together, these data indicate that functionally active antigen-specific DN T cells recognizing MHC class I-restricted tumor-associated antigen (TAA) may contribute to anti-tumor immunity in vivo.
         datePublished:2008-10-03T00:00:00Z
         dateModified:2008-10-03T00:00:00Z
         pageStart:709
         pageEnd:718
         sameAs:https://doi.org/10.1007/s00262-008-0593-3
         keywords:
            Tumor-specific T cells
            DN T cells
            Cancer vaccination
            Tumor-associated antigen
            T-cell receptor
            Melanoma
            Oncology
            Immunology
            Cancer Research
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig6_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig7_HTML.gif
         isPartOf:
            name:Cancer Immunology, Immunotherapy
            issn:
               1432-0851
               0340-7004
            volumeNumber:58
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Simon Voelkl
               affiliation:
                     name:University of Erlangen-Nuernberg
                     address:
                        name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
                        type:PostalAddress
                     type:Organization
                     name:University of Regensburg
                     address:
                        name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tamson V. Moore
               affiliation:
                     name:The University of Chicago
                     address:
                        name:Department of Surgery, The University of Chicago, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Michael Rehli
               affiliation:
                     name:University of Regensburg
                     address:
                        name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Michael I. Nishimura
               affiliation:
                     name:The University of Chicago
                     address:
                        name:Department of Surgery, The University of Chicago, Chicago, USA
                        type:PostalAddress
                     type:Organization
                     name:Medical University of South Carolina
                     address:
                        name:Departments of Surgery and Microbiology and Immunology, Medical University of South Carolina, Charleston, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Andreas Mackensen
               affiliation:
                     name:University of Erlangen-Nuernberg
                     address:
                        name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
                        type:PostalAddress
                     type:Organization
                     name:University of Regensburg
                     address:
                        name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Karin Fischer
               affiliation:
                     name:University of Regensburg
                     address:
                        name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Characterization of MHC class-I restricted TCRαβ+ CD4− CD8− double negative T cells recognizing the gp100 antigen from a melanoma patient after gp100 vaccination
      description:The immune attack against malignant tumors require the concerted action of CD8+ cytotoxic T lymphocytes (CTL) as well as CD4+ T helper cells. The contribution of T cell receptor (TCR) αβ+ CD4− CD8− double-negative (DN) T cells to anti-tumor immune responses is widely unknown. In previous studies, we have demonstrated that DN T cells with a broad TCR repertoire are present in humans in the peripheral blood and the lymph nodes of healthy individuals. Here, we characterize a human DN T cell clone (T4H2) recognizing an HLA-A2-restricted melanoma-associated antigenic gp100-peptide isolated from the peripheral blood of a melanoma patient. Antigen recognition by the T4H2 DN clone resulted in specific secretion of IFN-γ and TNF. Although lacking the CD8 molecule the gp100-specifc DN T cell clone was able to confer antigen-specific cytotoxicity against gp100-loaded target cells as well as HLA-A2+ gp100 expressing melanoma cells. The cytotoxic capacity was found to be perforin/granzymeB-dependent. Together, these data indicate that functionally active antigen-specific DN T cells recognizing MHC class I-restricted tumor-associated antigen (TAA) may contribute to anti-tumor immunity in vivo.
      datePublished:2008-10-03T00:00:00Z
      dateModified:2008-10-03T00:00:00Z
      pageStart:709
      pageEnd:718
      sameAs:https://doi.org/10.1007/s00262-008-0593-3
      keywords:
         Tumor-specific T cells
         DN T cells
         Cancer vaccination
         Tumor-associated antigen
         T-cell receptor
         Melanoma
         Oncology
         Immunology
         Cancer Research
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig6_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00262-008-0593-3/MediaObjects/262_2008_593_Fig7_HTML.gif
      isPartOf:
         name:Cancer Immunology, Immunotherapy
         issn:
            1432-0851
            0340-7004
         volumeNumber:58
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Simon Voelkl
            affiliation:
                  name:University of Erlangen-Nuernberg
                  address:
                     name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
                     type:PostalAddress
                  type:Organization
                  name:University of Regensburg
                  address:
                     name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tamson V. Moore
            affiliation:
                  name:The University of Chicago
                  address:
                     name:Department of Surgery, The University of Chicago, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michael Rehli
            affiliation:
                  name:University of Regensburg
                  address:
                     name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michael I. Nishimura
            affiliation:
                  name:The University of Chicago
                  address:
                     name:Department of Surgery, The University of Chicago, Chicago, USA
                     type:PostalAddress
                  type:Organization
                  name:Medical University of South Carolina
                  address:
                     name:Departments of Surgery and Microbiology and Immunology, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Andreas Mackensen
            affiliation:
                  name:University of Erlangen-Nuernberg
                  address:
                     name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
                     type:PostalAddress
                  type:Organization
                  name:University of Regensburg
                  address:
                     name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Karin Fischer
            affiliation:
                  name:University of Regensburg
                  address:
                     name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cancer Immunology, Immunotherapy
      issn:
         1432-0851
         0340-7004
      volumeNumber:58
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Erlangen-Nuernberg
      address:
         name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
         type:PostalAddress
      name:University of Regensburg
      address:
         name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
         type:PostalAddress
      name:The University of Chicago
      address:
         name:Department of Surgery, The University of Chicago, Chicago, USA
         type:PostalAddress
      name:University of Regensburg
      address:
         name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
         type:PostalAddress
      name:The University of Chicago
      address:
         name:Department of Surgery, The University of Chicago, Chicago, USA
         type:PostalAddress
      name:Medical University of South Carolina
      address:
         name:Departments of Surgery and Microbiology and Immunology, Medical University of South Carolina, Charleston, USA
         type:PostalAddress
      name:University of Erlangen-Nuernberg
      address:
         name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
         type:PostalAddress
      name:University of Regensburg
      address:
         name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
         type:PostalAddress
      name:University of Regensburg
      address:
         name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Simon Voelkl
      affiliation:
            name:University of Erlangen-Nuernberg
            address:
               name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
               type:PostalAddress
            type:Organization
            name:University of Regensburg
            address:
               name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
               type:PostalAddress
            type:Organization
      name:Tamson V. Moore
      affiliation:
            name:The University of Chicago
            address:
               name:Department of Surgery, The University of Chicago, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Michael Rehli
      affiliation:
            name:University of Regensburg
            address:
               name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
               type:PostalAddress
            type:Organization
      name:Michael I. Nishimura
      affiliation:
            name:The University of Chicago
            address:
               name:Department of Surgery, The University of Chicago, Chicago, USA
               type:PostalAddress
            type:Organization
            name:Medical University of South Carolina
            address:
               name:Departments of Surgery and Microbiology and Immunology, Medical University of South Carolina, Charleston, USA
               type:PostalAddress
            type:Organization
      name:Andreas Mackensen
      affiliation:
            name:University of Erlangen-Nuernberg
            address:
               name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
               type:PostalAddress
            type:Organization
            name:University of Regensburg
            address:
               name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Karin Fischer
      affiliation:
            name:University of Regensburg
            address:
               name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
      name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
      name:Department of Surgery, The University of Chicago, Chicago, USA
      name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
      name:Department of Surgery, The University of Chicago, Chicago, USA
      name:Departments of Surgery and Microbiology and Immunology, Medical University of South Carolina, Charleston, USA
      name:Department of Internal Medicine 5, Hematology/Oncology, University of Erlangen-Nuernberg, Erlangen, Germany
      name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
      name:Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(150)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.54s.

LINK . SPRINGER . COM {}