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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
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We are analyzing https://link.springer.com/article/10.1007/s00262-005-0670-9.

Title:
Immune responses to p53 in patients with cancer:enrichment in tetramer+ p53 peptide-specific T cells and regulatory T cells at tumor sites | Cancer Immunology, Immunotherapy
Description:
Objective: A majority of human cancers, including head and neck cancer (HNC), “overexpress” p53. Although T cells specific for wild-type (wt) sequence p53 peptides are detectable in the peripheral blood of patients with HNC, it is unknown whether such T cells accumulate in tumor-involved tissues. Also, the localization of “regulatory” T cells (Treg) to tumor sites in HNC has not been investigated to date. Methods: Tumor infiltrating lymphocytes (TIL), tumor-involved or non-involved lymph node lymphocytes (LNL) and peripheral blood mononuclear cells (PBMC) were obtained from 24 HLA-A2.1+ patients with HNC. Using tetramers and four-color flow cytometry, the frequency of Treg and CD3+CD8+ T cells specific for wt p53 epitopes as well as their functional attributes were determined. Results: The CD3+CD8+ tetramer+ cell frequency was significantly higher (P<0.001) in TIL than autologous PBMC as was the percentage of CD4+CD25+ T cells (P<0.003). TIL were enriched in FOXp3+, GITR+ and CTLA-4+ Treg. CD8+ TIL had low Ζ expression and produced little IFN-γ after ex vivo stimulation relative to autologous PBMC or PBMC from NC. Conclusions: Anti-wt p53 epitope-specific T cells and Treg preferentially localize to tumor sites in patients with HNC. However, despite enrichment in tumor peptide-specific T cells, the effector cell population (CD3+CD8+) in TIL or PBMC was unresponsive to activation in the tumor microenvironment enriched in Treg.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Science
  • Health & Fitness
  • Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

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How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

cells, cancer, google, scholar, article, pubmed, cas, whiteside, patients, regulatory, tumor, cdcd, immunol, human, head, neck, cell, peripheral, blood, res, deleo, specific, wildtype, treg, lymphocytes, til, access, tumors, pittsburgh, privacy, cookies, content, research, immunology, immune, hnc, pbmc, clin, donnenberg, publish, search, tetramer, sites, albers, kim, theresa, immunity, peptide, data, information,

Topics {✒️}

p53 wild-type epitope-specific cd8+cd28- t-cell subset tumor-suppressor gene-product p53 anti-wt p53 epitope-specific month download article/chapter nih grants po1-de12321 tumor-involved lymph nodes late-stage ovarian cancer wt p53 epitopes tetramer+ p53 peptide-specific article cancer immunology full article pdf anti-p53 cytotoxic human cd4+cd25+ cells privacy choices/manage cookies sequence p53 peptides neck cancer research epitope-loss variants tnf receptor superfamily stout family fund related subjects removing cd25+cd4+ tumor escape access tumor peptide-specific tumor-induced death peptide-mhc class human cd4+cd25+ tumor-involved tissues low ζ expression clinical laboratory immunology cd4+cd25+ regulatory regulatory cd4+cd25+ lymphoid suppressor cells european economic area wild-type vivo stimulation relative naturally occurring population ligand mediates resistance hillman cancer center t-cell recognition specific immune response tumor infiltrating lymphocytes tumor-infiltrating lymphocytes conditions privacy policy somatic point mutations oral oncol 32b effector cell population accepting optional cookies color flow cytometry predicts reduced survival

Questions {❓}

  • Ramsdell F (2003) Foxp3 and natural regulatory T cells: key to a cell lineage?

Schema {🗺️}

WebPage:
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         headline:Immune responses to p53 in patients with cancer:enrichment in tetramer+ p53 peptide-specific T cells and regulatory T cells at tumor sites
         description:Objective: A majority of human cancers, including head and neck cancer (HNC), “overexpress” p53. Although T cells specific for wild-type (wt) sequence p53 peptides are detectable in the peripheral blood of patients with HNC, it is unknown whether such T cells accumulate in tumor-involved tissues. Also, the localization of “regulatory” T cells (Treg) to tumor sites in HNC has not been investigated to date. Methods: Tumor infiltrating lymphocytes (TIL), tumor-involved or non-involved lymph node lymphocytes (LNL) and peripheral blood mononuclear cells (PBMC) were obtained from 24 HLA-A2.1+ patients with HNC. Using tetramers and four-color flow cytometry, the frequency of Treg and CD3+CD8+ T cells specific for wt p53 epitopes as well as their functional attributes were determined. Results: The CD3+CD8+ tetramer+ cell frequency was significantly higher (P<0.001) in TIL than autologous PBMC as was the percentage of CD4+CD25+ T cells (P<0.003). TIL were enriched in FOXp3+, GITR+ and CTLA-4+ Treg. CD8+ TIL had low Ζ expression and produced little IFN-γ after ex vivo stimulation relative to autologous PBMC or PBMC from NC. Conclusions: Anti-wt p53 epitope-specific T cells and Treg preferentially localize to tumor sites in patients with HNC. However, despite enrichment in tumor peptide-specific T cells, the effector cell population (CD3+CD8+) in TIL or PBMC was unresponsive to activation in the tumor microenvironment enriched in Treg.
         datePublished:2005-06-16T00:00:00Z
         dateModified:2005-06-16T00:00:00Z
         pageStart:1072
         pageEnd:1081
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            Regulatory T cells (Treg)
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            Immunology
            Cancer Research
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      headline:Immune responses to p53 in patients with cancer:enrichment in tetramer+ p53 peptide-specific T cells and regulatory T cells at tumor sites
      description:Objective: A majority of human cancers, including head and neck cancer (HNC), “overexpress” p53. Although T cells specific for wild-type (wt) sequence p53 peptides are detectable in the peripheral blood of patients with HNC, it is unknown whether such T cells accumulate in tumor-involved tissues. Also, the localization of “regulatory” T cells (Treg) to tumor sites in HNC has not been investigated to date. Methods: Tumor infiltrating lymphocytes (TIL), tumor-involved or non-involved lymph node lymphocytes (LNL) and peripheral blood mononuclear cells (PBMC) were obtained from 24 HLA-A2.1+ patients with HNC. Using tetramers and four-color flow cytometry, the frequency of Treg and CD3+CD8+ T cells specific for wt p53 epitopes as well as their functional attributes were determined. Results: The CD3+CD8+ tetramer+ cell frequency was significantly higher (P<0.001) in TIL than autologous PBMC as was the percentage of CD4+CD25+ T cells (P<0.003). TIL were enriched in FOXp3+, GITR+ and CTLA-4+ Treg. CD8+ TIL had low Ζ expression and produced little IFN-γ after ex vivo stimulation relative to autologous PBMC or PBMC from NC. Conclusions: Anti-wt p53 epitope-specific T cells and Treg preferentially localize to tumor sites in patients with HNC. However, despite enrichment in tumor peptide-specific T cells, the effector cell population (CD3+CD8+) in TIL or PBMC was unresponsive to activation in the tumor microenvironment enriched in Treg.
      datePublished:2005-06-16T00:00:00Z
      dateModified:2005-06-16T00:00:00Z
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      pageEnd:1081
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         TIL
         Tetramers
         wt p53 epitopes
         CD4+CD25+ T cells
         Regulatory T cells (Treg)
         Tumor escape
         Oncology
         Immunology
         Cancer Research
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                     name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
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                     type:PostalAddress
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               type:PostalAddress
            type:Organization
      name:Theresa L. Whiteside
      affiliation:
            name:University of Pittsburgh School of Medicine
            address:
               name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
               type:PostalAddress
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      email:[email protected]
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      name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
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      name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
      name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
      name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
      name:University of Pittsburgh Cancer Institute and Departments of Pathology (ABD, TLW), Immunology and Otolaryngology (RLF, TLW), University of Pittsburgh School of Medicine, Pittsburgh, USA
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External Links {🔗}(105)

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