We are analyzing https://link.springer.com/article/10.1007/s00262-002-0273-7.

Title:
A simple culture protocol to detect peptide-specific cytotoxic T lymphocyte precursors in the circulation | Cancer Immunology, Immunotherapy
Description:
The detection and monitoring of peptide-specific cytotoxic T lymphocyte (CTL) precursors is essential for successful peptide-based immunotherapy against cancers. In contrast to the development of effective methods of detecting antigen-specific CTL, such as ELISpot and HLA-class I tetramer assay, stimulation with peptide-pulsed antigen-presenting cells (APC) has for some time been conventionally employed to induce peptide-specific CTL from peripheral blood mononuclear cells (PBMC). This culture protocol, however, needs a substantial number of PBMC to test the reactivity against a panel of peptides. In the present study, we established a simple culture protocol which has no need of additional APC. Addition of a corresponding peptide every 3 days was found to induce not only Epstein–Barr virus (EBV)-specific CTL from healthy donors, but also tumor antigen-derived peptide-specific CTL from cancer patients. A 10-ml blood sample was almost sufficient to test the presence of CTL precursors against 20 different peptides in triplicate assays. Overall, this culture protocol can be useful in detecting and monitoring peptide-specific CTL precursors in the circulation in peptide-based immunotherapy against cancer.
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Keywords {🔍}

article, ctl, culture, peptidespecific, privacy, cookies, content, cancer, immunotherapy, protocol, cytotoxic, precursors, peptide, access, information, publish, search, lymphocyte, data, log, journal, research, immunology, simple, circulation, hida, maeda, katagiri, cells, discover, japan, springer, essential, optional, personal, parties, policy, find, track, detect, june, cite, naoya, yoshiaki, kazuko, hideo, takasu, mamoru, harada, kyogo,

Topics {✒️}

peptide-pulsed antigen-presenting cells mamoru harada & kyogo itoh detect peptide-specific cytotoxic successful peptide-based immunotherapy month download article/chapter induce peptide-specific ctl detecting antigen-specific ctl peptide-specific cytotoxic peptide-based immunotherapy privacy choices/manage cookies related subjects full article pdf article cancer immunology simple culture protocol 10-ml blood sample 0201-restricted ctl epitope european economic area hideo takasu epstein–barr virus kurume university school conditions privacy policy accepting optional cookies june 2002 volume 51 specific ctl 0201-restricted cytotoxic journal finder publish immunotherapy article check access instant access article hida culture protocol naoya hida article log yoshiaki maeda ctl precursors additional apc peptide privacy policy personal data books a immunotherapy aims article cite optional cookies manage preferences cancer patients subscription content similar content data protection cookies skip institution subscribe

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         headline:A simple culture protocol to detect peptide-specific cytotoxic T lymphocyte precursors in the circulation
         description: The detection and monitoring of peptide-specific cytotoxic T lymphocyte (CTL) precursors is essential for successful peptide-based immunotherapy against cancers. In contrast to the development of effective methods of detecting antigen-specific CTL, such as ELISpot and HLA-class I tetramer assay, stimulation with peptide-pulsed antigen-presenting cells (APC) has for some time been conventionally employed to induce peptide-specific CTL from peripheral blood mononuclear cells (PBMC). This culture protocol, however, needs a substantial number of PBMC to test the reactivity against a panel of peptides. In the present study, we established a simple culture protocol which has no need of additional APC. Addition of a corresponding peptide every 3 days was found to induce not only Epstein–Barr virus (EBV)-specific CTL from healthy donors, but also tumor antigen-derived peptide-specific CTL from cancer patients. A 10-ml blood sample was almost sufficient to test the presence of CTL precursors against 20 different peptides in triplicate assays. Overall, this culture protocol can be useful in detecting and monitoring peptide-specific CTL precursors in the circulation in peptide-based immunotherapy against cancer.
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      headline:A simple culture protocol to detect peptide-specific cytotoxic T lymphocyte precursors in the circulation
      description: The detection and monitoring of peptide-specific cytotoxic T lymphocyte (CTL) precursors is essential for successful peptide-based immunotherapy against cancers. In contrast to the development of effective methods of detecting antigen-specific CTL, such as ELISpot and HLA-class I tetramer assay, stimulation with peptide-pulsed antigen-presenting cells (APC) has for some time been conventionally employed to induce peptide-specific CTL from peripheral blood mononuclear cells (PBMC). This culture protocol, however, needs a substantial number of PBMC to test the reactivity against a panel of peptides. In the present study, we established a simple culture protocol which has no need of additional APC. Addition of a corresponding peptide every 3 days was found to induce not only Epstein–Barr virus (EBV)-specific CTL from healthy donors, but also tumor antigen-derived peptide-specific CTL from cancer patients. A 10-ml blood sample was almost sufficient to test the presence of CTL precursors against 20 different peptides in triplicate assays. Overall, this culture protocol can be useful in detecting and monitoring peptide-specific CTL precursors in the circulation in peptide-based immunotherapy against cancer.
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