We are analyzing https://link.springer.com/article/10.1007/s00216-009-2895-5.

Title:
An SEC/MALS study of alternan degradation during size-exclusion chromatographic analysis | Analytical and Bioanalytical Chemistry
Description:
Ultrahigh-molar-mass (M) polymers such as DNA, cellulose, and polyolefins are routinely analyzed using size-exclusion chromatography (SEC) to obtain molar mass averages, distributions, and architectural information. It has long been contended that high-M polymers can degrade during SEC analysis; if true, the inaccurate molar mass information obtained can adversely affect decisions regarding processing and end-use properties of the macromolecules. However, most evidence to the effect of degradation has been circumstantial and open to alternative interpretation. For example, the shift in SEC elution volume as a function of increased chromatographic flow rate, observed using only a concentration-sensitive detector, may be the result of degradation or of elution via a nondegradatory slalom chromatography mechanism. Here, using both concentration-sensitive and multiangle static light-scattering detection, we provide unambiguous evidence that the polysaccharide alternan actually degrades during SEC analysis. The decrease in molar mass and size of alternan with increasing flow rate, measured using light scattering, allows ruling out an SC mode of elution and can only be interpreted as due to degradation. These findings demonstrate the extreme fragility of ultrahigh-M polymers and the care that must be taken for accurate characterization.
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Topics {✒️}

size-exclusion chromatographic analysis month download article/chapter acs symposium series size-exclusion chromatography size exclusion chromatography increasing flow rate full article pdf molar mass distribution privacy choices/manage cookies biocatalysis research unit analytical ultracentrifuge determination light scattering van den hoed florida state university related materials iii acknowledge amandaa brewer article analytical european economic area adversely affect decisions american chemical society imad haidar ahmad conditions privacy policy carlin fj jr bioanalytical chemistry aims molar mass sec/mals study accepting optional cookies concentration-sensitive detector provide unambiguous evidence usda ars ncaur related subjects côté gl article striegel main content log author information authors journal finder publish liquid chromatography kirkland jj article log check access instant access article cite sec elution volume mukherji ak privacy policy sec-mals anal chem 77 liq chromatogr 7 liq chromatogr 5 sec analysis

Schema {🗺️}

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         headline:An SEC/MALS study of alternan degradation during size-exclusion chromatographic analysis
         description:Ultrahigh-molar-mass (M) polymers such as DNA, cellulose, and polyolefins are routinely analyzed using size-exclusion chromatography (SEC) to obtain molar mass averages, distributions, and architectural information. It has long been contended that high-M polymers can degrade during SEC analysis; if true, the inaccurate molar mass information obtained can adversely affect decisions regarding processing and end-use properties of the macromolecules. However, most evidence to the effect of degradation has been circumstantial and open to alternative interpretation. For example, the shift in SEC elution volume as a function of increased chromatographic flow rate, observed using only a concentration-sensitive detector, may be the result of degradation or of elution via a nondegradatory slalom chromatography mechanism. Here, using both concentration-sensitive and multiangle static light-scattering detection, we provide unambiguous evidence that the polysaccharide alternan actually degrades during SEC analysis. The decrease in molar mass and size of alternan with increasing flow rate, measured using light scattering, allows ruling out an SC mode of elution and can only be interpreted as due to degradation. These findings demonstrate the extreme fragility of ultrahigh-M polymers and the care that must be taken for accurate characterization.
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            Size-exclusion chromatography
            On-column flow-induced degradation
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            Analytical Chemistry
            Biochemistry
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            Characterization and Evaluation of Materials
            Food Science
            Monitoring/Environmental Analysis
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      headline:An SEC/MALS study of alternan degradation during size-exclusion chromatographic analysis
      description:Ultrahigh-molar-mass (M) polymers such as DNA, cellulose, and polyolefins are routinely analyzed using size-exclusion chromatography (SEC) to obtain molar mass averages, distributions, and architectural information. It has long been contended that high-M polymers can degrade during SEC analysis; if true, the inaccurate molar mass information obtained can adversely affect decisions regarding processing and end-use properties of the macromolecules. However, most evidence to the effect of degradation has been circumstantial and open to alternative interpretation. For example, the shift in SEC elution volume as a function of increased chromatographic flow rate, observed using only a concentration-sensitive detector, may be the result of degradation or of elution via a nondegradatory slalom chromatography mechanism. Here, using both concentration-sensitive and multiangle static light-scattering detection, we provide unambiguous evidence that the polysaccharide alternan actually degrades during SEC analysis. The decrease in molar mass and size of alternan with increasing flow rate, measured using light scattering, allows ruling out an SC mode of elution and can only be interpreted as due to degradation. These findings demonstrate the extreme fragility of ultrahigh-M polymers and the care that must be taken for accurate characterization.
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         Size-exclusion chromatography
         On-column flow-induced degradation
         Multiangle light scattering
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         Biochemistry
         general
         Laboratory Medicine
         Characterization and Evaluation of Materials
         Food Science
         Monitoring/Environmental Analysis
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