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Title:
Taurine supplementation to a low protein diet during foetal and early postnatal life restores a normal proliferation and apoptosis of rat pancreatic islets | Diabetologia
Description:
Aims/hypothesis. In our previous studies a low protein diet (8% vs 20%) given during foetal and early postnatal life induced abnormal development of the endocrine pancreas; beta-cell mass and islet-cell proliferation were reduced while apoptosis was increased. Taurine, an important amino acid for development was also reduced in maternal and foetal plasma of protein deficient animals. In this study we aim to evaluate the role of taurine in the alterations observed in rats after a low protein diet. Methods. Four groups of rats were given either a control, a low protein, or control and low protein diets with 2.5% taurine in the drinking water. Diets were given to gestating and lactating mothers and to their pups until day 30. Beta and endocrine cell masses were analysed as well as DNA synthesis and apoptosis after taurine supplementation in foetuses and pups. We also investigated insulin like growth factor-II (IGF-II), inducible nitric oxide synthase (iNOS), and Fas by immunohistochemistry. Results. In foetuses and neonates nourished with a low protein diet, taurine supplementation restored normal DNA synthesis and apoptosis. This led to adequate beta and endocrine cell mass in pups. In islet cells, immunoreactivity was increased for IGF-II, reduced for Fas and unchanged for iNOS after taurine supplementation. Conclusion/interpretation. Taurine supplementation to a low protein diet in foetal and early postnatal life prevents the abnormal development of the endocrine pancreas. The mechanisms by which taurine acts on DNA synthesis and apoptosis rate of endocrine cells involve IGF-II, Fas regulation but not iNOS.
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taurine, protein, low, article, supplementation, diet, endocrine, apoptosis, privacy, cookies, foetal, development, content, information, publish, research, search, early, postnatal, life, igfii, fas, data, journal, diabetologia, normal, proliferation, rat, pancreatic, islets, download, boujendar, reusens, merezak, pancreas, reduced, rats, pups, dna, synthesis, inos, discover, health, ontario, function, optional, personal, parties, policy, find,
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rat pancreatic islets low protein diet related subjects privacy choices/manage cookies growth factor-ii endocrine cell masses endocrine cell mass igf-ii low protein diets protein deficient animals dietary protein modulates main content log european economic area important amino acid abnormal development conditions privacy policy ontario n6a 4v2 accepting optional cookies low protein scope submit manuscript beta-cell mass islet-cell proliferation louvain-la-neuve search search adaptive islet function journal finder publish endocrine pancreas taurine supplementation dna synthesis article cite fas regulation article boujendar islet cells privacy policy normal proliferation personal data health care books a taurine acts rats optional cookies information manage preferences development diabetologia 45 data protection essential cookies cookies skip maternal fas
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mainEntity:
headline:Taurine supplementation to a low protein diet during foetal and early postnatal life restores a normal proliferation and apoptosis of rat pancreatic islets
description:
Aims/hypothesis. In our previous studies a low protein diet (8% vs 20%) given during foetal and early postnatal life induced abnormal development of the endocrine pancreas; beta-cell mass and islet-cell proliferation were reduced while apoptosis was increased. Taurine, an important amino acid for development was also reduced in maternal and foetal plasma of protein deficient animals. In this study we aim to evaluate the role of taurine in the alterations observed in rats after a low protein diet.
Methods. Four groups of rats were given either a control, a low protein, or control and low protein diets with 2.5% taurine in the drinking water. Diets were given to gestating and lactating mothers and to their pups until day 30. Beta and endocrine cell masses were analysed as well as DNA synthesis and apoptosis after taurine supplementation in foetuses and pups. We also investigated insulin like growth factor-II (IGF-II), inducible nitric oxide synthase (iNOS), and Fas by immunohistochemistry.
Results. In foetuses and neonates nourished with a low protein diet, taurine supplementation restored normal DNA synthesis and apoptosis. This led to adequate beta and endocrine cell mass in pups. In islet cells, immunoreactivity was increased for IGF-II, reduced for Fas and unchanged for iNOS after taurine supplementation.
Conclusion/interpretation. Taurine supplementation to a low protein diet in foetal and early postnatal life prevents the abnormal development of the endocrine pancreas. The mechanisms by which taurine acts on DNA synthesis and apoptosis rate of endocrine cells involve IGF-II, Fas regulation but not iNOS.
datePublished:2002-05-08T00:00:00Z
dateModified:2002-05-08T00:00:00Z
pageStart:856
pageEnd:866
sameAs:https://doi.org/10.1007/s00125-002-0833-6
keywords:
Rats development low protein diet taurine pancreatic islets BrdU TUNEL IGF-II Fas
Internal Medicine
Metabolic Diseases
Human Physiology
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1432-0428
0012-186X
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name:S. Boujendar
affiliation:
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ScholarlyArticle:
headline:Taurine supplementation to a low protein diet during foetal and early postnatal life restores a normal proliferation and apoptosis of rat pancreatic islets
description:
Aims/hypothesis. In our previous studies a low protein diet (8% vs 20%) given during foetal and early postnatal life induced abnormal development of the endocrine pancreas; beta-cell mass and islet-cell proliferation were reduced while apoptosis was increased. Taurine, an important amino acid for development was also reduced in maternal and foetal plasma of protein deficient animals. In this study we aim to evaluate the role of taurine in the alterations observed in rats after a low protein diet.
Methods. Four groups of rats were given either a control, a low protein, or control and low protein diets with 2.5% taurine in the drinking water. Diets were given to gestating and lactating mothers and to their pups until day 30. Beta and endocrine cell masses were analysed as well as DNA synthesis and apoptosis after taurine supplementation in foetuses and pups. We also investigated insulin like growth factor-II (IGF-II), inducible nitric oxide synthase (iNOS), and Fas by immunohistochemistry.
Results. In foetuses and neonates nourished with a low protein diet, taurine supplementation restored normal DNA synthesis and apoptosis. This led to adequate beta and endocrine cell mass in pups. In islet cells, immunoreactivity was increased for IGF-II, reduced for Fas and unchanged for iNOS after taurine supplementation.
Conclusion/interpretation. Taurine supplementation to a low protein diet in foetal and early postnatal life prevents the abnormal development of the endocrine pancreas. The mechanisms by which taurine acts on DNA synthesis and apoptosis rate of endocrine cells involve IGF-II, Fas regulation but not iNOS.
datePublished:2002-05-08T00:00:00Z
dateModified:2002-05-08T00:00:00Z
pageStart:856
pageEnd:866
sameAs:https://doi.org/10.1007/s00125-002-0833-6
keywords:
Rats development low protein diet taurine pancreatic islets BrdU TUNEL IGF-II Fas
Internal Medicine
Metabolic Diseases
Human Physiology
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1432-0428
0012-186X
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name:Laboratory of Cellular Biology, World Health Collaborating Center for the Development of the Endocrine Pancreas, Catholic University of Louvain, Louvain-la-Neuve, Belgium
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name:Lawson Health Research Institute, St. Joseph's Health Care, London Ontario; and Departments of Medicine, Physiology and Paediatrics, University of Western Ontario, Ontario N6A 4V2, Canada
name:Lawson Health Research Institute, St. Joseph's Health Care, London Ontario; and Departments of Medicine, Physiology and Paediatrics, University of Western Ontario, Ontario N6A 4V2, Canada
name:Laboratory of Cellular Biology, World Health Collaborating Center for the Development of the Endocrine Pancreas, Catholic University of Louvain, Louvain-la-Neuve, Belgium
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