We are analyzing https://link.springer.com/article/10.1007/s00122-005-0062-8.

Title:
Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests | Theoretical and Applied Genetics
Description:
A novel synthetic cry2A* gene was introduced into the elite indica rice restorer line Minghui 63 by Agrobacterium-mediated transformation. A total of 102 independent transformants were obtained. Among them, 71 transformants were positive cry2A* plants according to PCR analysis. Four highly insect-resistant lines with single-copy insertion (designated as 2A-1, 2A-2, 2A-3, and 2A-4) were selected based on field assessment and Southern blot analysis in the T1 generation. All four transgenic lines showed Mendelian segregation by seed germination on 1/2 MS medium containing Basta. Homozygous transgenic plants were selected according to germination ratio (100%) in the T2 generation. Cry2A* protein concentrations were determined in homozygous transgenic lines, their derived hybrids, and their backcross offspring. The Cry2A* protein concentrations of four homozygous transgenic lines ranged from 9.65 to 12.11 μg/g of leaf fresh weight. There was little variation in the hybrids and backcross offspring. Insect bioassays were conducted in both the laboratory and field. All four transgenic lines were significantly resistant to lepidopteran rice pests. These cry2A* transgenic lines can be used to produce insect-resistant hybrids and serve as a resistant source for the development of two-toxin Bt rice.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Food & Drink

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

article, rice, google, scholar, transgenic, cas, plants, bacillus, thuringiensis, pubmed, gene, resistance, stem, plant, borer, indica, synthetic, crya, lines, insect, resistant, expressing, research, zhang, field, privacy, cookies, content, analysis, data, pests, access, nat, biotechnol, information, publish, search, genetics, lepidopteran, chen, lin, leaf, development, δendotoxin, riazuddin, mol, zhao, roush, shelton, crops,

Topics {✒️}

leaf fresh weight month download article/chapter bacillus thuringiensis δ-endotoxins transgenic plants expressing yongjun lin & qifa zhang transgenic rice expressing yellow stem borer lepidopteran rice pests plant gene research synthetic cry1ab gene applied genetics aims homozygous transgenic plants striped stem borer stripe stem borer bt transgenic plants synthetic cry2a gene highly insect-resistant lines insecticidal cry1ab gene homozygous transgenic lines rice leaf folders rice leaf folder bacillus thuringiensis berliner produce insect-resistant hybrids indica basmati rice cry2a transgenic lines related subjects tissue culture conditions insecticidal transgenic crops huazhong agricultural university crop genetic improvement commercialized biotech/gm crops rice stem borers full article pdf positive cry2a plants transgenic plants transgenic bt crops privacy choices/manage cookies δ-endotoxin agrobacterium-mediated transformation high efficiency transformation toxin bt rice managing insect resistance oregon state university hybrid rice transgenic rice bacillus thuringiensis plant physiol 15 transgenic lines receptor binding properties insect-resistance management

Questions {❓}

Roush RT (1998) Two-toxin strategies for management of insecticidal transgenic crops: can pyramiding succeed where pesticide mixtures have not?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests
         description:A novel synthetic cry2A* gene was introduced into the elite indica rice restorer line Minghui 63 by Agrobacterium-mediated transformation. A total of 102 independent transformants were obtained. Among them, 71 transformants were positive cry2A* plants according to PCR analysis. Four highly insect-resistant lines with single-copy insertion (designated as 2A-1, 2A-2, 2A-3, and 2A-4) were selected based on field assessment and Southern blot analysis in the T1 generation. All four transgenic lines showed Mendelian segregation by seed germination on 1/2 MS medium containing Basta. Homozygous transgenic plants were selected according to germination ratio (100%) in the T2 generation. Cry2A* protein concentrations were determined in homozygous transgenic lines, their derived hybrids, and their backcross offspring. The Cry2A* protein concentrations of four homozygous transgenic lines ranged from 9.65 to 12.11 μg/g of leaf fresh weight. There was little variation in the hybrids and backcross offspring. Insect bioassays were conducted in both the laboratory and field. All four transgenic lines were significantly resistant to lepidopteran rice pests. These cry2A* transgenic lines can be used to produce insect-resistant hybrids and serve as a resistant source for the development of two-toxin Bt rice.
         datePublished:2005-09-27T00:00:00Z
         dateModified:2005-09-27T00:00:00Z
         pageStart:1330
         pageEnd:1337
         sameAs:https://doi.org/10.1007/s00122-005-0062-8
         keywords:
            Transgenic Plant
            Transgenic Line
            Stem Borer
            Leaf Fresh Weight
            Yellow Stem Borer
            Plant Breeding/Biotechnology
            Plant Genetics and Genomics
            Agriculture
            Plant Biochemistry
            Biochemistry
            general
            Biotechnology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig5_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig6_HTML.jpg
         isPartOf:
            name:Theoretical and Applied Genetics
            issn:
               1432-2242
               0040-5752
            volumeNumber:111
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Hao Chen
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Wei Tang
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Caiguo Xu
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xianghua Li
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yongjun Lin
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Qifa Zhang
               affiliation:
                     name:Huazhong Agricultural University
                     address:
                        name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests
      description:A novel synthetic cry2A* gene was introduced into the elite indica rice restorer line Minghui 63 by Agrobacterium-mediated transformation. A total of 102 independent transformants were obtained. Among them, 71 transformants were positive cry2A* plants according to PCR analysis. Four highly insect-resistant lines with single-copy insertion (designated as 2A-1, 2A-2, 2A-3, and 2A-4) were selected based on field assessment and Southern blot analysis in the T1 generation. All four transgenic lines showed Mendelian segregation by seed germination on 1/2 MS medium containing Basta. Homozygous transgenic plants were selected according to germination ratio (100%) in the T2 generation. Cry2A* protein concentrations were determined in homozygous transgenic lines, their derived hybrids, and their backcross offspring. The Cry2A* protein concentrations of four homozygous transgenic lines ranged from 9.65 to 12.11 μg/g of leaf fresh weight. There was little variation in the hybrids and backcross offspring. Insect bioassays were conducted in both the laboratory and field. All four transgenic lines were significantly resistant to lepidopteran rice pests. These cry2A* transgenic lines can be used to produce insect-resistant hybrids and serve as a resistant source for the development of two-toxin Bt rice.
      datePublished:2005-09-27T00:00:00Z
      dateModified:2005-09-27T00:00:00Z
      pageStart:1330
      pageEnd:1337
      sameAs:https://doi.org/10.1007/s00122-005-0062-8
      keywords:
         Transgenic Plant
         Transgenic Line
         Stem Borer
         Leaf Fresh Weight
         Yellow Stem Borer
         Plant Breeding/Biotechnology
         Plant Genetics and Genomics
         Agriculture
         Plant Biochemistry
         Biochemistry
         general
         Biotechnology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig5_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00122-005-0062-8/MediaObjects/122_2005_62_Fig6_HTML.jpg
      isPartOf:
         name:Theoretical and Applied Genetics
         issn:
            1432-2242
            0040-5752
         volumeNumber:111
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Hao Chen
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wei Tang
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Caiguo Xu
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xianghua Li
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yongjun Lin
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Qifa Zhang
            affiliation:
                  name:Huazhong Agricultural University
                  address:
                     name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Theoretical and Applied Genetics
      issn:
         1432-2242
         0040-5752
      volumeNumber:111
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
      name:Huazhong Agricultural University
      address:
         name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Hao Chen
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Wei Tang
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Caiguo Xu
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Xianghua Li
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Yongjun Lin
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Qifa Zhang
      affiliation:
            name:Huazhong Agricultural University
            address:
               name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
               type:PostalAddress
            type:Organization
PostalAddress:
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People’s Republic of China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(102)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

4.26s.

LINK . SPRINGER . COM {}