We are analyzing https://link.springer.com/article/10.1007/bf02623578.

Title:
A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium | In Vitro Cellular & Developmental Biology - Plant
Description:
A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrene antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating “stem cell”-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of brest cell differentiation and carcinogenesis in vitro.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

google, scholar, cas, cell, human, pubmed, article, breast, cells, mammary, epithelial, cancer, growth, vitro, line, res, culture, normal, defined, medium, cultures, differentiation, access, lines, petersen, van, deurs, jnci, sci, proc, usa, privacy, cookies, content, information, nontumorigenic, tissue, nature, milk, estrogen, antibodies, characterization, natl, acad, biol, publish, search, diploid, chemically, briand,

Topics {✒️}

month download article/chapter epithelial-mesenchymal transitions indicating “stem cell” early-lactation human milk serum-free medium serum-free growth explore breast morphogenesis human breast milk mammary epithelial cells human breast cancer human breast tissue epithelial cell replication epithelial cell islets aneuploid mammary epithelial human breast tissues human mammary gland d492 cell lines syngeneic cell lines luminal epithelial cells hmt-3522 cell line human breast carcinomas continuous cell lines human mammary epithelium cell line established related subjects full article pdf floating collagen membranes fibrocystic breast tissue chemically defined medium privacy choices/manage cookies normal breast epithelium human tumor cells malignant breast diseases low serum concentrations defines human myoepithelium human lacteal secretions cell tissue res collagen gel culture cell line brest cell differentiation malignant breast tumors polarized cells connected long-term growth check access instant access danish cancer society basement membrane proteins long term cultivation epidermal growth factor european economic area

Schema {🗺️}

WebPage:
      mainEntity:
         headline:A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium
         description:A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrene antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating “stem cell”-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of brest cell differentiation and carcinogenesis in vitro.
         datePublished:
         dateModified:
         pageStart:181
         pageEnd:188
         sameAs:https://doi.org/10.1007/BF02623578
         keywords:
            human breast
            nontumorigenic epithelial cell line
            cell characterization
            serum-free medium
            Plant Sciences
            Cell Biology
            Developmental Biology
            Plant Breeding/Biotechnology
            Plant Genetics and Genomics
         image:
         isPartOf:
            name:In Vitro Cellular & Developmental Biology
            issn:
               1475-2689
               0883-8364
            volumeNumber:23
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:P. Briand
               affiliation:
                     name:The Fibiger Institute
                     address:
                        name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                        type:PostalAddress
                     type:Organization
               type:Person
               name:O. W. Petersen
               affiliation:
                     name:The Fibiger Institute
                     address:
                        name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                        type:PostalAddress
                     type:Organization
               type:Person
               name:B. Van Deurs
               affiliation:
                     name:The Fibiger Institute
                     address:
                        name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                        type:PostalAddress
                     type:Organization
                     name:University of Copenhagen
                     address:
                        name:Department of Anatomoy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium
      description:A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrene antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating “stem cell”-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of brest cell differentiation and carcinogenesis in vitro.
      datePublished:
      dateModified:
      pageStart:181
      pageEnd:188
      sameAs:https://doi.org/10.1007/BF02623578
      keywords:
         human breast
         nontumorigenic epithelial cell line
         cell characterization
         serum-free medium
         Plant Sciences
         Cell Biology
         Developmental Biology
         Plant Breeding/Biotechnology
         Plant Genetics and Genomics
      image:
      isPartOf:
         name:In Vitro Cellular & Developmental Biology
         issn:
            1475-2689
            0883-8364
         volumeNumber:23
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:P. Briand
            affiliation:
                  name:The Fibiger Institute
                  address:
                     name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
            type:Person
            name:O. W. Petersen
            affiliation:
                  name:The Fibiger Institute
                  address:
                     name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
            type:Person
            name:B. Van Deurs
            affiliation:
                  name:The Fibiger Institute
                  address:
                     name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
                  name:University of Copenhagen
                  address:
                     name:Department of Anatomoy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:In Vitro Cellular & Developmental Biology
      issn:
         1475-2689
         0883-8364
      volumeNumber:23
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:The Fibiger Institute
      address:
         name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
         type:PostalAddress
      name:The Fibiger Institute
      address:
         name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
         type:PostalAddress
      name:The Fibiger Institute
      address:
         name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
         type:PostalAddress
      name:University of Copenhagen
      address:
         name:Department of Anatomoy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:P. Briand
      affiliation:
            name:The Fibiger Institute
            address:
               name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
      name:O. W. Petersen
      affiliation:
            name:The Fibiger Institute
            address:
               name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
      name:B. Van Deurs
      affiliation:
            name:The Fibiger Institute
            address:
               name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
            name:University of Copenhagen
            address:
               name:Department of Anatomoy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
      name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
      name:Laboratory of Tumor Endocrinology, The Fibiger Institute, Copenhagen, Denmark
      name:Department of Anatomoy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(141)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.87s.

LINK . SPRINGER . COM {}