We are analyzing https://link.springer.com/article/10.1007/bf01993963.

Title:
Angiotensin II, vasopressin and GTP[γ-S] inhibit inward-rectifying K+ channels in porcine cerebral capillary endothelial cells | The Journal of Membrane Biology
Description:
Cerebral capillaries from porcine brain were isolated. and endothelial cells were grown in primary culture. The whole-cell tight seal patch-clamp method was applied to freshly isolated single endothelial cells, and cells which were held in culture up to one week. With high K+ solution in the patch pipette and in the bath we observed inward-rectifying K+ currents, showing a time-dependent decay in part of the experiments. Ba2+ (1–10mm) in the bath blocked this current, whereas outside tetraethylammonium (10mm) decreased the peak current but increased the steady-state current. Addition of 1 μm of angiotensin II or of arginine-vasopressin to the extracellular side caused a time-dependent inhibition of the inward-rectifying K+ current in part of the experiments. Addition of 100 μm GTP[γ-S] to the patch pipette blocked the K+ inward rectifier. In cell-attached membrane patches two types of single inward-rectifying K+ channels were observed, with single channel conductances of 7 and 35 pS. Cell-attached patches were also obtained at the antiluminal membrane of intact isolated cerebral capillaries. Only one type of K+ channel withg=30 pS was recorded. In conclusion, inwardly rectifying K+ channels, which can be inhibited by extracellular angiotensin II and arginine-vasopressin, are present in cerebral capillary endothelial cells. The inhibition of this K+ conductance by GTP[γ-S] indicates that G-proteins are involved in channel regulation. It is suggested that angiotensin II and vasopressin regulate K+ transport across the blood-brain barrier, mediating their effects via G-proteins.
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Keywords {🔍}

google, scholar, pubmed, article, brain, physiol, angiotensin, channels, endothelial, cells, potassium, membrane, inwardrectifying, channel, cerebral, current, vasopressin, bloodbrain, res, biol, capillary, isolated, single, currents, barrier, rat, bovine, goldstein, privacy, cookies, content, journal, meyer, capillaries, inhibition, transport, cell, betz, cultured, sci, usa, publish, search, gtpγs, porcine, hoyer, galla, gögelein, culture, access,

Topics {✒️}

month download article/chapter guanine nucleotide-binding protein guinea-pig small intestine hormone-stimulated polyphosphoinositide breakdown improved patch-clamp techniques amiloride-sensitive cationic channel bradykinin-induced potassium current voltage-dependent ionic channel cell-free membrane patches voltage-activated currents recorded guinea-pig ventricular myocytes cell-attached membrane patches γ-glutamyl transpeptidase activity high-resolution current recordings receptor-operated channels single-channel currents recorded guinea-pig heart potassium channel activity cyclic amp production coronary smooth muscle cultured endothelial cells extracellular angiotensin ii privacy choices/manage cookies bovine cerebral cortex rectifying single-channel currents coupling angiotensin receptors cell-attached patches membrane biology aims γ-glutamyl transpeptidase full article pdf mast cell line potassium channels endothelium-dependent relaxations canine basilar artery cerebral blood vessels blood-brain barrier steady-state current patch-clamp study article hoyer porcine brain capillaries single channel conductances single channel recording time-dependent decay brain microvascular endothelium histamin-secreting cells insulin-secreting cells anomalous potassium rectifier cultured rat myotubes brain water permeability rat cerebral arterioles

Schema {🗺️}

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         headline:Angiotensin II, vasopressin and GTP[γ-S] inhibit inward-rectifying K+ channels in porcine cerebral capillary endothelial cells
         description:Cerebral capillaries from porcine brain were isolated. and endothelial cells were grown in primary culture. The whole-cell tight seal patch-clamp method was applied to freshly isolated single endothelial cells, and cells which were held in culture up to one week. With high K+ solution in the patch pipette and in the bath we observed inward-rectifying K+ currents, showing a time-dependent decay in part of the experiments. Ba2+ (1–10mm) in the bath blocked this current, whereas outside tetraethylammonium (10mm) decreased the peak current but increased the steady-state current. Addition of 1 μm of angiotensin II or of arginine-vasopressin to the extracellular side caused a time-dependent inhibition of the inward-rectifying K+ current in part of the experiments. Addition of 100 μm GTP[γ-S] to the patch pipette blocked the K+ inward rectifier. In cell-attached membrane patches two types of single inward-rectifying K+ channels were observed, with single channel conductances of 7 and 35 pS. Cell-attached patches were also obtained at the antiluminal membrane of intact isolated cerebral capillaries. Only one type of K+ channel withg=30 pS was recorded. In conclusion, inwardly rectifying K+ channels, which can be inhibited by extracellular angiotensin II and arginine-vasopressin, are present in cerebral capillary endothelial cells. The inhibition of this K+ conductance by GTP[γ-S] indicates that G-proteins are involved in channel regulation. It is suggested that angiotensin II and vasopressin regulate K+ transport across the blood-brain barrier, mediating their effects via G-proteins.
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      headline:Angiotensin II, vasopressin and GTP[γ-S] inhibit inward-rectifying K+ channels in porcine cerebral capillary endothelial cells
      description:Cerebral capillaries from porcine brain were isolated. and endothelial cells were grown in primary culture. The whole-cell tight seal patch-clamp method was applied to freshly isolated single endothelial cells, and cells which were held in culture up to one week. With high K+ solution in the patch pipette and in the bath we observed inward-rectifying K+ currents, showing a time-dependent decay in part of the experiments. Ba2+ (1–10mm) in the bath blocked this current, whereas outside tetraethylammonium (10mm) decreased the peak current but increased the steady-state current. Addition of 1 μm of angiotensin II or of arginine-vasopressin to the extracellular side caused a time-dependent inhibition of the inward-rectifying K+ current in part of the experiments. Addition of 100 μm GTP[γ-S] to the patch pipette blocked the K+ inward rectifier. In cell-attached membrane patches two types of single inward-rectifying K+ channels were observed, with single channel conductances of 7 and 35 pS. Cell-attached patches were also obtained at the antiluminal membrane of intact isolated cerebral capillaries. Only one type of K+ channel withg=30 pS was recorded. In conclusion, inwardly rectifying K+ channels, which can be inhibited by extracellular angiotensin II and arginine-vasopressin, are present in cerebral capillary endothelial cells. The inhibition of this K+ conductance by GTP[γ-S] indicates that G-proteins are involved in channel regulation. It is suggested that angiotensin II and vasopressin regulate K+ transport across the blood-brain barrier, mediating their effects via G-proteins.
      datePublished:
      dateModified:
      pageStart:55
      pageEnd:62
      sameAs:https://doi.org/10.1007/BF01993963
      keywords:
         blood-brain barrier
         inward-rectifying K− channels
         angiotensin II
         arginine-vasopressin
         guanosine 5′-[γ-thio]triphosphate
         Biochemistry
         general
         Human Physiology
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