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We are analyzing https://link.springer.com/article/10.1007/bf01807363.

Title:
Biological differences among MCF-7 human breast cancer cell lines from different laboratories | Breast Cancer Research and Treatment
Description:
MCF-7 human breast cancer cells are used widely for studies of tumor biology and hormone mechanism of action. Conflicting results have often been obtained in studies reported from different laboratories. In this report several biological properties were studied in four MCF-7 cell lines obtained from different laboratories. MCF-7 (ATCC), MCF-7, MCF-7 (KO), and MCF-7 (S) demonstrated similar morphology in monolayer culture. Chromosome analysis revealed that three of the lines shared several structural chromosome alterations and marker chromsomes; however, MCF-7 (ATCC) was distinctly different with virtually no chromosomal alterations shared in common with the other lines. All four lines contained variable amounts of estrogen receptor (ER) and progesterone receptor (PgR). The growth rate of MCF-7 (ATCC) was 50% slower than that of the other lines, and, unlike the other three lines, cell proliferation was unaffected by estrogen or antiestrogen treatment despite the presence of receptors. Cloning efficiency of the four lines varied over a 10-fold range. Tumorigenicity in athymic nude mice also varied considerably among these lines. MCF-7 (ATCC) grew well in ovariectomized nude mice, while the other lines required estrogen supplementation. MCF-7 (S) and MCF-7 grew rapidly with estrogen supplementation; MCF-7 (KO) grew very slowly. Antiestrogen therapy inhibited growth of MCF-7, MCF-7 (S), and MCF-7 (KO) tumors, but it had no effect on MCF-7 (ATCC). These data demonstrate that MCF-7 lines from different laboratories may have unique biological properties, despite having a similar karyotype (MCF-7, MCF-7 (S), MCF-7 (KO)). The fundamental differences in karyotype and biological properties of the MCF-7 (ATCC), and the previously reported differences in DNA restriction fragment polymorphism analyses, demonstrate that this line is derived from an entirely different patient. Investigators should carefully document the source and identity of MCF-7 cells used in published experiments.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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  • Education
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Custom-built

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Traffic Estimate {πŸ“ˆ}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,626,432 visitors per month in the current month.

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How Does Link.springer.com Make Money? {πŸ’Έ}

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Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {πŸ”}

cancer, mcf, breast, google, scholar, human, pubmed, cell, res, lines, cells, estrogen, growth, article, osborne, culture, receptor, line, trent, atcc, privacy, cookies, research, biological, laboratories, effect, estrogens, content, analysis, data, publish, search, differences, hobbs, antiestrogen, nude, mice, access, mcguire, effects, tissue, usa, processing, information, log, journal, treatment, studies, properties, chromosome,

Topics {βœ’οΈ}

month download article/chapter human cell line cloning efficiency long-term tissue culture human breast cancer athymic nude mice ovariectomized nude mice cell line mcf-7 direct growth-stimulating effect estrogen-responsive cell chromosome analysis revealed chromosome banding analysis privacy choices/manage cookies restriction fragment polymorphisms tissue culture media human cytogenetic nomenclature breast cancer full article pdf epidermal growth factor european economic area scope submit manuscript structural chromosome alterations plasminogen activator activity de la garza breast carcinoma conditions privacy policy demonstrated similar morphology chromosomal alterations shared low serum concentration mcf-7 cells pleural effusion derived derived variant sublines previously reported differences cell genetics 21 accepting optional cookies usage analysis check access vitro model systems instant access unique biological properties cell proliferation similar karyotype mammalian cells estrogen receptor level estrogen receptor assays mcf-7 grew rapidly journal finder publish kent osborne cancer res 38 cancer res 44

Schema {πŸ—ΊοΈ}

WebPage:
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         headline:Biological differences among MCF-7 human breast cancer cell lines from different laboratories
         description:MCF-7 human breast cancer cells are used widely for studies of tumor biology and hormone mechanism of action. Conflicting results have often been obtained in studies reported from different laboratories. In this report several biological properties were studied in four MCF-7 cell lines obtained from different laboratories. MCF-7 (ATCC), MCF-7, MCF-7 (KO), and MCF-7 (S) demonstrated similar morphology in monolayer culture. Chromosome analysis revealed that three of the lines shared several structural chromosome alterations and marker chromsomes; however, MCF-7 (ATCC) was distinctly different with virtually no chromosomal alterations shared in common with the other lines. All four lines contained variable amounts of estrogen receptor (ER) and progesterone receptor (PgR). The growth rate of MCF-7 (ATCC) was 50% slower than that of the other lines, and, unlike the other three lines, cell proliferation was unaffected by estrogen or antiestrogen treatment despite the presence of receptors. Cloning efficiency of the four lines varied over a 10-fold range. Tumorigenicity in athymic nude mice also varied considerably among these lines. MCF-7 (ATCC) grew well in ovariectomized nude mice, while the other lines required estrogen supplementation. MCF-7 (S) and MCF-7 grew rapidly with estrogen supplementation; MCF-7 (KO) grew very slowly. Antiestrogen therapy inhibited growth of MCF-7, MCF-7 (S), and MCF-7 (KO) tumors, but it had no effect on MCF-7 (ATCC). These data demonstrate that MCF-7 lines from different laboratories may have unique biological properties, despite having a similar karyotype (MCF-7, MCF-7 (S), MCF-7 (KO)). The fundamental differences in karyotype and biological properties of the MCF-7 (ATCC), and the previously reported differences in DNA restriction fragment polymorphism analyses, demonstrate that this line is derived from an entirely different patient. Investigators should carefully document the source and identity of MCF-7 cells used in published experiments.
         datePublished:
         dateModified:
         pageStart:111
         pageEnd:121
         sameAs:https://doi.org/10.1007/BF01807363
         keywords:
            breast cancer
            cloning efficiency
            karyotype
            MCF-7 cells
            nude mice
            Oncology
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            name:Breast Cancer Research and Treatment
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               0167-6806
            volumeNumber:9
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            name:Martinus Nijhoff/Dr. W. Junk Publishers
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      headline:Biological differences among MCF-7 human breast cancer cell lines from different laboratories
      description:MCF-7 human breast cancer cells are used widely for studies of tumor biology and hormone mechanism of action. Conflicting results have often been obtained in studies reported from different laboratories. In this report several biological properties were studied in four MCF-7 cell lines obtained from different laboratories. MCF-7 (ATCC), MCF-7, MCF-7 (KO), and MCF-7 (S) demonstrated similar morphology in monolayer culture. Chromosome analysis revealed that three of the lines shared several structural chromosome alterations and marker chromsomes; however, MCF-7 (ATCC) was distinctly different with virtually no chromosomal alterations shared in common with the other lines. All four lines contained variable amounts of estrogen receptor (ER) and progesterone receptor (PgR). The growth rate of MCF-7 (ATCC) was 50% slower than that of the other lines, and, unlike the other three lines, cell proliferation was unaffected by estrogen or antiestrogen treatment despite the presence of receptors. Cloning efficiency of the four lines varied over a 10-fold range. Tumorigenicity in athymic nude mice also varied considerably among these lines. MCF-7 (ATCC) grew well in ovariectomized nude mice, while the other lines required estrogen supplementation. MCF-7 (S) and MCF-7 grew rapidly with estrogen supplementation; MCF-7 (KO) grew very slowly. Antiestrogen therapy inhibited growth of MCF-7, MCF-7 (S), and MCF-7 (KO) tumors, but it had no effect on MCF-7 (ATCC). These data demonstrate that MCF-7 lines from different laboratories may have unique biological properties, despite having a similar karyotype (MCF-7, MCF-7 (S), MCF-7 (KO)). The fundamental differences in karyotype and biological properties of the MCF-7 (ATCC), and the previously reported differences in DNA restriction fragment polymorphism analyses, demonstrate that this line is derived from an entirely different patient. Investigators should carefully document the source and identity of MCF-7 cells used in published experiments.
      datePublished:
      dateModified:
      pageStart:111
      pageEnd:121
      sameAs:https://doi.org/10.1007/BF01807363
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         breast cancer
         cloning efficiency
         karyotype
         MCF-7 cells
         nude mice
         Oncology
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            name:The University of Texas Health Science Center at San Antonio
            address:
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               type:PostalAddress
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      name:Kimberly Hobbs
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            name:The University of Texas Health Science Center at San Antonio
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               name:Department of Medicine Division of Oncology, The University of Texas Health Science Center at San Antonio, San Antonio, USA
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            name:University of Arizona Health Sciences Center
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               name:Cancer Center, University of Arizona Health Sciences Center, Tucson, USA
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      name:Department of Medicine Division of Oncology, The University of Texas Health Science Center at San Antonio, San Antonio, USA
      name:Department of Medicine Division of Oncology, The University of Texas Health Science Center at San Antonio, San Antonio, USA
      name:Cancer Center, University of Arizona Health Sciences Center, Tucson, USA
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