We are analyzing https://link.springer.com/article/10.1007/bf01806371.

Title:
The influence of growth factors on the proliferative potential of normal and primary breast cancer-derived human breast epithelial cells | Breast Cancer Research and Treatment
Description:
In previous studies, we developed serum-free, bovine pituitary extract (BPE)-free culture conditions for the growth of normal and neoplastic rat mammary epithelial cells. The present studies were aimed at determining if these culture methods could be used to study the influence of specific growth factors on the proliferative potential of normal human mammary epithelial (HME) cells and cells derived from human breast cancer (HBC) specimens. Our results indicate that normal HME cells in primary culture express stringent requirements for insulin (IN), epidermal growth factor (EGF), and cholera toxin (CT). Of these factors, EGF is most important, with essentially no proliferation taking place in the absence of this factor. By contrast, when cells are grown in serum-free primary culture in the presence of a full complement of growth factors and then subcultured, growth in secondary culture is not influenced by the removal of individual growth factors. Growth in secondary culture in the absence of EGF is mediated by autocrine factors secreted by the cells. However, there is no evidence for autocrine activity that mediates growth in the absence of IN in secondary cultures. Primary culture of HBC cells in serum-free, BPE-free medium revealed two patterns of growth factor requirements. One set of HBC cells expressed identical requirements for IN and EGF in primary culture as normal cells. Likewise, these cells grew in secondary culture in the absence of either factor. The second set of tumors expressed independence of IN for growth in primary culture. These cells grew to confluence in primary culture in the absence of IN and could be subcultured in this medium. All tumor cells examined expressed a requirement for EGF for primary culture growth, whereas none of the HBC cells examined expressed a significant CT requirement. In many cases, growth in the absence of CT exceeded that observed in its presence. Thus, our culture system allows analysis of the growth factor requirements of HME and HBC cells in primary culture. Our results indicate significant differences between HME and HBC cells in this regard. However, the results of secondary culture experiments indicate that the growth factor milieu from which cells are taken can have a profound effect on the requirements for growth factors in culture.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Careers
Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

growth, cells, culture, epithelial, google, scholar, normal, human, factor, pubmed, primary, mammary, breast, factors, cancer, cell, article, serumfree, res, ethier, stampfer, vitro, potential, rat, hbc, absence, clonal, privacy, cookies, research, cundiff, requirements, epidermal, egf, secondary, medium, expressed, usa, content, publish, search, influence, proliferative, hme, proliferation, access, analysis, data, information, log,

Topics {✒️}

cholera toxin stimulation epidermal growth factor supports long-term growth cholera toxin effector cell-independent effects human breast cancer autocrine factors secreted month download article/chapter growth factor requirements culture methods serum-free primary culture growth factor milieu growth factor interactions secondary cultures mouse epidermal keratinocytes serum-free culture conditions primary culture growth tumors expressed independence tumor growth modulation specific growth factors individual growth factors bpe-free medium revealed cells derived privacy choices/manage cookies insulin serum-free growth autocrine activity extended growth potential cell cycle studies check access instant access free culture conditions rapid clonal growth proliferation taking place developed serum-free normal hme cells growth factors conditions privacy policy european economic area michigan medical center full article pdf serum-free media serum-free medium secondary culture experiments primary culture accepting optional cookies bovine pituitary extract defined phenotypic traits extended serial passage normal cells

Schema {🗺️}

WebPage:
      mainEntity:
         headline:The influence of growth factors on the proliferative potential of normal and primary breast cancer-derived human breast epithelial cells
         description:In previous studies, we developed serum-free, bovine pituitary extract (BPE)-free culture conditions for the growth of normal and neoplastic rat mammary epithelial cells. The present studies were aimed at determining if these culture methods could be used to study the influence of specific growth factors on the proliferative potential of normal human mammary epithelial (HME) cells and cells derived from human breast cancer (HBC) specimens. Our results indicate that normal HME cells in primary culture express stringent requirements for insulin (IN), epidermal growth factor (EGF), and cholera toxin (CT). Of these factors, EGF is most important, with essentially no proliferation taking place in the absence of this factor. By contrast, when cells are grown in serum-free primary culture in the presence of a full complement of growth factors and then subcultured, growth in secondary culture is not influenced by the removal of individual growth factors. Growth in secondary culture in the absence of EGF is mediated by autocrine factors secreted by the cells. However, there is no evidence for autocrine activity that mediates growth in the absence of IN in secondary cultures. Primary culture of HBC cells in serum-free, BPE-free medium revealed two patterns of growth factor requirements. One set of HBC cells expressed identical requirements for IN and EGF in primary culture as normal cells. Likewise, these cells grew in secondary culture in the absence of either factor. The second set of tumors expressed independence of IN for growth in primary culture. These cells grew to confluence in primary culture in the absence of IN and could be subcultured in this medium. All tumor cells examined expressed a requirement for EGF for primary culture growth, whereas none of the HBC cells examined expressed a significant CT requirement. In many cases, growth in the absence of CT exceeded that observed in its presence. Thus, our culture system allows analysis of the growth factor requirements of HME and HBC cells in primary culture. Our results indicate significant differences between HME and HBC cells in this regard. However, the results of secondary culture experiments indicate that the growth factor milieu from which cells are taken can have a profound effect on the requirements for growth factors in culture.
         datePublished:
         dateModified:
         pageStart:221
         pageEnd:230
         sameAs:https://doi.org/10.1007/BF01806371
         keywords:
            autocrine factors
            cholera toxin
            culture methods
            epidermal growth factor
            growth factor
            insulin
            mammary epithelial cells
            primary cultures
            Oncology
         image:
         isPartOf:
            name:Breast Cancer Research and Treatment
            issn:
               1573-7217
               0167-6806
            volumeNumber:17
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Kluwer Academic Publishers
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Stephen P. Ethier
               affiliation:
                     name:The University of Michigan Medical Center
                     address:
                        name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Rochelle M. Summerfelt
               affiliation:
                     name:The University of Michigan Medical Center
                     address:
                        name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kimberly C. Cundiff
               affiliation:
                     name:The University of Michigan Medical Center
                     address:
                        name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bonnie B. Asch
               affiliation:
                     name:Roswell Park Memorial Institute
                     address:
                        name:Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:The influence of growth factors on the proliferative potential of normal and primary breast cancer-derived human breast epithelial cells
      description:In previous studies, we developed serum-free, bovine pituitary extract (BPE)-free culture conditions for the growth of normal and neoplastic rat mammary epithelial cells. The present studies were aimed at determining if these culture methods could be used to study the influence of specific growth factors on the proliferative potential of normal human mammary epithelial (HME) cells and cells derived from human breast cancer (HBC) specimens. Our results indicate that normal HME cells in primary culture express stringent requirements for insulin (IN), epidermal growth factor (EGF), and cholera toxin (CT). Of these factors, EGF is most important, with essentially no proliferation taking place in the absence of this factor. By contrast, when cells are grown in serum-free primary culture in the presence of a full complement of growth factors and then subcultured, growth in secondary culture is not influenced by the removal of individual growth factors. Growth in secondary culture in the absence of EGF is mediated by autocrine factors secreted by the cells. However, there is no evidence for autocrine activity that mediates growth in the absence of IN in secondary cultures. Primary culture of HBC cells in serum-free, BPE-free medium revealed two patterns of growth factor requirements. One set of HBC cells expressed identical requirements for IN and EGF in primary culture as normal cells. Likewise, these cells grew in secondary culture in the absence of either factor. The second set of tumors expressed independence of IN for growth in primary culture. These cells grew to confluence in primary culture in the absence of IN and could be subcultured in this medium. All tumor cells examined expressed a requirement for EGF for primary culture growth, whereas none of the HBC cells examined expressed a significant CT requirement. In many cases, growth in the absence of CT exceeded that observed in its presence. Thus, our culture system allows analysis of the growth factor requirements of HME and HBC cells in primary culture. Our results indicate significant differences between HME and HBC cells in this regard. However, the results of secondary culture experiments indicate that the growth factor milieu from which cells are taken can have a profound effect on the requirements for growth factors in culture.
      datePublished:
      dateModified:
      pageStart:221
      pageEnd:230
      sameAs:https://doi.org/10.1007/BF01806371
      keywords:
         autocrine factors
         cholera toxin
         culture methods
         epidermal growth factor
         growth factor
         insulin
         mammary epithelial cells
         primary cultures
         Oncology
      image:
      isPartOf:
         name:Breast Cancer Research and Treatment
         issn:
            1573-7217
            0167-6806
         volumeNumber:17
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Kluwer Academic Publishers
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Stephen P. Ethier
            affiliation:
                  name:The University of Michigan Medical Center
                  address:
                     name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Rochelle M. Summerfelt
            affiliation:
                  name:The University of Michigan Medical Center
                  address:
                     name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kimberly C. Cundiff
            affiliation:
                  name:The University of Michigan Medical Center
                  address:
                     name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bonnie B. Asch
            affiliation:
                  name:Roswell Park Memorial Institute
                  address:
                     name:Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Breast Cancer Research and Treatment
      issn:
         1573-7217
         0167-6806
      volumeNumber:17
Organization:
      name:Kluwer Academic Publishers
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:The University of Michigan Medical Center
      address:
         name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
         type:PostalAddress
      name:The University of Michigan Medical Center
      address:
         name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
         type:PostalAddress
      name:The University of Michigan Medical Center
      address:
         name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
         type:PostalAddress
      name:Roswell Park Memorial Institute
      address:
         name:Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Stephen P. Ethier
      affiliation:
            name:The University of Michigan Medical Center
            address:
               name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
               type:PostalAddress
            type:Organization
      name:Rochelle M. Summerfelt
      affiliation:
            name:The University of Michigan Medical Center
            address:
               name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
               type:PostalAddress
            type:Organization
      name:Kimberly C. Cundiff
      affiliation:
            name:The University of Michigan Medical Center
            address:
               name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
               type:PostalAddress
            type:Organization
      name:Bonnie B. Asch
      affiliation:
            name:Roswell Park Memorial Institute
            address:
               name:Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
      name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
      name:Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor, USA
      name:Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(67)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.56s.

LINK . SPRINGER . COM {}