We are analyzing https://link.springer.com/article/10.1007/bf01209122.

Title:
Effect of oestradiol and insulin on the proliferative pattern and on oestrogen and progesterone receptor contents in MCF-7 cells | Journal of Cancer Research and Clinical Oncology
Description:
In a long-term experiment MCF-7 cells showed an exponential proliferation rate in fetal calf serum while they become quiescent in growth-factor-and steroid-free serum. The mitogenic activity of this cell line was increased by oestradiol and insulin, exposure to both hormones showing a synergic effect. These mitogen-mediated effects are inhibited by genistein a phytoestrogen which, by itself at the doses used, did not affect either the basal proliferation rate or the total protein content. Immunoblotting and Scatchard analysis reveal respectively that insulin increases the oestrogen receptor (ER) content and its binding capacity. The presence of genistein decreases the ER content and completely abolishes the binding capacity of this protein. Insulin is able to increase progresterone receptor (PR) levels while, in the presence of genistein, the above-reported effect was completely abolished. On the basis of the latter data the authors suggest that insulin is able to affect the phosphorylation status of ER and PR, inducing functional changes in both proteins, although this was in the absence of their natural ligands. Indeed, the presence in the medium of a tyrosine kinase inhibitor such as genistein could substantially decrease both ER and PR levels in these cells.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Social Networks
Science
Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't see how the site brings in money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

receptor, google, scholar, article, cells, insulin, content, cancer, genistein, tyrosine, growth, progesterone, mcf, access, privacy, cookies, journal, research, oestrogen, serum, activity, cell, human, estrogen, data, publish, search, effect, kinase, receptors, factor, breast, van, information, log, oncology, oestradiol, proliferative, panno, salerno, pezzi, proliferation, effects, protein, binding, presence, phosphorylation, medium, open, discover,

Topics {✒️}

month download article/chapter tyrosine kinase inhibitor growth factor-defined medium growth-factor-defined medium fetal calf serum tyrosine kinase activity related subjects breast cancer cells full article pdf mitogen-mediated effects general metabolic effects privacy choices/manage cookies clinical oncology aims tyrosine 537in vivo progesterone receptor contents steroid-free serum mcf-7 cells growth factor receptor human estrogen receptor receptors type 1 insulin check access arcavacata di rende instant access european economic area scope submit manuscript exponential proliferation rate basal proliferation rate darnell je jr laat sw de naturally-occurring estrogens università della calabria increase progresterone receptor conditions privacy policy cancer research scatchard analysis reveal dietary intervention study accepting optional cookies december 1996 volume 122 hughes cl jr estrogen-stimulated proliferation journal finder publish article journal estrogen receptor status progesterone receptor growth-factor growth factor article log total protein content andò rights

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Effect of oestradiol and insulin on the proliferative pattern and on oestrogen and progesterone receptor contents in MCF-7 cells
         description:In a long-term experiment MCF-7 cells showed an exponential proliferation rate in fetal calf serum while they become quiescent in growth-factor-and steroid-free serum. The mitogenic activity of this cell line was increased by oestradiol and insulin, exposure to both hormones showing a synergic effect. These mitogen-mediated effects are inhibited by genistein a phytoestrogen which, by itself at the doses used, did not affect either the basal proliferation rate or the total protein content. Immunoblotting and Scatchard analysis reveal respectively that insulin increases the oestrogen receptor (ER) content and its binding capacity. The presence of genistein decreases the ER content and completely abolishes the binding capacity of this protein. Insulin is able to increase progresterone receptor (PR) levels while, in the presence of genistein, the above-reported effect was completely abolished. On the basis of the latter data the authors suggest that insulin is able to affect the phosphorylation status of ER and PR, inducing functional changes in both proteins, although this was in the absence of their natural ligands. Indeed, the presence in the medium of a tyrosine kinase inhibitor such as genistein could substantially decrease both ER and PR levels in these cells.
         datePublished:
         dateModified:
         pageStart:745
         pageEnd:749
         sameAs:https://doi.org/10.1007/BF01209122
         keywords:
            Oestrogen receptors
            Progesterone receptors
            MCF-7
            Genistein
            Tyrosine kinase inhibitor
            Oncology
            Cancer Research
            Internal Medicine
            Hematology
         image:
         isPartOf:
            name:Journal of Cancer Research and Clinical Oncology
            issn:
               1432-1335
               0171-5216
            volumeNumber:122
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:M. L. Panno
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:M. Salerno
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:V. Pezzi
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:D. Sisci
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:M. Maggiolini
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:L. Mauro
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:E. G. Morrone
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:S. Andò
               affiliation:
                     name:Università della Calabria
                     address:
                        name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Effect of oestradiol and insulin on the proliferative pattern and on oestrogen and progesterone receptor contents in MCF-7 cells
      description:In a long-term experiment MCF-7 cells showed an exponential proliferation rate in fetal calf serum while they become quiescent in growth-factor-and steroid-free serum. The mitogenic activity of this cell line was increased by oestradiol and insulin, exposure to both hormones showing a synergic effect. These mitogen-mediated effects are inhibited by genistein a phytoestrogen which, by itself at the doses used, did not affect either the basal proliferation rate or the total protein content. Immunoblotting and Scatchard analysis reveal respectively that insulin increases the oestrogen receptor (ER) content and its binding capacity. The presence of genistein decreases the ER content and completely abolishes the binding capacity of this protein. Insulin is able to increase progresterone receptor (PR) levels while, in the presence of genistein, the above-reported effect was completely abolished. On the basis of the latter data the authors suggest that insulin is able to affect the phosphorylation status of ER and PR, inducing functional changes in both proteins, although this was in the absence of their natural ligands. Indeed, the presence in the medium of a tyrosine kinase inhibitor such as genistein could substantially decrease both ER and PR levels in these cells.
      datePublished:
      dateModified:
      pageStart:745
      pageEnd:749
      sameAs:https://doi.org/10.1007/BF01209122
      keywords:
         Oestrogen receptors
         Progesterone receptors
         MCF-7
         Genistein
         Tyrosine kinase inhibitor
         Oncology
         Cancer Research
         Internal Medicine
         Hematology
      image:
      isPartOf:
         name:Journal of Cancer Research and Clinical Oncology
         issn:
            1432-1335
            0171-5216
         volumeNumber:122
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:M. L. Panno
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M. Salerno
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:V. Pezzi
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:D. Sisci
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M. Maggiolini
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:L. Mauro
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:E. G. Morrone
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:S. Andò
            affiliation:
                  name:Università della Calabria
                  address:
                     name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Journal of Cancer Research and Clinical Oncology
      issn:
         1432-1335
         0171-5216
      volumeNumber:122
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
      name:Università della Calabria
      address:
         name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:M. L. Panno
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:M. Salerno
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:V. Pezzi
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:D. Sisci
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:M. Maggiolini
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:L. Mauro
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:E. G. Morrone
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
      name:S. Andò
      affiliation:
            name:Università della Calabria
            address:
               name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
      name:Cattedra di Fisiopatologia Endocrina, Dipartimento di Biologia Cellulare, Università della Calabria, Cosenza, Italy
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(53)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.05s.

LINK . SPRINGER . COM {}