We are analyzing https://link.springer.com/article/10.1007/bf01209032.

Title:
Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients | Journal of Cancer Research and Clinical Oncology
Description:
A two-step reverse-transcriptase-based polymerase chain reaction (PCR) with nested primer pairs was developed to amplify sensitive and specific cytokeratin-19 (CK-19) mRNA sequences from human breast cancer cells. No CK-19 pseudogene interference was seen. The larger DNA-derived amplification products could be clearly discriminated from mRNA-derived products. The CK-19 message was not amplified from bone marrow or blood of healthy volunteers and patients with haematological malignancies nor from myeloid and lymphoid cell lines. Breast cancer cells were diluted in buffy coat cells up to 10−6 and CK-19 mRNA sought by PCR. The CK-19 message was detected in 14 of 26 blood samples and 14 of 24 marrow samples but in neither of two peripheral blood stem cell samples taken from 35 breast cancer patients. By sequence-analysis control of two of these samples and two cell lines, the amplified DNA fragments were confirmed to be homologous with the CK-19 sequence. The CK-19 message was further sought in matched blood/marrow samples taken from 13 untreated women in the same cohort at the time of diagnosis. In 3 of these, CK-19 RNA was detected in blood and marrow and, in 3 others, only in blood, but never in marrow alone. The results show that CK-19 assay by reverse transcriptase/PCR is a sensitive and specific technique for the detection of cancer cells in bone marrow and blood. It could be helpful in diagnosis and monitoring of metastatic breast cancer and detection of micrometastases. This should be evaluated on larger numbers of patients, with different clinical samples and epithelial malignancies.
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Keywords {🔍}

cancer, google, scholar, pubmed, breast, marrow, detection, bone, cells, blood, chain, reaction, patients, article, cell, oncol, clin, polymerase, reverse, human, samples, privacy, cookies, content, journal, research, clinical, cytokeratin, specific, peripheral, stem, sequence, micrometastases, tumor, publish, search, oncology, mrna, krüger, products, dna, access, res, germany, data, information, log, transcriptasepolymerase, krzizanowski, holweg,

Topics {✒️}

minimal residual disease polymerase-catalyzed chain reaction month download article/chapter polymerase chain reaction monoclonal antibody-defined epitopes direct automated sequencing reverse transcriptase/pcr anti-17-1a monoclonal antibodies high-dose consolidation therapy human beta-actin cdna clinical oncology aims matched blood/marrow samples cold spring harbor reverse transcriptase breast cancer micrometastases tumor cell contamination mrna-derived products privacy choices/manage cookies advanced breast cancer breast cancer cells amino acid sequence haematological malignancies gene organization autologous marrow support operable breast cancer occult breast cancer primary breast cancer full article pdf epithelial specific rna single-step method möglichkeiten der immunzytochemie proc annu meet metastatic breast cancer breast cancer patients micrometastatic cancer cells related subjects bone marrow aspirates high-dose cyclophosphamide standard-dose therapy epithelial cancer cells lymphoid cell lines ck-19 pseudogene interference dose-response effect buffy coat cells ck-19 rna european economic area nested primer pairs teicher ba critchlow intermediate filament protein university-hospital eppendorf

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         headline:Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients
         description:A two-step reverse-transcriptase-based polymerase chain reaction (PCR) with nested primer pairs was developed to amplify sensitive and specific cytokeratin-19 (CK-19) mRNA sequences from human breast cancer cells. No CK-19 pseudogene interference was seen. The larger DNA-derived amplification products could be clearly discriminated from mRNA-derived products. The CK-19 message was not amplified from bone marrow or blood of healthy volunteers and patients with haematological malignancies nor from myeloid and lymphoid cell lines. Breast cancer cells were diluted in buffy coat cells up to 10−6 and CK-19 mRNA sought by PCR. The CK-19 message was detected in 14 of 26 blood samples and 14 of 24 marrow samples but in neither of two peripheral blood stem cell samples taken from 35 breast cancer patients. By sequence-analysis control of two of these samples and two cell lines, the amplified DNA fragments were confirmed to be homologous with the CK-19 sequence. The CK-19 message was further sought in matched blood/marrow samples taken from 13 untreated women in the same cohort at the time of diagnosis. In 3 of these, CK-19 RNA was detected in blood and marrow and, in 3 others, only in blood, but never in marrow alone. The results show that CK-19 assay by reverse transcriptase/PCR is a sensitive and specific technique for the detection of cancer cells in bone marrow and blood. It could be helpful in diagnosis and monitoring of metastatic breast cancer and detection of micrometastases. This should be evaluated on larger numbers of patients, with different clinical samples and epithelial malignancies.
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            Minimal residual disease
            RT-PCR
            Breast cancer
            Oncology
            Cancer Research
            Internal Medicine
            Hematology
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      headline:Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients
      description:A two-step reverse-transcriptase-based polymerase chain reaction (PCR) with nested primer pairs was developed to amplify sensitive and specific cytokeratin-19 (CK-19) mRNA sequences from human breast cancer cells. No CK-19 pseudogene interference was seen. The larger DNA-derived amplification products could be clearly discriminated from mRNA-derived products. The CK-19 message was not amplified from bone marrow or blood of healthy volunteers and patients with haematological malignancies nor from myeloid and lymphoid cell lines. Breast cancer cells were diluted in buffy coat cells up to 10−6 and CK-19 mRNA sought by PCR. The CK-19 message was detected in 14 of 26 blood samples and 14 of 24 marrow samples but in neither of two peripheral blood stem cell samples taken from 35 breast cancer patients. By sequence-analysis control of two of these samples and two cell lines, the amplified DNA fragments were confirmed to be homologous with the CK-19 sequence. The CK-19 message was further sought in matched blood/marrow samples taken from 13 untreated women in the same cohort at the time of diagnosis. In 3 of these, CK-19 RNA was detected in blood and marrow and, in 3 others, only in blood, but never in marrow alone. The results show that CK-19 assay by reverse transcriptase/PCR is a sensitive and specific technique for the detection of cancer cells in bone marrow and blood. It could be helpful in diagnosis and monitoring of metastatic breast cancer and detection of micrometastases. This should be evaluated on larger numbers of patients, with different clinical samples and epithelial malignancies.
      datePublished:
      dateModified:
      pageStart:679
      pageEnd:686
      sameAs:https://doi.org/10.1007/BF01209032
      keywords:
         Micrometastases
         Minimal residual disease
         RT-PCR
         Breast cancer
         Oncology
         Cancer Research
         Internal Medicine
         Hematology
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