We are analyzing https://link.springer.com/article/10.1007/bf01002772.

Title:
Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections | Journal of Molecular Histology
Description:
Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency. Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.
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Keywords {🔍}

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Topics {✒️}

month download article/chapter sulphonic acid groups picrosirius red-polarization detection sialic acid carboxyls acid dye binding privacy choices/manage cookies related subjects collagen fibre full article pdf polarization microscopic studies connective tissue stained faculdade de medicina human dermal collagen histochemical journal aims polarization microscopy picrosirius red f3ba european economic area scope submit manuscript basic groups present pesquisa em reumatologia conditions privacy policy strong anionic dye elongated dye molecules sirius red f3ba check access instant access accepting optional cookies epidermal structural proteins journal finder publish tissue sections main content log connective tissue fibrillar collagen collagen detection brentani rights article junqueira article log privacy policy personal data books a sirius red stains collagen collagen molecule collagen results collagen fibers collagen structures article cite histochemical study picrosirius staining optional cookies

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