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Title:
Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus | Molecular Genetics and Genomics
Description:
Carotenoid pigments are essential for the protection of both photosynthetic and non-photosynthetic tissues from photooxidative damage. Although carotenoid biosynthesis has been studied in many organisms from bacteria to higher plants, little is known about carotenoid biosynthetic enzymes, or the nature and regulation of the genes encoding them. We report here the first DNA sequence of carotenoid genes from any organism. We have determined the complete nucleotide sequence (11 039 bp) of a gene cluster encoding seven of the eight previously known carotenoid genes (crt A, B, C, D, E, F, I) and a new gene, designated crtK, from Rhodobacter capsulatus, a purple non-sulfur photosynthetic bacterium. The 5β² flanking regions of crtA, I, D and E contain a highly conserved palindromic sequence homologous to the consensus binding site for a variety of prokaryotic DNA-binding regulatory proteins. This putative regulatory palindrome is also found 5β² to the puc operon, encoding the light-harvesting II antenna polypeptides. Escherichia coli-like Ο70 promoter sequences are located 5β² to crtI and crtD, suggesting for the first time that such promoters may exist in purple photosynthetic bacteria. The crt genes form a minimum of four distinct operons, crtA, crtIBK, crtDC and crtEF, based on inversions of transcriptional orientation within the gene cluster. Possible rho-independent transcription terminators are located 3β² to crtI, B, K, C and F. The 3β² end of crtA may overlap transcription initiation signals for a downstream gene required for bacteriochlorophyll biosynthesis. We have also observed two regions of exceptional amino acid homology between CrtI and CrtD, both of which are dehydrogenases.
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Keywords {π}
google, scholar, genes, biosynthesis, carotenoid, gene, rhodopseudomonas, rhodobacter, capsulata, sequence, capsulatus, article, photosynthetic, dna, usa, rev, bacteria, transcription, carotenoids, bacteriol, hearst, lightharvesting, sci, expression, regulation, marrs, cloning, proc, natl, acad, annu, nucleotide, nature, cluster, alberti, access, sphaeroides, genet, privacy, cookies, content, research, armstrong, enzymes, initiation, biol, biochem, reaction, mol, microbiol,
Topics {βοΈ}
month download article/chapter bacterial photosynthetic apparatus rho-independent transcription terminators duplicated nifa/nifb region cold spring harbor post-transcriptional regulation membrane-bound carotenogenic enzymes light-harvesting genes b800-850-Ξ² genes pigment-binding proteins privacy choices/manage cookies light-harvesting complexes conditions privacy policy carotenoid biosynthesis genes full article pdf light-harvesting ii b800-b850 complex rhodobacter capsulatus published nitrogen fixation genes shotgun dna sequencing dna fragment hybridizing putative regulatory palindrome sequencing plasmid dna reaction center complexes complete nucleotide sequence escherichia coli reaction center polypeptides escherichia coli escherichia coli 16 steady-state levels puf mrna stability sulfur photosynthetic bacterium crt genes form carotenoid biosynthetic enzymes european economic area marr-type regulator photochemical reaction centers molecular genetics aphanocapsa homogenates coupled chain terminating inhibitors dimensional protein structure related subjects photosynthetic gene cluster Ο70 promoter sequences plasmid pbr322 carrying upstream activator sequences deduced polypeptide sequences mrna decay terminator photosynthetic reaction-center ribosome binding sites
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headline:Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus
description:Carotenoid pigments are essential for the protection of both photosynthetic and non-photosynthetic tissues from photooxidative damage. Although carotenoid biosynthesis has been studied in many organisms from bacteria to higher plants, little is known about carotenoid biosynthetic enzymes, or the nature and regulation of the genes encoding them. We report here the first DNA sequence of carotenoid genes from any organism. We have determined the complete nucleotide sequence (11 039 bp) of a gene cluster encoding seven of the eight previously known carotenoid genes (crt A, B, C, D, E, F, I) and a new gene, designated crtK, from Rhodobacter capsulatus, a purple non-sulfur photosynthetic bacterium. The 5β² flanking regions of crtA, I, D and E contain a highly conserved palindromic sequence homologous to the consensus binding site for a variety of prokaryotic DNA-binding regulatory proteins. This putative regulatory palindrome is also found 5β² to the puc operon, encoding the light-harvesting II antenna polypeptides. Escherichia coli-like Ο70 promoter sequences are located 5β² to crtI and crtD, suggesting for the first time that such promoters may exist in purple photosynthetic bacteria. The crt genes form a minimum of four distinct operons, crtA, crtIBK, crtDC and crtEF, based on inversions of transcriptional orientation within the gene cluster. Possible rho-independent transcription terminators are located 3β² to crtI, B, K, C and F. The 3β² end of crtA may overlap transcription initiation signals for a downstream gene required for bacteriochlorophyll biosynthesis. We have also observed two regions of exceptional amino acid homology between CrtI and CrtD, both of which are dehydrogenases.
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description:Carotenoid pigments are essential for the protection of both photosynthetic and non-photosynthetic tissues from photooxidative damage. Although carotenoid biosynthesis has been studied in many organisms from bacteria to higher plants, little is known about carotenoid biosynthetic enzymes, or the nature and regulation of the genes encoding them. We report here the first DNA sequence of carotenoid genes from any organism. We have determined the complete nucleotide sequence (11 039 bp) of a gene cluster encoding seven of the eight previously known carotenoid genes (crt A, B, C, D, E, F, I) and a new gene, designated crtK, from Rhodobacter capsulatus, a purple non-sulfur photosynthetic bacterium. The 5β² flanking regions of crtA, I, D and E contain a highly conserved palindromic sequence homologous to the consensus binding site for a variety of prokaryotic DNA-binding regulatory proteins. This putative regulatory palindrome is also found 5β² to the puc operon, encoding the light-harvesting II antenna polypeptides. Escherichia coli-like Ο70 promoter sequences are located 5β² to crtI and crtD, suggesting for the first time that such promoters may exist in purple photosynthetic bacteria. The crt genes form a minimum of four distinct operons, crtA, crtIBK, crtDC and crtEF, based on inversions of transcriptional orientation within the gene cluster. Possible rho-independent transcription terminators are located 3β² to crtI, B, K, C and F. The 3β² end of crtA may overlap transcription initiation signals for a downstream gene required for bacteriochlorophyll biosynthesis. We have also observed two regions of exceptional amino acid homology between CrtI and CrtD, both of which are dehydrogenases.
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Rhodobacter capsulatus
Transcriptional regulation
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Escherichia coli-like promoters
Plant Genetics and Genomics
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Microbial Genetics and Genomics
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