We are analyzing https://link.springer.com/article/10.1007/bf00323577.

Title:
A morphological and immunohistochemical study of programmed cell death in Botryllus schlosseri (Tunicata, Ascidiacea) | Cell and Tissue Research
Description:
The blastogenic cycle of the colonial ascidian Botryllus schlosseri concludes in a phase of selective cell and zooid death called takeover. Every week, all asexually derived parental zooids synchronously regress over a 30-h period and are replaced by a new generation. Here we document the sequential ultrastructural changes which accompany cell death during zooid degeneration. The principal mode of visceral cell death during takeover occurred by apoptosis, the majority of cells condensing and fragmenting into multiple membrane-bounded apoptotic bodies. Cytoplasmic organelles (mitochondria, basal bodies, striated rootlets) within apoptotic bodies retained ultrastructural integrity. Dying cells and fragments were then swiftly ingested by specialized blood macrophages or intraepithelial phagocytes and subsequently underwent secondary necrotic lysis. Certain organs (stomach, intestine) displayed a combination of necrotic and apoptotic changes. Lastly, the stomach, which demonstrated some of the earliest regressive changes, exhibited intense cytoplasmic immunostaining with a monoclonal antibody to ubiquitin at the onset of takeover. Affinity-purified rabbit antiserum against sodium dodecyl sulfate-denatured ubiquitin detected a characteristic 8.6-kDa mono-ubiquitin band by Western blot analysis. Collectively, these findings raise the possibility that cell death during takeover is a dynamic process which requires active participation of cells in their own destruction.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Telecommunications
Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We see no obvious way the site makes money.

While many websites aim to make money, others are created to share knowledge or showcase creativity. People build websites for various reasons. This could be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

google, scholar, cell, death, apoptosis, programmed, article, cells, botryllus, usa, weissman, sci, nature, schlosseri, wyllie, research, tissue, lauzon, ubiquitin, access, biol, exp, privacy, cookies, content, study, ultrastructural, protein, immunol, publish, search, colonial, takeover, metamorphosis, morphol, science, dna, fragmentation, proc, natl, acad, embryol, rev, target, albany, medical, data, information, log, journal,

Topics {✒️}

month download article/chapter 6-kda mono-ubiquitin band vitronectin receptor-mediated phagocytosis radiation-induced interphase death affinity-purified rabbit antiserum hormone-induced cell death intracellular ubiquitin-protein conjugates targeted short-lived protein ubiquitin-mediated protein degradation cd4+cd8+tcrlo thymocytes activation-induced cell death glucocorticoid-induced thymocyte apoptosis basic biological phenomenon cytolytic lymphocytes induce specialized blood macrophages botryllus schlosseri pallas privacy choices/manage cookies programmed cell death beckman research center full article pdf accompany cell death visceral cell death cell-mediated cytolysis tissue research aims endogenous suicide process endogenous endonuclease activation european economic area requires active participation related subjects posterior necrotic zone wide-ranging implications ventral nerve cord thymocytes undergoing apoptosis conditions privacy policy western blot analysis mitophagy response leads chick wing-bud striated muscle fibers walport mj polyubiquitin gene expression albany medical college caenorhabditis elegans larvae botryllus schlosseri electron-microscope study accepting optional cookies autoreactive blood cells anuran tadpole tail cells undergoing apoptosis cell sci 14 main content log

Questions {❓}

A cell’s agony of choice: how to cross the Styx?
Weissman IL, Saito Y, Rinkevich B (1990) Allorecognition histocompatibility in a protochordate species: is the relationship to MHC semantic or structural?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:A morphological and immunohistochemical study of programmed cell death in Botryllus schlosseri (Tunicata, Ascidiacea)
         description:The blastogenic cycle of the colonial ascidian Botryllus schlosseri concludes in a phase of selective cell and zooid death called takeover. Every week, all asexually derived parental zooids synchronously regress over a 30-h period and are replaced by a new generation. Here we document the sequential ultrastructural changes which accompany cell death during zooid degeneration. The principal mode of visceral cell death during takeover occurred by apoptosis, the majority of cells condensing and fragmenting into multiple membrane-bounded apoptotic bodies. Cytoplasmic organelles (mitochondria, basal bodies, striated rootlets) within apoptotic bodies retained ultrastructural integrity. Dying cells and fragments were then swiftly ingested by specialized blood macrophages or intraepithelial phagocytes and subsequently underwent secondary necrotic lysis. Certain organs (stomach, intestine) displayed a combination of necrotic and apoptotic changes. Lastly, the stomach, which demonstrated some of the earliest regressive changes, exhibited intense cytoplasmic immunostaining with a monoclonal antibody to ubiquitin at the onset of takeover. Affinity-purified rabbit antiserum against sodium dodecyl sulfate-denatured ubiquitin detected a characteristic 8.6-kDa mono-ubiquitin band by Western blot analysis. Collectively, these findings raise the possibility that cell death during takeover is a dynamic process which requires active participation of cells in their own destruction.
         datePublished:
         dateModified:
         pageStart:115
         pageEnd:127
         sameAs:https://doi.org/10.1007/BF00323577
         keywords:
            Apoptosis
            Necrosis
            Programmed cell death
            Macrophages
            Ubiquitin
             Botryllus schlosseri (Tunicata)
            Human Genetics
            Proteomics
            Molecular Medicine
         image:
         isPartOf:
            name:Cell and Tissue Research
            issn:
               1432-0878
               0302-766X
            volumeNumber:272
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Robert J. Lauzon
               affiliation:
                     name:Albany Medical College
                     address:
                        name:Department of Pediatrics, Albany Medical College, Albany, USA
                        type:PostalAddress
                     type:Organization
                     name:Albany Medical College
                     address:
                        name:Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, Albany, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chris W. Patton
               affiliation:
                     name:Hopkins Marine Station of Stanford University
                     address:
                        name:Hopkins Marine Station of Stanford University, Pacific Grove, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Irving L. Weissman
               affiliation:
                     name:Stanford University Medical Center
                     address:
                        name:Department of Pathology and Developmental Biology, Howard Hughes Medical Institute, Beckman Research Center, Stanford University Medical Center, Stanford, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:A morphological and immunohistochemical study of programmed cell death in Botryllus schlosseri (Tunicata, Ascidiacea)
      description:The blastogenic cycle of the colonial ascidian Botryllus schlosseri concludes in a phase of selective cell and zooid death called takeover. Every week, all asexually derived parental zooids synchronously regress over a 30-h period and are replaced by a new generation. Here we document the sequential ultrastructural changes which accompany cell death during zooid degeneration. The principal mode of visceral cell death during takeover occurred by apoptosis, the majority of cells condensing and fragmenting into multiple membrane-bounded apoptotic bodies. Cytoplasmic organelles (mitochondria, basal bodies, striated rootlets) within apoptotic bodies retained ultrastructural integrity. Dying cells and fragments were then swiftly ingested by specialized blood macrophages or intraepithelial phagocytes and subsequently underwent secondary necrotic lysis. Certain organs (stomach, intestine) displayed a combination of necrotic and apoptotic changes. Lastly, the stomach, which demonstrated some of the earliest regressive changes, exhibited intense cytoplasmic immunostaining with a monoclonal antibody to ubiquitin at the onset of takeover. Affinity-purified rabbit antiserum against sodium dodecyl sulfate-denatured ubiquitin detected a characteristic 8.6-kDa mono-ubiquitin band by Western blot analysis. Collectively, these findings raise the possibility that cell death during takeover is a dynamic process which requires active participation of cells in their own destruction.
      datePublished:
      dateModified:
      pageStart:115
      pageEnd:127
      sameAs:https://doi.org/10.1007/BF00323577
      keywords:
         Apoptosis
         Necrosis
         Programmed cell death
         Macrophages
         Ubiquitin
          Botryllus schlosseri (Tunicata)
         Human Genetics
         Proteomics
         Molecular Medicine
      image:
      isPartOf:
         name:Cell and Tissue Research
         issn:
            1432-0878
            0302-766X
         volumeNumber:272
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Robert J. Lauzon
            affiliation:
                  name:Albany Medical College
                  address:
                     name:Department of Pediatrics, Albany Medical College, Albany, USA
                     type:PostalAddress
                  type:Organization
                  name:Albany Medical College
                  address:
                     name:Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, Albany, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chris W. Patton
            affiliation:
                  name:Hopkins Marine Station of Stanford University
                  address:
                     name:Hopkins Marine Station of Stanford University, Pacific Grove, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Irving L. Weissman
            affiliation:
                  name:Stanford University Medical Center
                  address:
                     name:Department of Pathology and Developmental Biology, Howard Hughes Medical Institute, Beckman Research Center, Stanford University Medical Center, Stanford, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cell and Tissue Research
      issn:
         1432-0878
         0302-766X
      volumeNumber:272
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Albany Medical College
      address:
         name:Department of Pediatrics, Albany Medical College, Albany, USA
         type:PostalAddress
      name:Albany Medical College
      address:
         name:Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, Albany, USA
         type:PostalAddress
      name:Hopkins Marine Station of Stanford University
      address:
         name:Hopkins Marine Station of Stanford University, Pacific Grove, USA
         type:PostalAddress
      name:Stanford University Medical Center
      address:
         name:Department of Pathology and Developmental Biology, Howard Hughes Medical Institute, Beckman Research Center, Stanford University Medical Center, Stanford, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Robert J. Lauzon
      affiliation:
            name:Albany Medical College
            address:
               name:Department of Pediatrics, Albany Medical College, Albany, USA
               type:PostalAddress
            type:Organization
            name:Albany Medical College
            address:
               name:Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, Albany, USA
               type:PostalAddress
            type:Organization
      name:Chris W. Patton
      affiliation:
            name:Hopkins Marine Station of Stanford University
            address:
               name:Hopkins Marine Station of Stanford University, Pacific Grove, USA
               type:PostalAddress
            type:Organization
      name:Irving L. Weissman
      affiliation:
            name:Stanford University Medical Center
            address:
               name:Department of Pathology and Developmental Biology, Howard Hughes Medical Institute, Beckman Research Center, Stanford University Medical Center, Stanford, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Pediatrics, Albany Medical College, Albany, USA
      name:Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, Albany, USA
      name:Hopkins Marine Station of Stanford University, Pacific Grove, USA
      name:Department of Pathology and Developmental Biology, Howard Hughes Medical Institute, Beckman Research Center, Stanford University Medical Center, Stanford, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(79)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.74s.

LINK . SPRINGER . COM {}