We are analyzing https://link.springer.com/article/10.1007/bf00294369.

Title:
Ferritin immunohistochemistry as a marker for microglia | Acta Neuropathologica
Description:
An immunohistochemical analysis of formalin-fixed, paraffin-embedded brain sections was performed with antisera against holoferritin and the light(L)-subunit of ferritin. Sections immunostained using anti-glial fibrillary acidic protein (GFAP), Ricinus communis agglutinin-1 (RCA-1) stain for microglia and iron stain (Berlin blue stain) were compared. The L-subunit of ferritin was purified from normal human spleen according to the modified scrapie-associated fibrils purification, and the antiserum was raised in a rabbit. Both ferritin antisera positively stained resting and, more markedly, reactive microglia, both of which were also stained with RCA-1 but not with GFAP. Ferritin-positive resting microglia were seen more abundantly in cerebral and cerebellar cortices than in white matter. The advantages of ferritin antisera over RCA-1 are as follows. (1) RCA-1 heavily stains blood vessels, while anti-ferritin does not, hence the microglial cells are more readily visualized with ferritin immunohistochemistry. (2) Reactive microglia and macrophages are more strongly stained with anti-ferritin. (3) The staining intensity of ferritin is independent of the length of tissue fixation in formalin. However, anti-ferritin is inferior to RCA-1 in staining resting microglia with a scanty cytoplasm, especially in the white matter, probably because the former recognizes cytoplasmic components, while the latter recognizes cell membrane. Iron stain only gave a reaction to microglial cells in brains with neurosyphilis and to hemosiderin-laden macrophages. Thus, in addition to RCA-1, ferritin antisera are useful as a microglia marker in formalin-fixed, paraffin-embedded sections.
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google, scholar, ferritin, microglia, human, cells, article, acta, brain, microglial, neuropathol, immunohistochemistry, rca, access, sci, usa, biochem, privacy, cookies, content, marker, kitamoto, tateishi, iron, resting, proc, natl, neurol, information, publish, research, search, kaneko, antisera, stain, normal, purification, matter, macrophages, drysdale, gene, acad, pathol, nature, berl, analysis, data, log, journal, sections,

Topics {✒️}

month download article/chapter free-radical-modified ferritin ferritin-positive resting microglia paraffin-embedded brain sections privacy choices/manage cookies full article pdf related subjects ricinus communis agglutinin-1 central nervous system recognizes cell membrane giant cell encephalitis endogenous glial cells identify microglial cells perivascular microglial cells amoeboid microglial cells microglial/astroglial cells counteract iron dyshomeostasis paraffin-embedded sections ferric oxyhydroxide core normal human spleen normal human microglia brain micro-vessels human ferritin gene european economic area scope submit manuscript recognizes cytoplasmic components hepcidin-ferroportin1 pathway astrocytic–neuronal crosstalk methanol-treated homogenate bone marrow-derived homozygous beta-thalassaemia creutzfeldt-jakob disease electron microscopic demonstration 15th annual meeting scrapie-infected mice amino acid sequence kyowa-hakko kogyo human spleen apoferritin conditions privacy policy human liver cells staining resting microglia aluminum-ferritin complex horse spleen ferritin human ferritin genes macrophage-specific antigen accepting optional cookies berlin blue stain human mononuclear phagocytes ferritin immunohistochemistry main content log

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         headline:Ferritin immunohistochemistry as a marker for microglia
         description:An immunohistochemical analysis of formalin-fixed, paraffin-embedded brain sections was performed with antisera against holoferritin and the light(L)-subunit of ferritin. Sections immunostained using anti-glial fibrillary acidic protein (GFAP), Ricinus communis agglutinin-1 (RCA-1) stain for microglia and iron stain (Berlin blue stain) were compared. The L-subunit of ferritin was purified from normal human spleen according to the modified scrapie-associated fibrils purification, and the antiserum was raised in a rabbit. Both ferritin antisera positively stained resting and, more markedly, reactive microglia, both of which were also stained with RCA-1 but not with GFAP. Ferritin-positive resting microglia were seen more abundantly in cerebral and cerebellar cortices than in white matter. The advantages of ferritin antisera over RCA-1 are as follows. (1) RCA-1 heavily stains blood vessels, while anti-ferritin does not, hence the microglial cells are more readily visualized with ferritin immunohistochemistry. (2) Reactive microglia and macrophages are more strongly stained with anti-ferritin. (3) The staining intensity of ferritin is independent of the length of tissue fixation in formalin. However, anti-ferritin is inferior to RCA-1 in staining resting microglia with a scanty cytoplasm, especially in the white matter, probably because the former recognizes cytoplasmic components, while the latter recognizes cell membrane. Iron stain only gave a reaction to microglial cells in brains with neurosyphilis and to hemosiderin-laden macrophages. Thus, in addition to RCA-1, ferritin antisera are useful as a microglia marker in formalin-fixed, paraffin-embedded sections.
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      headline:Ferritin immunohistochemistry as a marker for microglia
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