We are analyzing https://link.springer.com/article/10.1007/bf00285162.

Title:
Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum | Human Genetics
Description:
Interphase nuclei isolated from paraffin-embedded tissue of four normal brains were hybridized with biotinated repetitive DNA probes specific for the (peri) centromeric regions of chromosomes 1 and 7. Hybridization results were visualized with a peroxidase-DAB system after which the number of specific signals per nucleus was counted using bright field microscopy. Using the probe specific for chromosome 7 (p7t1), both the cerebral and the cerebellar samples showed 2 spots in 82% and 83%, respectively, of the nuclei. In situ hybridization with the chromosome 1 probe (pUC1. 77) showed two spots in 69% of the cerebral nuclei. In cerebellar samples, hybridization with pUC1.77 resulted in only one large spot per nucleus in 82% of the cells. The average spot size in nuclei with one signal was about 1.6 times as large as that in nuclei with two signals. These observations suggest that the single large spot in the cerebellar cells is not the result of monosomy of chromosome 1 but that it reflects somatic pairing of the two chromosome 1 centromeres. Based on the size and the fraction of nuclei with one large spot, the small granular neuron is the most likely candidate. The difference between cerebral and cerebellar samples indicates that this somatic pairing of chromosome 1 is a cell-type-dependent phenomenon.
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Keywords {🔍}

google, scholar, chromosome, human, interphase, article, nuclei, van, hybridization, genet, situ, dna, hum, content, probes, nucleus, cells, access, somatic, specific, cerebellar, nucleic, cremer, leiden, privacy, cookies, analysis, raap, ploeg, res, hopman, ahn, cell, cytometry, publish, search, pairing, centromeres, cerebellum, arnoldus, chromosomes, field, large, spot, open, manuelidis, satellite, acid, data, information,

Topics {✒️}

bright field microscopy month download article/chapter laser-uv-microbeam experiments laser-uv-microirradiation experiments cell-type-dependent phenomenon chromosome-specific dna probes paraffin-embedded pathological material alpha satellite dna reflects somatic pairing satellite dna sequences full article pdf privacy choices/manage cookies peroxidase-dab system related subjects distinct alphoid domains human cerebellum vertebrate somatic cells 2-acetylaminofluorene-modified probes human interphase nuclei centromeric regions paraffin-embedded tissue cellular dna content interphase cell nuclei check access instant access single-cell epigenomics cerebellar nuclei—core premature chromosome condensation individual chromosome domains european economic area scope submit manuscript small granular neuron somatic pairing sulfhydryl-hapten ligands solid bladder tumors biotin labeled polynucleotides sex chromosome positions chinese hamster cells neuroectodermal tumor cells conditions privacy policy accepting optional cookies human chromosome 7 indirect hybridocytochemical detection cancer genet cytogenet human tumors main content log chromosome 1 centromeres october 1989 volume 83 average spot size journal finder publish

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         headline:Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum
         description:Interphase nuclei isolated from paraffin-embedded tissue of four normal brains were hybridized with biotinated repetitive DNA probes specific for the (peri) centromeric regions of chromosomes 1 and 7. Hybridization results were visualized with a peroxidase-DAB system after which the number of specific signals per nucleus was counted using bright field microscopy. Using the probe specific for chromosome 7 (p7t1), both the cerebral and the cerebellar samples showed 2 spots in 82% and 83%, respectively, of the nuclei. In situ hybridization with the chromosome 1 probe (pUC1. 77) showed two spots in 69% of the cerebral nuclei. In cerebellar samples, hybridization with pUC1.77 resulted in only one large spot per nucleus in 82% of the cells. The average spot size in nuclei with one signal was about 1.6 times as large as that in nuclei with two signals. These observations suggest that the single large spot in the cerebellar cells is not the result of monosomy of chromosome 1 but that it reflects somatic pairing of the two chromosome 1 centromeres. Based on the size and the fraction of nuclei with one large spot, the small granular neuron is the most likely candidate. The difference between cerebral and cerebellar samples indicates that this somatic pairing of chromosome 1 is a cell-type-dependent phenomenon.
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            Field Microscopy
            Centromeric Region
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            Bright Field Microscopy
            Human Genetics
            Molecular Medicine
            Gene Function
            Metabolic Diseases
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      headline:Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum
      description:Interphase nuclei isolated from paraffin-embedded tissue of four normal brains were hybridized with biotinated repetitive DNA probes specific for the (peri) centromeric regions of chromosomes 1 and 7. Hybridization results were visualized with a peroxidase-DAB system after which the number of specific signals per nucleus was counted using bright field microscopy. Using the probe specific for chromosome 7 (p7t1), both the cerebral and the cerebellar samples showed 2 spots in 82% and 83%, respectively, of the nuclei. In situ hybridization with the chromosome 1 probe (pUC1. 77) showed two spots in 69% of the cerebral nuclei. In cerebellar samples, hybridization with pUC1.77 resulted in only one large spot per nucleus in 82% of the cells. The average spot size in nuclei with one signal was about 1.6 times as large as that in nuclei with two signals. These observations suggest that the single large spot in the cerebellar cells is not the result of monosomy of chromosome 1 but that it reflects somatic pairing of the two chromosome 1 centromeres. Based on the size and the fraction of nuclei with one large spot, the small granular neuron is the most likely candidate. The difference between cerebral and cerebellar samples indicates that this somatic pairing of chromosome 1 is a cell-type-dependent phenomenon.
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      dateModified:
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         Field Microscopy
         Centromeric Region
         Interphase Nucleus
         Bright Field Microscopy
         Human Genetics
         Molecular Medicine
         Gene Function
         Metabolic Diseases
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