We are analyzing https://link.springer.com/article/10.1007/bf00039030.

Title:
Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers | Plant Molecular Biology
Description:
Abscisic acid (ABA) inhibits the gibberellic acid induced synthesis of α-amylase in barley aleurone layers, yet ABA itself induces more than a dozen polypeptides (Lin & Ho, Plant Physiol 82: 289–297, 1986). As part of our effort to elucidate the molecular action of ABA in barley aleurone layers, we have isolated and characterized an ABA-induced cDNA clone, pHV A1. This cDNA clone hybridizes to an RNA species of approximately 1.1 kb from ABA-treated barley aleurone layers. The level of this mRNA is tripled within 40 minutes after ABA treatment, reaches a peak at 8–12 h, and is present up to 48 h. The induction of this mRNA responds to concentrations of ABA as low as 10-9 M, but higher ABA concentrations induce higher expression of this mRNA. The products of hybrid-select translation and in vitro transcription/translation with pHV A1 comigrate on SDS gel as a 27 kDa polypeptide. However, the sequence of pHV A1 indicates that it has an open reading frame encoding a 22 kDa protein. This size discrepancy is probably due to the high content of the basic amino acid, lysine. This notion has been confirmed by two-dimensional gel electrophoresis showing that this polypeptide is one of the most basic proteins in ABA-treated barley aleurone layers. The deduced amino acid sequence of pHV A1 contains nine imperfect repeats 11 amino acids long which share homology with cotton Lea 7 protein (Baker, Steele & Dure, Plant Mol Biol, in press). The identity and function of the encoded product of pHV A1 is under investigation.
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Keywords {🔍}

google, scholar, plant, barley, acid, aleurone, abscisic, layers, article, biol, molecular, aba, physiol, mrna, protein, biology, cloning, synthesis, mol, thd, content, cdna, expression, access, usa, dna, privacy, cookies, phv, sequence, press, gene, cells, publish, search, characterization, uknes, gibberellic, αamylase, action, open, high, amino, proteins, acids, embryos, proc, natl, acad, sci,

Topics {✒️}

month download article/chapter endogenous α-amylase inhibitor probable amylase/protease inhibitor barley amylase/subtilisin inhibitor aba-regulated gene expression aba-induced cdna clone cotton late embryogenesis-abundant barley aleurone layers rapid gene regulation cold spring harbor privacy choices/manage cookies cdna clone hybridizes full article pdf dna cloning techniques β-conglycinin expression barley aleurone cells double-stranded cdna plant mol biol storage-protein synthesis related subjects mature aleurone morphology basic amino acid recombinant dna molecules molecular biology heat-shock response molecular cloning dna fragments separated abscisic acid induction european economic area mrna rapidly-induced van vloten-doting zein cdnas obtained encephalomyocarditis virus-infected chain-terminating inhibitors developing wheat embryos conditions privacy policy july 1988 volume 11 hybrid-select translation messenger rna levels abscisic acid induces α-amylase wheat-germ agglutinin check access instant access accepting optional cookies article hong plant cells vitro transcription/translation nucleotide sequence analysis plant physiol 82

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