We are analyzing https://link.springer.com/article/10.1007/bf00021188.

Title:
Promoters from kin1 and cor6.6, two Arabidopsis thaliana low-temperature-and ABA-inducible genes, direct strong β-glucuronidase expression in guard cells, pollen and young developing seeds | Plant Molecular Biology
Description:
The ability of most higher plants to withstand freezing can be enhanced by cold acclimation, although the freezing tolerance of plant tissues is also affected by their developmental stage. In addition, low temperature has pleiotropic effects on many plant developmental processes such as vernalization. The interaction between plant development and low temperature implies that some genes are regulated by both environmental factors and developmental cues. Although a number of cold-inducible genes from plants have been identified, information concerning their regulation during plant development is limited. In order to understand their developmental regulation and obtain possible clues as to function, the promoters of kin1 and cor6.6, two cold- and abscisic acid (ABA)-regulated genes from Arabidopsis thaliana, were fused to the β-glucuronidase (GUS)-coding sequence and the resulting constructs were used to transform tobacco and A. thaliana. Transgenic plants with either the kin1 or cor6.6 promoter showed strong GUS expression in pollen, developing seeds, trichomes and, most interestingly, in guard cells. During pollen development, maximum GUS activity was found in mature pollen. In contrast, the maximum GUS activity during seed development was during early embryogenesis. These patterns of expression distinguish kin1 and cor6.6 from related lea genes which are strongly expressed during late embryogenesis. There was no major qualitative difference in patterns of GUS expression between kin1 and cor6.6 promoters and the results were similar for transgenic tobacco and Arabidopsis. Considering the results described, as well as those in an accompanying paper Wang et al., 1995, Plant Mol Biol 28: 605–617 (this issue), we suggest that osmotic potential might be a major factor in regulating the expression of kin1 and cor6.6 during several developmental processes. The implication of the results for possible function of the gene products is discussed.
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Keywords {🔍}

plant, google, scholar, arabidopsis, thaliana, mol, gene, genes, biol, expression, article, cold, development, kin, cor, analysis, plants, regulation, promoter, pollen, developmental, transgenic, physiol, molecular, wang, privacy, cookies, content, research, promoters, cutler, low, temperature, sequence, gus, access, stomatal, thomashow, information, publish, search, biology, guard, cells, seeds, acclimation, abscisic, acid, related, aba,

Topics {✒️}

month download article/chapter abscisic acid-responsive sequences desiccation-responsive rd29 gene aba-responsive promoter isolated arabidopsis thaliana low-temperature aba-inducible arabidopsis gene molecular genetics molecular biology aba-regulated gene expression national research council young developing seeds osmotin gene promoter full article pdf privacy choices/manage cookies rab-related gene low temperature implies aba-induced genes gene expression induced spinach gene responsive related subjects plant developmental processes related lea genes arabidopsis thaliana seeds aba-inducible genes brassica napus development male gametophyte development plant cold hardiness 193 bp promoter region stress-induced expression accompanying paper wang isolated epidermal strips arabidopsis thaliana cor15b transgenic tomato plants assaying chimeric genes european economic area maximum gus activity cis-elements responsible cis-acting elements datura stramonium embryos selected inorganic ions shared regulatory elements cold-inducible genes aba stress inducibility conditions privacy policy major qualitative difference ho t-hd arabidopsis thaliana cor15a article wang check access instant access

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      description:The ability of most higher plants to withstand freezing can be enhanced by cold acclimation, although the freezing tolerance of plant tissues is also affected by their developmental stage. In addition, low temperature has pleiotropic effects on many plant developmental processes such as vernalization. The interaction between plant development and low temperature implies that some genes are regulated by both environmental factors and developmental cues. Although a number of cold-inducible genes from plants have been identified, information concerning their regulation during plant development is limited. In order to understand their developmental regulation and obtain possible clues as to function, the promoters of kin1 and cor6.6, two cold- and abscisic acid (ABA)-regulated genes from Arabidopsis thaliana, were fused to the β-glucuronidase (GUS)-coding sequence and the resulting constructs were used to transform tobacco and A. thaliana. Transgenic plants with either the kin1 or cor6.6 promoter showed strong GUS expression in pollen, developing seeds, trichomes and, most interestingly, in guard cells. During pollen development, maximum GUS activity was found in mature pollen. In contrast, the maximum GUS activity during seed development was during early embryogenesis. These patterns of expression distinguish kin1 and cor6.6 from related lea genes which are strongly expressed during late embryogenesis. There was no major qualitative difference in patterns of GUS expression between kin1 and cor6.6 promoters and the results were similar for transgenic tobacco and Arabidopsis. Considering the results described, as well as those in an accompanying paper Wang et al., 1995, Plant Mol Biol 28: 605–617 (this issue), we suggest that osmotic potential might be a major factor in regulating the expression of kin1 and cor6.6 during several developmental processes. The implication of the results for possible function of the gene products is discussed.
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