We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-242-1_3.

Title:
In Vitro JAK Kinase Activity and Inhibition Assays | SpringerLink
Description:
The discovery that a range of myeloproliferative diseases and leukemias are associated with Janus Kinase (JAK) mutations has highlighted the importance of JAK/STAT signalling in disease and sparked a renewed interest in developing JAK inhibitors. In vitro kinase...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Telecommunications
Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

article, jak, pubmed, google, scholar, cas, kinase, tyrosine, protocol, scott, jakstat, signalling, babon, protein, privacy, cookies, content, information, publish, research, inhibition, assays, kinases, access, leukemia, cell, mutation, search, activity, jeffrey, murphy, mutations, inhibitors, wilks, harpur, shuai, darnell, nature, activation, constitutive, vera, gilliland, green, acute, lee, cancer, download, nhmrc, parkville, australia,

Topics {✒️}

jak/stat signaling pathway month download article/chapter sh2-phosphotyrosyl peptide interactions phosphotransferase-related catalytic domain haematology/structural biology divisions tel-jak2 fusion protein pcm1-jak2 fusion gene interferon-dependent tyrosine phosphorylation jak/stat signalling jak-stat signalling privacy choices/manage cookies constitutive kinase activity acute lymphoblastic leukaemias device instant download inhibition assays protocol rethinking jak2 inhibition vitro kinase assays tyrosine kinase jak2 protein tyrosine kinase wilks af pseudokinase domain target acute megakaryoblastic leukemia finally inhibition assays european economic area assess mutant forms altered enzymatic properties cytokine signal transduction levine rl campbell pj arc future fellowship author information authors eliza hall institute editor information editors royal parade 1g journal finder publish conditions privacy policy collins-underwood jr nhmrc program grant nhmrc project grant protein tyrosine kinases protein-tyrosine kinases constitutive signalling developing jak inhibitors janus kinase darnell je jr accepting optional cookies interferon activation le couedic jp polycythaemia vera based main content log

Schema {🗺️}

ScholarlyArticle:
      headline:In Vitro JAK Kinase Activity and Inhibition Assays
      pageEnd:55
      pageStart:39
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-242-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:JAK-STAT Signalling
         isbn:
            978-1-62703-242-1
            978-1-62703-241-4
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jeffrey J. Babon
            affiliation:
                  name:Walter and Eliza Hall Institute of Medical Research
                  address:
                     name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
                     type:PostalAddress
                  type:Organization
                  name:University of Melbourne
                  address:
                     name:University of Melbourne, Parkville, Australia
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:James M. Murphy
            affiliation:
                  name:Walter and Eliza Hall Institute of Medical Research
                  address:
                     name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:JAK, Kinase, Enzyme, Inhibition assay, Activation, Enzyme kinetics, JAK1, JAK2, JAK3, TYK2
      description:The discovery that a range of myeloproliferative diseases and leukemias are associated with Janus Kinase (JAK) mutations has highlighted the importance of JAK/STAT signalling in disease and sparked a renewed interest in developing JAK inhibitors. In vitro kinase assays are the most direct and quantitative method to assess mutant forms of JAK for altered enzymatic properties as well as verifying and quantifying the affinity and efficacy of potential inhibitors. Here, we describe protocols for heterologous expression and purification of JAK kinases from insect cells, assays to determine the activity of these purified kinases, and finally inhibition assays to determine the effectiveness of potential inhibitors.
      datePublished:2013
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:JAK-STAT Signalling
      isbn:
         978-1-62703-242-1
         978-1-62703-241-4
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Walter and Eliza Hall Institute of Medical Research
      address:
         name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
         type:PostalAddress
      name:University of Melbourne
      address:
         name:University of Melbourne, Parkville, Australia
         type:PostalAddress
      name:Walter and Eliza Hall Institute of Medical Research
      address:
         name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jeffrey J. Babon
      affiliation:
            name:Walter and Eliza Hall Institute of Medical Research
            address:
               name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
               type:PostalAddress
            type:Organization
            name:University of Melbourne
            address:
               name:University of Melbourne, Parkville, Australia
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:James M. Murphy
      affiliation:
            name:Walter and Eliza Hall Institute of Medical Research
            address:
               name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
      name:University of Melbourne, Parkville, Australia
      name:Cancer and Haematology/Structural Biology Divisions, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(94)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

5.11s.

LINK . SPRINGER . COM {}