We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-218-6_22.

Title:
Production of CD4+ and CD8+ T Cell Hybridomas | SpringerLink
Description:
T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Production of CD4+ and CD8+ T Cell Hybridomas
      pageEnd:307
      pageStart:297
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-218-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Antigen Processing
         isbn:
            978-1-62703-218-6
            978-1-62703-217-9
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:David H. Canaday
            affiliation:
                  name:Case Western Reserve University
                  address:
                     name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Antigen presentation, T cell hybridoma, Antigen processing, Macrophage, Dendritic cell
      description:T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma line. Unfused thymoma cells are eliminated by engineered metabolic selection. Antigen-specific hybridomas are identified and may be characterized in detail. Primary T cells are preferable for studies of the regulatory mechanisms intrinsic to T cells, but for study of antigen presentation T cell hybridomas have advantages over primary T cell clones, including their relative uniformity, stability over time, and ready availability in large numbers for extensive antigen presentation experiments.
      datePublished:2013
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Antigen Processing
      isbn:
         978-1-62703-218-6
         978-1-62703-217-9
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Case Western Reserve University
      address:
         name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:David H. Canaday
      affiliation:
            name:Case Western Reserve University
            address:
               name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
WebPageElement:
      isAccessibleForFree:
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